Abstract
Mouse hybridoma cells producing monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the non-woven polyester fabric (NWPF) discs in stationary and stirred conditions. Firstly, a series of batch experiments were performed in Petri dishes with and without NWPF disc, containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. The results showed that in the presence of the NWPF discs cell viability, cell yield and total monoclonal antibody production was higher than that of the disc-free culture. In the second part, reactor cultures were realized in a 1L spinner basket containing NWPF discs. During the culture the cell growth and MAb production were examined. The highest MAb concentration were obtained in spinner basket. All results indicated that NWPF-containing spinner basket can be used for the large-scale production of MAb.
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© 2003 Springer Science+Business Media Dordrecht
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Aslankaraoğlu, E., Gümüşderelioğlu, M., Yilmaz, Ş., Bingöl, N. (2003). Proliferation and Monoclonal Antibody Production of Hybridoma Cells Immobilized on Non-Woven Polyester Fabric Discs. In: Yagasaki, K., Miura, Y., Hatori, M., Nomura, Y. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0726-8_34
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DOI: https://doi.org/10.1007/978-94-017-0726-8_34
Publisher Name: Springer, Dordrecht
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