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Laboratory scale bioreactors for study of fungal physiology and metabolism in solid state fermentation system

Bioreacteurs de laboratoire pour étudier la physiologie et le métabolisme de champignons cultivés en milieu solide

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Advances in Solid State Fermentation

Summary

Data on physiology, effect of various parameters on the growth as well as on the metabolism of the culture, monitoring and regulation of parameters are of critical importance in the design of a commercial process. The studies on these aspects at laboratory scale thus dictate the use of efficient laboratory scale bioreactors. A column bioreactor was developed at ORSTOM and the manufactured commercial units are, herewith, described along with various strategies for monitoring and control of different parameters. The design of the modified kneading machine is also reported for upgradation of wastes at a level of 120 L capacity. To overcome the lack of a system for inoculum development, a disk fermentor was developed, which was found to be highly efficient. A static fermenter of the capacity of 50 Kg substrate, named as Zymotis was developed for efficient control of the fermentation temperature and achievement of high productivities. All these bioreactors form one of the most important contribution of ORSTOM to solid state fermentations, based on the use of solid substrates/supports.

Resume

Les données de la physiologie, l’effet de différents paramètres sur la croissance aussi bien que sur le métabolisme de la culture, la supervision et la régulation des paramètres sont d’une importance critique dans la conception de procédés commerciaux. Les études concernant ces aspects à l’échelle du laboratoire impliquent donc l’utilisation des bioréacteurs de laboratoire efficaces. Le bioréacteur en colonne développé à l’ORSTOM et l’unité commerciale construite sont ici décrits, de même que les stratégies de contrôle et supervision de différents paramètres. La conception d’un pétrin de boulangerie modifié est également signalée pour la valorisation de déchets à une capacité de 120 litres. Afin de combler la manque de systèmes de production contrôlée d’inoculum, un fermenteur à disques fut développé, et se révéla d’une grande efficacité. Un fermenteur statique d’une capacité de 50 kg de substrat, nommé Zymotis, fut développé dans le but d’obtenir un contrôle efficace de la température de fermentation et d’atteindre des productivités élevées. Tous ces bioréacteurs forment l’une des plus importantes contributions de l’ORSTOM aux fermentations en milieu solide, basées sur l’utilisation de substrats/supports solides.

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De Araujo, A.A., Lepilleur, C., Delcourt, S., Colavitti, P., Roussos, S. (1997). Laboratory scale bioreactors for study of fungal physiology and metabolism in solid state fermentation system. In: Roussos, S., Lonsane, B.K., Raimbault, M., Viniegra-Gonzalez, G. (eds) Advances in Solid State Fermentation. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0661-2_8

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  • DOI: https://doi.org/10.1007/978-94-017-0661-2_8

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4904-9

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