Summary
A new culture technique was developed for studying the physiology of Pleurotus mycelium in solid state fermentation (SSF) system. This method consists of utilization sugar cane bagasse, a by-product of sugar industry, as solid support for absorbing a liquid culture medium. Compared with classical techniques, it offers the advantage of better control of liquid medium composition as well as culture conditions. Fermenters were coupled with on-line gas analysis by using a gas chromatograph equipped with thermal conductivity detector. Solid state culture were aerated at a very low flow air rate (0.04 ml/g moist solids). Concentrations of CO2 and O2 in the exhaust air from fermenters were continuously analysed. By means of this technique, culture conditions were optimized respect to the ratio urea/ammonium sulphate, the particle size of bagasse and inoculation rate. Under best conditions, nearly 90% of substrate was consumed in 6 days. This culture technique offers, excellent practicability for the development of mycelium inocula for commercial exploitation of Pleurotus spp.
Resume
Une nouvelle technique a été mise au point pour étudier la physiologie de croissance mycélienne de Pleurotus opuntiae cultivé en milieu solide. Cette méthode consiste à utiliser la bagasse de canne à sucre comme support solide pour y absorber une solution nutritive. Comparée à des techniques classiques, cette méthode offre des avantages pour un meilleur contrôle de la composition des solutions nutritives tout en respectant les conditions définies de fermentation en milieu solide. Les cultures en milieu solide ont été aérées avec un très faible débit d’air (0.04 mi/g de milieu humidifié). Les concentrations en CO2 et en O2 ont été analysées à partir des prélèments automatiques des effluents gazeux à la sortie des fermenteurs en utilisant un chromatographe de gaz équipé d’un détecteur à conductivité thermique. Par cette technique, les conditions de culture ont été optimisées en particulier le rapport urée/sulfate d’ammonium, la taille des particules du support et de l’inoculum. Sous ces conditions, environ 90% du substrat carboné a été consommé en 6 jours. Cette technique de culture offre des perspectives interessantes pour la production de mycélium de Pleurotes et peut être utilisée pour une exploitation commerciale.
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References
Aslam Azizi, K., Shamala, T.R. and Sreekantiah, KR. 1990. Cultivation of Pleurotus sajor-caju on certain agro-industrial wastes and utilization of the residues for cellulase and D-xylanase production. Mush. J. Tropics, 10: 21 – 26.
Bisaria, R., Madan, M. and Bisaria, V.S. 1987. Mineral content of the mushroom Pleurotus sajor-caju cultivated on different agro-residues. Mush. J. Tropics, 7: 53 – 60.
Chang S.T. and Miles 1991. World production of edible mushrooms. Muschroo J. 504: 15 – 15.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chem. 28: 350 – 356.
Fritsche, G. 1981. Some remarks on the breeding, maintenance of strains and spawn of Agaricus bisporus and A. bitorquis. Mushroom Science XI. Proceedings of the Eleventh International Scientific Congress on the cultivation of Edible Fungi, Australia, pp. 367 – 385.
Guillen, F., Martinez, A.T. and Martinez, M.J. 1990. Production of hydrogen peroxide by aryl-alcohol oxidase from the ligninolytic fungus Pleurotus eryngii. Appl. Microbiol. Biotechnol., 32: 465 – 469.
Goltapeh, E.M. and Kapoor, J.N. 1981. New substrates for spawn production of button mushroom Agaricus bisporus (Lange) Singer. Mushroom Science XI. Proceedings of the Eleventh International Scientific Congress on the cultivation of Edible Fungi, Australia, pp. 281 – 285.
Gupta, V.K. and Langar, P.N. 1988. Pleurotus florida for upgrading the nutritive value of wheat straw. Biological Wastes, 23: 57 – 64.
Guzman, G. and Martinez-Carrera, D. 1986. Pleurotus growing on coffee-pulp in a semi-industrial plant. Mush. J. Tropics, 3: 7 – 10.
Kannan, K. and Oblisami, G. 1990. Enzymology of ligno-cellulose degradation by Pleurotus sajor-caju during growth on paper-mill sludge. Biological Wastes, 33: 1 – 8.
Khanna, P. and Garcha, H.S. 1985. Physiological studies on Pleurotus spp. I. Nitrogen utilization. Mush. Newsletter Tropics, 5: 16 – 21.
Martinez, A.T., González, A.E., Martinez, M.J., Guillen, F. and Barrasa, J.M. 1992. Biotecnologfa en la industria papelera: utilización de los hongos y sus enzimas en la fabricación de papel y en el tratamiento de efluentes. Rev. Iberoam. Micol. 9: 76 – 80.
Martinez-Carrera, D., Guzman, G. and Soto, C. 1985. The effect of fermentation of coffee pulp in the cultivation of Pleurotus ostreatus in México. Mush. J. Tropics, 1: 21 – 28.
Martinez-Carrera, D., Morales, P., Soto, C., Murrieta, E. and Guzman, G. 1986. Cultivo de Pleurotus ostreatus sobre hojas usadas en la extracción de aceites esenciales. Rev. Mex. de Micologia, 2: 119 – 124.
Martinez-Carrera, D., Morales, P. and Sobal, M. 1989. Produccion de hongos comestibles sobre pulpa de café a nivel comercial. In Roussos, S., Licona, F.R. and Gutierrez, R.M. (Eds.), I Seminario Internacional de Biotecnologia en la Industria Cafetalera. Memorias, Xalapa, Ver. Mexico, p. 177 – 184.
Martinez-Ortiz, P. and Roussos, S. 1991. Production de blanc de Pleurotus cornucopiae par fermentation en milieu solide sur rafles de mais au Mexique: Mise au point de la méthode de culture en FMS. Projet MRT/ORSTOM N°90.L.0724. Rapport N°2, ORSTOM, Montpellier.
Olivier, J.M., Laborde, J., Guinbertau, J. and Poitou, N. 1991. La culture dr champignons, Armand Colin, Paris.
Oriol, E., Schettino, B., Viniegra-González, G. and Raimbault, M. 1988. Solid state culture of Aspergillus niger on support. J. Ferment. Technol. 66: 57 – 62.
Raimbault, M. and Alazard, D. 1980. Culture method to study fungal growth in solid fermentation. European J. Appl. Microbiol., 9: 199 – 209.
Roussos, S., Martinez, P. and Olivier, J-M 1990. Production de blanc de Pleurotus cornucopiae par fermentation en milieu solide sur rafles de maïs au Mexique. Projet MRT/ORSTOM N° 90.L.0724. Rapport N°I, ORSTOM, Montpellier.
Roussos, S., Raimbaul,t M., Saucedo-Castañeda, G., Viniegra-González, G. and Lonsane, B.K. 1991. Kinetics and ratios of carboxy-methyl cellulase and filter paper activities of the cellulolytic enzymes produced by Trichoderma harzianum on different substrates in solid state fermentation. Micol. Neotrop. Apl. 4: 19 – 40.
Sannia, G., Limongi, P., Cocca, E., Buonocore, F., Nitti, G. and Giardina, P. 1991. Purification and characterization of a veratryl alcohol oxidase enzyme from the lignin degrading basidiomycete Pleurotus ostreatus. Biochim. et Biophys. Acta, 1073: 114 – 119.
Saucedo-Castañeda, G., Lonsane, B.K., Navarro, J.M., Roussos, S. and Raimbault, M. 1992. Potential of using a simple fermenter for biomass built up, starch hydrolysis and ethanol production: Solid state fermentation system involving Schwanniomyces castellii, Appl. Biochem. Biotechnol. 36: 47 – 61.
Saucedo-Castañeda, G., Trejo-Hernandez, M.R., Lonsane, B.K., Navarro, J.M., Roussos, S., Dufour, D. and Raimbault, M. 1993. On-line monitoring and control system for CO, and 02 concentrations in aerobic and anaerobic solid state fermentations. Process Biochem. 29: 13 – 24.
Soccol, C., Marin, B., Raimbault, M. and Lebeault, J.M. 1994. Breeding and growth of Rhizopus in raw cassava by solid state fermentation. Appl. Microbiol. Biotechnol. 41: 330 – 336.
Trejo-Hernandez, M.R., Lonsane, B.K., Raimbault, M. and Roussos, S. 1993. Spectra of ergot alkaloids produced by Claviceps purpurea 1029c in solid state fermentation system: Influence of the composition of liquid medium used for impregnating sugar cane pith bagasse. Process Biochem. 28: 23 – 27.
Valsameda, M., Martinez, M.J. and Martinez, A.T. 1991. Kinetics of wheat straw solid-state fermentation with Trametes versicolor and Pleurotus ostreatus - lignin and polysaccharide alteration and production of related enzymatic activities. Appl. Microbiol. Biotechnol., 35: 817 – 823.
Yang, Q.Y. and Jong, S.C. 1987. A quick and efficient method of making mushroom spawn. Proceedings of the Twelfth International Congress on the Science and Cultivation of Edible Fungi. Braunschweig, Germany, pp. 317 – 324.
Zadrazil, F. 1975. Influence of CO, concentration on the mycelium growth of three Pleurotus species. Europ. J. Appl. Microbiol. 1: 327 – 335.
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Roussos, S., Bresson, E., Saucedo-Castañeda, G., Martinez, P., Guinberteau, J., Olivier, J.M. (1997). Production of mycelial cell inoculum of Pleurotus opuntiae on natural support in solid state fermentation. In: Roussos, S., Lonsane, B.K., Raimbault, M., Viniegra-Gonzalez, G. (eds) Advances in Solid State Fermentation. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0661-2_40
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DOI: https://doi.org/10.1007/978-94-017-0661-2_40
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