Summary
Lipase production by Rhizopus delemar has been studied by growing on a polymeric resin (Amberlite IRA 900), after absorbing a medium previously optimized in solid state fermentation (SSF) and also in submerged fermentation (SmF). The activity of R. delemar lipase, produced in liquid culture (LC), could be adsorbed to an extent of 24% on 1 g Amberlite. Desorption, carried out using 5 g NaCl / g Amberlite at pH 5, allowed to recover 35% of the adsorbed lipase. Data indicated that the resin displayed a thermoprotective effect, since only little loss in activity was observed, when the adsorbed enzyme was heated at 80°C for 24 hours. In solid state fermentation (SSF), the fungus produced high amounts of enzyme (93 U/g dry Amberlite at 24 hours, against 14.1 U/ml at 48 hours in submerged culture), when dextrin was used as a carbon source. Significant activity was also detected with maltose and surprisingly with glucose (68 and 57 U/g dry Amberlite, respectively). The strong inhibitory effect of glucose, observed in liquid culture, was reduced in SSF.
Resume
La production de lipases, par Rhizopus delemar cultivé sur un support synthétique (Amberlite IRA 900) imprégné d’une solution nutritive optimisée, a été étudiée d’abord en fermentation en milieu solide (FMS) et ensuite en fermentation submergée (SmF). L’activité lipase de R.delemar, produite en SmF, a pu être absorbée à 24% pour 1 g d’Amberlite. La desorption, réalisée en utilisant 5 g de NaCl/ g d’Amberlite à un pH 5, a permis de récupérer 35% de la lipase absorbée. Les résultats indiquent que la résine montre un effet thermo-protecteur vis-à-vis des lipases, étant donné qu’une faible perte d’activité enzymatique a été observée lorque l’enzyme a été absorbée et portée à 80°C pendant 24 h. En milieu solide (FMS) en présence de la dextrine comme source de carbone, le champignon filamenteux produit des quantités élevées d’enzymes (93 U/g poids sec d’Amberlite à 24 h, au lieu de 14,1 U/ml à 48h en fermentation submergée (SmF). Des activités importantes ont été détectées avec du maltose ou avec du glucose (68 et 57 U/g poids sec d’Amberlite, respectivement). L’hinibition forte provoquée par le glucose a été uniquement observée pour les cultures liquides alors que cette inhibition été nettement réduite en FMS.
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Christen, P., Angeles, N., Farres, A., Revah, S. (1997). Lipase production by Rhizopus delemar grown on a synthetic support in solid state fermentation. In: Roussos, S., Lonsane, B.K., Raimbault, M., Viniegra-Gonzalez, G. (eds) Advances in Solid State Fermentation. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0661-2_26
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DOI: https://doi.org/10.1007/978-94-017-0661-2_26
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