Summary
Optimization of the culture conditions of Aspergillus carbonarius (Asca), Aspergillus sp (C11B52) and Penicillium sp (V26A25), cultured in coprah cake solid state fermentation (SSF), was undertaken, using experimental matrices. The effects of seven culture parameters (humidity = F1, initial (NH4)2SO4, CaCl2 and MgSO4.7H20 concentrations = F2, F6, and F7, respectively, initial pH = F3, inoculum size = F4 and temperature = F5) on spore germination time and respiratory activities of the strains were studied. In general, the shortest lag phase was observed under the lowest values of the F1, F2, F3 and F7 parameters. Under these conditions Aspergillus carbonarius (Asca) and Aspergillus sp (C11B52) showed the highest respiratory activities (6.3% and 10.2%, respectively), while the highest respiratory activity (≈ 5%) of Penicillium sp was observed at the lower values of F2, F3, F4 and F5 parameters.
Resume
L’optimisation des conditions de culture d’Aspergillus carbonarius (Asca), Aspergillus sp (C11B52) et Penicillium sp (V26A25), cultivés sur tourteau de coprah en milieu solide, a été réalisée en utilisant des plans d’expérience. Les effets de sept paramètres de culture (humidité = F1, concentrations initiales de (NH4)2SO4, CaCl2 et de MgSO4.7H2O = F2, F6, et F7 respectivement, pH initial = F3, taille d’inoculum = F4 et température = F5) sur le temps de germination de spores et les activités respiratoires des souches ont été étudiés. Les temps les plus courts pour la germination de spores ont été obtenus pour les valeurs les plus faibles des facteurs Fl, F2, F3 et F7. Sous ces conditions expérimentales Aspergillus carbonarius et Aspergillus sp. ont montré les plus grandes activités respiratoires (6,3% et 10,2% respectivement) alors que l’activité respiratoire la plus élevée (≈ 5%) observée pour Penicillium sp. a été obtenue avec les plus faibles valeurs des paramètres F2, F3, F4 et F5.
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© 1997 Springer Science+Business Media Dordrecht
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Marakis, S.G., Lambraki, M., Perraud-Gaime, I., Hannibal, L., Roussos, S. (1997). Effects of several factors on fungal spore germination in solid state fermentation of coprah cake. In: Roussos, S., Lonsane, B.K., Raimbault, M., Viniegra-Gonzalez, G. (eds) Advances in Solid State Fermentation. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0661-2_15
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DOI: https://doi.org/10.1007/978-94-017-0661-2_15
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