Skip to main content

Pressure Drop as a Method to Evaluate Mold Growth in Solid State Fermentors

  • Chapter

Abstract

The measurement of pressure drop (DP) across an aerated fermentation bed was used to follow Aspergillus niger growth on Amberlite IRA-900, a synthetic resin, imbibed with a solution containing high concentrations of sucrose (Si=100, 200, 300 and 400 g l−1). The DP allowed monitoring the germination, vegetative growth, limitation and sporulation for the four concentrations studied A relation between the biomass and the relative intrinsic permeability established that the continuity of the gas phase was not broken and a regular mesh-like growth occurred Relative intrinsic permeability as low as 0.0125 occurred at biomass concentrations of 103 mg dry biomass (g dry support)−1 obtained with Si=400 g l−1. Under these conditions the mold occupies 34% of the free space. The DP measurement was used to follow growth on cane bagasse and wheat bran, in both cases, the four growth phases (germination, vegetative, substrate limitation, and sporulation) were monitored.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Laukevics J.J., Apsite A., Viesturs, U. and Tengerdy R. (1984) Solid Substrate Fermentation of Wheat Straw to Fungal Protein.Biotechnol. Bioeng. 26; 1465–1474

    Article  PubMed  CAS  Google Scholar 

  2. Lonsane B.K., Saucedo-Castaneda G., Raimbault M., Roussos S., Viniegra-Gonzalez G., Ghildyal N.P., Ramakrishna M., Krishnaiah M.M. (1992) Scale-up strategies for solid state fermentation systems. Process Biochemistry. 27: 259–273.

    Article  CAS  Google Scholar 

  3. Durand A., De la Broise D., Blachère H. 1988. Laboratory scale bioreactor for solid state processes.J Biotech. 8: 59–66.

    Article  CAS  Google Scholar 

  4. Sargantanis J., Karim M., Murphy V., Ryoo D., Tengerdy (1993) Effect of Operating Conditions on Solid Substrate Fermentation. Biotechnol. Bioeng. 42; 149–158.

    Article  PubMed  CAS  Google Scholar 

  5. Narahara, H., Koyama, Y., Yoshida,T. Pichangkura, S., Ueda, R., Taguchi, H. (1982) Growth and enzyme production in solid-state culture of Aspergillus oryzae. J. Ferment. Technol. 60: 311–319.

    CAS  Google Scholar 

  6. Barstow, L.M., Dale, B.E., Tengerdy, R.P. (1988) Evaporative temperature and moisture control in solid substrate fermentation. Biotechnol. Tech. 2: 237–242.

    Article  Google Scholar 

  7. Durand, A, Chereau, D. (1988) A new pilot reactor for solid state fermentation: Application to the protein enrichment of sugar beet pulp. Biotechnol. Bioeng. 31: 476–486.

    Article  PubMed  CAS  Google Scholar 

  8. Oriol, E., Schettino, B., ViniegraGonzales, G., Raimbault, M. (1988) Solid-state culture of Aspergillus niger on support. J. Ferment. Technol. 66: 57–62.

    Article  CAS  Google Scholar 

  9. Gervais, P. (1989) New sensor allowing continuous water activity measurement of submerged or solid-substrate fermentations. Biotechnol. Bioeng. 33, 266–271.

    Article  PubMed  CAS  Google Scholar 

  10. Sato K., Nagatani, M., Sato, S. (1982) A method of supplying moisture to the medium in a Solid-state culture with forced aeration. J. Ferment. Technol. 60: 607–610.

    CAS  Google Scholar 

  11. Desgranges, C., Georges, M., Vergoignan, C., Durand, A. (1991) Biomass estimation in solid state fermentation. Appl. Microbiol. Biotechnol. 35: 206–209.

    CAS  Google Scholar 

  12. Sato, K., Nagatani, M., Nakamura, K.I., Sato S. (1983) Growth estimation of Candida lipolytica from oxygen uptake in a solid state culture with forced aeration, J. Ferment. Technol. 61: 623–629.

    CAS  Google Scholar 

  13. Weng, Y., Hotchkiss, J.H. (1991) Headspace gas composition and chitin content as measures of Rhizopus stolonifer growth. J. Food Sci. 56: 274–285.

    Article  CAS  Google Scholar 

  14. Villegas E., Aubague S., Alcantara L. Auria R. y Revah S. (1993) Solid State Fermentation: Acid Protease Production in Controlled CO2 and O2 Environments. Biotech. Adv., 11, 387–397.

    Article  CAS  Google Scholar 

  15. Raimbault, M. (1980) Fermentation en milieu solide: Croissance de champignons filamenteux sur substrat amylacé, Thèse de Doctorat, U.P.S. Toulouse, France.

    Google Scholar 

  16. Davey, C.L., Penaloza, W., Kell, D.B., Hedger, J.N. (1991) Real-time monitoring of the accretion of Rhizopus oligosporus biomass during the solid-subtrate tempeh fermentation. World Journal of Microbiology. and Biotechnology. 7: 248–259.

    Article  Google Scholar 

  17. Laukevics, J., Apsite, A.F., Viesturs, U.S., Tengerdy, R.P. (1985) Steric hindrance of growth of filamentous fungi in solid substrate fermentation of wheat straw. Biotechnol. Bioeng. 27: 1687–1691.

    Article  PubMed  CAS  Google Scholar 

  18. Auria, R., Hernandez, S., Raimbault, M., Revah, S. (1990) Ion exchange resin: A model support for solid state growth fermentation of Aspergillus niger. Biotechnol. Techn. 4: 391–396.

    Article  CAS  Google Scholar 

  19. Auria R., Morales M., Villegas E. and Revah S. (1993) Influence of Mold Growth on the Pressure Drop in Aerated Solid State Fermentors. Biotechnol. Bioeng. 41, 1007–1013

    Article  PubMed  CAS  Google Scholar 

  20. Gumbira Sa’id E., Greenfield P. F., Mitchell D. A and Doelle H. W. (1993) Operational parameters for packed beds in solid-state cultivation. Biotech. Adv. 11, 599–610

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Auria, R., Revah, S. (1994). Pressure Drop as a Method to Evaluate Mold Growth in Solid State Fermentors. In: Galindo, E., Ramírez, O.T. (eds) Advances in Bioprocess Engineering. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0641-4_40

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-0641-4_40

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4459-4

  • Online ISBN: 978-94-017-0641-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics