Epr Analysis of Photosystem I Degradation During Photoinhibition of Isolated Chloroplast Membranes

  • C. P. Santos
  • R. Cammack
  • D. O. Hall

Abstract

As we have shown [1] continuous illumination of freshly isolated chloroplast membranes led to the decay of PSII activity with time, while PSI activity seemed relatively stable over the first 4–6 hours. However using previously frozen chloroplast membranes (-10° C) caused a decrease in the PSI stability and led to a decay of PSI activity during continuous illumination. This PSI decay of frozen and thawed membranes during photo-inhibition was correlated with the degradation of CPI (a chlorophyll-protein band in SDS gel electrophoresis. This suggests that PSI decay was due to the destruction of the photosystem I reaction centre.

Keywords

Electron Spin Resonance Methyl Viologen Continuous Illumination Signal Size Chloroplast Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Santos, C.P. and Hall, D.O. (1982) Plant Physiol. 70, 795–802.CrossRefGoogle Scholar
  2. 2.
    Evans, M.C.W. (1982) in Iron-sulphur proteins (Spiro, T.G. ed.), Vol.4, pp.249–284, John Wiley & Sons, New York.Google Scholar
  3. 3.
    Chamorovsky, S.K. and Cammack, R. (1982) Biochim. Biophys. Acta 678, 146–155.Google Scholar
  4. 4.
    Malkin, R. and Bearden, A.J. (1971) Proc. Natl. Acad. Sci. USA, 68, 16–19.CrossRefGoogle Scholar
  5. 5.
    Evans, M.C.W., Reeves, S.G. and Cammack R. (1974) FEBS Lett. 49, 111–114.CrossRefGoogle Scholar
  6. 6.
    Evans, M.C.W., Sihra, C.K., Bolton, J.R. and Cammack, R. (1975) Nature 256, 668–670.CrossRefGoogle Scholar
  7. 7.
    Cammack, R., Ryan, M.D. and Stewart, A.C. (1979) FEBS Lett., 107, 422–426.CrossRefGoogle Scholar
  8. 8.
    Mathis, P. and Sauer K. (1978) FEBS Lett., 88, 275–278.CrossRefGoogle Scholar
  9. 9.
    Golbeck, J.H. and Warden, J. (1982) Biochim. Biophys. Acta 681, 77–84.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1987

Authors and Affiliations

  • C. P. Santos
    • 1
    • 2
  • R. Cammack
    • 1
    • 2
  • D. O. Hall
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of Rio de JaneiroRio de JanerioBrazil
  2. 2.Department of BiologyKing’s CollegeLondonUK

Personalised recommendations