Epr Analysis of Photosystem I Degradation During Photoinhibition of Isolated Chloroplast Membranes

  • C. P. Santos
  • R. Cammack
  • D. O. Hall


As we have shown [1] continuous illumination of freshly isolated chloroplast membranes led to the decay of PSII activity with time, while PSI activity seemed relatively stable over the first 4–6 hours. However using previously frozen chloroplast membranes (-10° C) caused a decrease in the PSI stability and led to a decay of PSI activity during continuous illumination. This PSI decay of frozen and thawed membranes during photo-inhibition was correlated with the degradation of CPI (a chlorophyll-protein band in SDS gel electrophoresis. This suggests that PSI decay was due to the destruction of the photosystem I reaction centre.


Electron Spin Resonance Methyl Viologen Continuous Illumination Signal Size Chloroplast Membrane 
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Copyright information

© Springer Science+Business Media Dordrecht 1987

Authors and Affiliations

  • C. P. Santos
    • 1
    • 2
  • R. Cammack
    • 1
    • 2
  • D. O. Hall
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of Rio de JaneiroRio de JanerioBrazil
  2. 2.Department of BiologyKing’s CollegeLondonUK

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