Abstract
We investigated proton flow through the integral chloroplast ATP synthase, CF0-CF1, and also through its channel portion, CFO, alone. Proton pumps of thylakoids were pulse stimulated by single-turnover flashes of light and the backflow of protons was completely tracked by measurements in three subcompartments, in the lumen (pH-transients via neutral red), in the membrane (electric transients via electrochromism) and in the suspending medium (pH-transients via hydrophilic indicator dyes). It was noteworthy that these three ways to follow proton flow yielded consistent results, in other words, a proton which had left the lumen was detectable as a charge crossing the thylakoid membrane and as a proton appearing in the medium.
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Junge, W., Schoenknecht, G., Lill, H. (1987). Complete Tracking of Proton Flow Mediated by CF0-CF1 and by CF0. In: Biggins, J. (eds) Progress in Photosynthesis Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0516-5_29
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DOI: https://doi.org/10.1007/978-94-017-0516-5_29
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