Abstract
A significant portion of the catalytic site of CF0-CF1 is located on the β subunit (for a review, see (1)). This enzyme contains three copies of the β subunit which suggests that there are three active sites. Experiments that involve isotope exchange (2), substrate trapping (3) and binding of Mn (4) suggest that at least two of the active sites are coupled. Soluble CF1 is a latent ATPase that can be activated by a variety of procedures (5), some of which are believed to require the exchange of disulfide bonds of the γ subunit (6).
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References
Amzel, S. and Pedersen, P. (1985) Ann.Rev.Biochem. 52, 801–824
Kohlbrenner, W. and Boyer, P. (1983) J.Biol.Chem. 258, 10881–10886
Grubmeyer, C. and Penefsky, H. (1981) J.Biol.Chem. 256, 3728–3734
Hiller, R. and Carmeli, C. (1985) J.Biol.Chem. 260, 1614–1617
Nelson, N. (1976) Biochem.Biophys.Acta 45, 314–338
Nalin, C. and McCarty, R. (1984) J.Biol.Chem. 259, 7275–7280
Futai, M. (1977) Biochem.Biophys.Res.Comm. 79, 1231–1237
Philosoph, S., Binder, A. and Gromet-Elhanan, Z. (1977) J.Biol.Chem. 252, 8747–8752
Khananshvilli, D. and Gromet-Elhanan, Z. (1982) J.Biol.Chem. 257, 11377–11383
Yoshida, M., Sone, N., Hirata, H. and Kagawa, Y. (1977) J.Biol.Chem. 252, 3480–3485.
Khananshvilli, D. and Gromet-Elhanan, Z. (1984) FEBS Lett. 178, 10–14
Gromet-Elhanan, Z. and Khananshvilli, D. (1984) Biochemistry 23, 1022–1028
Harris, D., Boork, J. and Baltscheffsky, M. (1985) Biochemistry 24, 3876–3883
Pecoraro, V., Hermes, J. and Cleland, W. (1984) Biochemistry 23, 5262–5271
Frasch, W. and Selman, B. (1982) Biochemistry 21, 3636–3642
Cleland, W. (1967) Advances Enzymol. 29, 1–36
Chua, N-H. (1980) Methods in Enzymol. 69, 434–446
Sammons, W., Adams, L. and Nishizawa, E. (1981) Electrophoresis 2, 135–141
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© 1987 Springer Science+Business Media Dordrecht
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Apel, I.J., Mejia, A., Frasch, W.D. (1987). Purification of the β Subunit of the Chloroplast H+-Translocating ATPase of Spinach Thylakoids. In: Biggins, J. (eds) Progress in Photosynthesis Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0516-5_17
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DOI: https://doi.org/10.1007/978-94-017-0516-5_17
Publisher Name: Springer, Dordrecht
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