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DNA amplification in doubled haploids of Nicotiana

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In Vitro Haploid Production in Higher Plants

Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 24))

Abstract

Tobacco (Nicotiana tabacum L.) was the first crop species in which anther culture was successfully utilized for haploid production. Numerous research efforts aimed at identifying anther culture conditions necessary for maximizing yields of haploids have been carried out in this species. As a result of this work, the methodology required for generating large numbers of haploids of most tobacco genotypes has been available for over 20 years. In addition, both colchicine (Burk et al., 1972) and callus culture (Kasperbauer and Collins, 1972) techniques are available for chromosome doubling.

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References

  • Arcia, M.A., E.A. Wernsman and L.G. Burk, 1978. Performance of anther-derived dihaploids and their conventionally inbred parents as lines, in F1 hybrids, and in F2 generations. Crop Sci. 18: 413–418.

    Google Scholar 

  • Brown, J.S. and E.A. Wernsman, 1982. Nature of reduced productivity of anther-derived dihaploid lines of flue-cured tobacco. Crop Sci. 22: 1–4.

    Article  Google Scholar 

  • Brown, J.S., E.A. Wernsman and R.J. Schnell II, 1983. Effect of a second cycle of anther culture on flue-cured lines of tobacco. Crop Sci. 23: 729–733.

    Article  Google Scholar 

  • Burk, L.G. and D.F. Matzinger, 1976. Variation among anther-derived doubled haploids from an inbred line of tobacco. J. Hered. 67: 381–384.

    Google Scholar 

  • Burk, L.G., J.F. Chaplin, G.V. Gooding and N.T. Powell, 1979a. Quantity production of anther-derived haploids from a multiple disease resistant tobacco hybrid. I. Frequency of plants with resistance or susceptibility to TMV, potato virus Y, and root knot ( RK ). Euphytica 28: 201–208.

    Google Scholar 

  • Burk, L.G., D.U. Gerstel and E.A. Wernsman, 1979b. Maternal haploids of Nicotiana tabacum L. from seed. Science 206: 585.

    Article  PubMed  CAS  Google Scholar 

  • Burk, L.G., G.R. Gwynn and J.F. Chaplin, 1972. Diploidized haploids from aseptically cultured anthers of Nicotiana tabacum. J. Hered. 63: 355–360.

    Google Scholar 

  • Collins, G.B. and P.D. Legg, 1974. The use of haploids in breeding allopolyploid species. In: K.J. Kasha (Ed.), Haploids in Higher Plants. Advances and Potential, Proc. First Intl. Symp., pp. 231–274. University of Guelph, Guelph, Ontario.

    Google Scholar 

  • Collins, G.B. and P.D. Legg, 1980. Recent advances in the genetic application of haploidy in Nicotiana. In: D.R. Davies and D.A. Hopwood (Eds.), The Plant Genome, pp. 197–213. John Innes Institute, Norwich.

    Google Scholar 

  • Collins, G.B., P.D. Legg and C.C. Litton, 1974. The use of anther derived haploids in Nicotiana. II. Comparison of doubled haploid lines with lines obtained by conventional breeding methods. Tobacco Sci. 18: 40–42.

    Google Scholar 

  • D’Amato, F., M. Devreux and G.T. Scarscia Mugnozza, 1965. The DNA content of the nuclei of the pollen grains in tobacco and barley. Caryologia 18: 377–382.

    Google Scholar 

  • Deaton, W.R., P.D. Legg and G.B. Collins, 1982. A comparison of burley tobacco doubled-haploid lines with their source inbred cultivars. Theor. Appl. Genet. 62: 69–74.

    Google Scholar 

  • Deaton, W.R., G.B. Collins and M.T. Nielsen, 1986a. Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.). I. Comparison of sexual and doubled-haploid populations. Euphytica 35: 33–40.

    Google Scholar 

  • Deaton, W.R., G.B. Collins and M.T. Nielsen, 1986b. Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.). II. Evaluation of first-and second-cycle doubled haploids. Euphytica 35: 41–48.

    Article  Google Scholar 

  • De Paepe, R., D. Bleton and F. Gnangbe, 1981. Basis and extent of genetic variability among doubled haploid plants obtained by pollen culture in Nicotiana sylvestris. Theor. Appl. Genet. 59: 177–184.

    Google Scholar 

  • De Paepe, R., A. Koulou, J.L. Pham and S.C. Brown, 1990. Nuclear DNA content and separation of Nicotiana sylvestris vegetative and generative nuclei at various stages of male gametogenesis. Plant Sci. 70: 255–265.

    Article  Google Scholar 

  • De Paepe, R., D. Prat and T. Huguet, 1982. Heritable nuclear DNA changes in doubled haploid plants obtained by pollen culture of Nicotiana sylvestris. Plant Sci. Lett. 28: 11–28.

    Google Scholar 

  • Devonshire, A.L. and G.D. Moores, 1982. A carboxylesterase with broad substrate specificity causes organophosphorus, carbamate, and pyrethroid resistance in peach-potato aphids (Myzus persicae). Pestic. Biochem. Physiol. 18: 235–246.

    Google Scholar 

  • Dhillon, S.S., E.A. Wernsman and J.P. Miksche, 1983. Evaluation of nuclear DNA content and heterochromatin changes in anther-derived dihaploids of tobacco (Nicotiana tabacum) cv. Coker 139. Can. J. Genet. Cytol. 25: 169–173.

    Google Scholar 

  • Donn, G., E. Tischer, J.A. Smith and H.M. Goodman, 1984. Herbicide-resistant alfalfa cells: an example of gene amplification in plants. J. Mol. Appl. Genet. 2: 621–635.

    Google Scholar 

  • Evans, D.A., W.R. Sharp and H.P. Medino-Filho, 1984. Somaclonal and gametoclonal variation. Amer. J. Bot. 71: 759–774.

    Google Scholar 

  • Fallon, A.M. 1984. Methotrexate resistance in cultured mosquito cells. Insect. Biochem. 14: 697–704.

    Google Scholar 

  • Gerstel, D.U., J.A. Burns and L.G. Burk, 1974. Meiosis in F1 hybrids between haploid-derived diploids and their parents. Tobacco Sci. 18: 122–124.

    Google Scholar 

  • Goldsbrough, P.B., E.M. Hatch, B. Huang, W.G. Kosinski, W.E. Dyer, K.M. Herrmann and S.C. Weller, 1990. Gene amplification in glyphosate tolerant tobacco cells. Plant Sci. 72: 5362.

    Article  Google Scholar 

  • Kasperbauer, M.J. and G.B. Collins, 1972. Reconstitution of diploids from leaf tissue of anther-derived haploids in tobacco. Crop Sci. 12: 98–101.

    Article  Google Scholar 

  • Kasperbauer, M.J., P.D. Legg and T.G. Sutton, 1983. Growth, development, and alkaloid content of doubled haploids vs. inbreds of burley tobacco. Crop Sci. 23: 965–969.

    Article  CAS  Google Scholar 

  • Kumashiro, T. and T. Oinuma, 1985. Comparison of genetic variability among anther-derived and ovule-derived doubled haploid lines of tobacco. Jpn. J. Breed. 35: 301–310.

    Google Scholar 

  • Kumashiro, T. and T. Oinuma, 1986. Genetic variation of colchicine induced and spontaneous doubled haploids obtained by anther culture of an inbred tobacco. Jpn. J. Breed. 36: 355–362.

    Google Scholar 

  • Matzinger, D.F. and L.G. Burk, 1984. Cytoplasmic modification by anther culture in Nicotiana tabacum L. J. Hered. 75: 167–170.

    Google Scholar 

  • Mouches, C., N. Pasteur, J.B. Berge, O. Hyrien, M. Raymond, B.R. de Saint Vincent, M. de Silvestri and G.P. Geroghiou, 1986. Amplification of an esterase gene is responsible for insecticide resistance in a California Culex mosquito. Science 233: 778–780.

    Article  PubMed  CAS  Google Scholar 

  • Nakata, K. and T. Kurihara, 1972. Competition among pollen grains for haploid tobacco plant formation by anther culture. II. Analysis with resistance to tobacco mosaic virus (TMV) and wildfire disease, leaf color and leaf base shape characteristics. Jpn. J. Breed. 22: 92–98.

    Google Scholar 

  • Nielsen, M.T. and G.B. Collins, 1989. Variation among androgenic and gynogenic doubled haploids of tobacco (Nicotiana tabacum L.). Euphytica 43: 263–267.

    Article  Google Scholar 

  • Oinuma, T. and T. Yoshida, 1974. Genetic variation among doubled haploid lines of burley tobacco varieties. Jpn. J. Breed. 24: 211–216.

    Google Scholar 

  • Reed, S.M. and J.A. Burns, 1989. Cytological consequences of DNA amplification in an anther-derived doubled haploid line of Nicotiana tabacum L. Amer. J. Bot. 76: 958–965.

    Google Scholar 

  • Reed, S.M. and E.A. Wernsman, 1989. DNA amplification among anther-derived doubled haploid lines of tobacco and its relationship to agronomic performance. Crop Sci. 29: 1072–1076.

    Article  Google Scholar 

  • Reed, S.M., J.A. Burns and E.A. Wernsman, 1992. Cytological comparison of amplified chromosome segments in four tobacco doubled haploids. Crop Sci. 32: 362–366.

    Article  Google Scholar 

  • Reed, S.M., J.A. Burns and E.A. Wernsman, 1994. Cytological evaluation of tobacco doubled haploids for presence of amplified homologous chromosomal regions. Crop Sci. 34: 236–239.

    Article  Google Scholar 

  • Reed, S.M., E.A. Wernsman and J.A Burns, 1991. Aberrant cytological behavior in tobacco androgenic doubled haploid x parental cultivar hybrids. Crop Sci. 31: 97–101.

    Article  Google Scholar 

  • Schimke, R.T., 1984. Gene amplification in cultured animal cells. Cell 37: 705–713.

    Article  PubMed  CAS  Google Scholar 

  • Schnell, R.J. II, E.A. Wernsman and L.G. Burk, 1980. Efficiency of single-seed-descent vs. anther-derived dihaploid breeding methods in tobacco. Crop Sci. 20: 619–622.

    Article  Google Scholar 

  • Shyr, Y.-Y.J., A.G. Hepburn and J.M. Widholm, 1992. Glyphosate selected amplification of the 5-enolpyruvylshikimate-3-phosphate synthase gene in cultured carrot cells. Mol. Gen. Genet. 232: 377–382.

    Google Scholar 

  • Sunderland, N. and F.M. Wicks, 1971. Embryoid formation in pollen grains of Nicotiana tabacum. J. Exp. Bot. 22: 213–226.

    Google Scholar 

  • Vagera, J., F.J. Novak and B. Vyskot, 1976. Anther cultures of Nicotiana tabacum L. mutants. Theor. Appl. Genet. 47: 109–114.

    Google Scholar 

  • Wernsman, E.A., D.F. Matzinger and R.C. Rufty, 1989. Androgenetic vs. gynogenetic doubled haploids of tobacco. Crop Sci. 29: 1151–1155.

    Article  Google Scholar 

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Authors
  1. Sandra M. Reed
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Editors and Affiliations

  1. Plant Production Department, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. School of Life Science, Jawaharlal Nehru University, New Delhi, India

    S. K. Sopory

  3. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    R. E. Veilleux

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© 1996 Springer Science+Business Media Dordrecht

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Reed, S.M. (1996). DNA amplification in doubled haploids of Nicotiana . In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0477-9_5

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  • DOI: https://doi.org/10.1007/978-94-017-0477-9_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4580-5

  • Online ISBN: 978-94-017-0477-9

  • eBook Packages: Springer Book Archive

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