Abstract
Almost all gene transfer techniques except pollen-based methods require a plant regeneration system. Whether in vitro steps must be included in pollen-mediated transfer depends on the selectable marker genes, e.g., in vitro culture will not be required using anthocyanin markers (Hess, 1980). As another example, the hybrid Nicotiana glauca Ć N. langsdorffii is well-known for the formation of genetic tumors. To transfer the tumor gene āIā from N. langsdorffii to N. glauca via pollen, N. glauca pollen cultures were treated with N. langsdorffii DNA, and pollen-derived plants were grown in soil. Tumor phenotype was screened by wounding the plants. Again, in vitro steps could be avoided by this approach (Hess, 1975; Hess et al., 1976).
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Hess, D. (1996). Genetic transformation of wheat via pollen 25 Years of plant transformation attempts II . In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0477-9_20
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