Abstract
Commercial pectinases used in apple juice manufacturing contain a mixture of pectin-degrading enzyme activities. Nevertheless, fragments of branched pectic molecules (or pectic hairy regions) are resistant to degradation, and can cause membrane fouling in the final ultrafiltration step of concentrated apple juice. The pectic hairy regions contain both rhamno- and xylogalacturonan. Whereas rhamnogalacturonan-degrading enzymes were already identified, no enzyme was known for a fast degradation of xylogalacturonan. Apparently, these enzymes are very minor components of technical pectinases. Since Aspergillus strains are able to completely degrade pectin, including xylogalacturonan, we decided to use expression cloning to obtain xylogalacturonan-degrading enzymes.
A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple.
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© 2003 Springer Science+Business Media Dordrecht
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Herweijer, M.A. et al. (2003). Endo-Xylogalacturonan Hydrolase. In: Voragen, F., Schols, H., Visser, R. (eds) Advances in Pectin and Pectinase Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0331-4_19
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DOI: https://doi.org/10.1007/978-94-017-0331-4_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6229-1
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