Abstract
Biotechnology, a multidisciplinary science, has many applications. A major application in chemical and pharmaceutical industries involves the use of enzymes, mostly from microbial sources, in the production process of many useful products. Various compounds including many proteins and enzymes are produced through microbial fermentation. Filamentous fungi, with their many virtues and long history of industrial use, have lately become targets for gene manipulation for producing strains with improved yield and better expression-secretion system for homologous and heterologous eukaryotic gene products. To genetically modify filamentous fungi for enhanced expression of a desired gene and secretion of its product, it requires a suitably designed gene-construct with correctly chosen and aligned sequences of the gene of interest and its controlling elements, as well as an efficient gene-transfer system for transforming the host fungus. An important feature of the transformation of filamentous fungi is that the transforming DNA stably integrates into the host genome. Substantial research for understanding the molecular aspects of filamentous fungal expression systems is necessary for its biotechnological application in an industrial context.
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Banerjee, A.C., Kundu, A., Ghosh, S.K. (2003). Genetic Manipulation of Filamentous Fungi. In: Roussos, S., Soccol, C.R., Pandey, A., Augur, C. (eds) New Horizons in Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0203-4_17
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DOI: https://doi.org/10.1007/978-94-017-0203-4_17
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