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Enhanced Loading and Stability of Urease Covalently Attached to Polypyrrole Microspheres for Application to Urea Biosensor

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New Horizons in Biotechnology

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Abstract

An electrode for measurement of urea, based on urease covalently attached to polypyrrole (PPY) microspheres immobilized on conducting polypyrrole polyvinyl sulphonate (PPY-PVS) films has been described. Conducting polypyrrole polyvinyl sulphonate films were prepared using indium-tin-oxide (ITO) glass plates as working electrode and standard calomel electrode as the reference electrode. The films were deposited on ITO glass plates for 5–7 minutes at a constant current of 2mA (surface area 1x1cm2). Urease immobilized onto polypyrrole microspheres (by reaction of protein amino groups with microsphere surface aldehyde groups of the surface of microspheres) was entrapped/adsorbed on electrochemically prepared conducting polypyrrole polyvinyl sulphonate films deposited on ITO. Potentiometric measurements were taken in different concentrations of urea using an ammonium ion analyser.

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Author information

Authors and Affiliations

  1. Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, K. S. Krishnan Marg, New Delhi, 110012, India

    A. Gambhir, A. Kumar & B. D. Malhotra

  2. Center of Molecular and Macromolecular Studies Sienkiewicza 112, 90-363, Lodz, Poland

    B. Miksa & S. Slomkowski

Authors
  1. A. Gambhir
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  2. A. Kumar
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  3. B. D. Malhotra
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  4. B. Miksa
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  5. S. Slomkowski
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Editor information

Editors and Affiliations

  1. Laboratoire de Microbiologie IRD, IFR-BAIM, Université de Provence, Marseille, France

    S. Roussos  & C. Augur  & 

  2. Laboratorio de Processos Biotecnologicos, Departamento Engenharia Quimica, Universidade Federal do Parana (UFPR), Curitiba, PR, Brazil

    C. R. Soccol

  3. Biotechnology Division, Regional Research Laboratories, CSIR, Trivandrum, India

    A. Pandey

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© 2003 Springer Science+Business Media Dordrecht

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Gambhir, A., Kumar, A., Malhotra, B.D., Miksa, B., Slomkowski, S. (2003). Enhanced Loading and Stability of Urease Covalently Attached to Polypyrrole Microspheres for Application to Urea Biosensor. In: Roussos, S., Soccol, C.R., Pandey, A., Augur, C. (eds) New Horizons in Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0203-4_14

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  • DOI: https://doi.org/10.1007/978-94-017-0203-4_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6468-4

  • Online ISBN: 978-94-017-0203-4

  • eBook Packages: Springer Book Archive

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