Abstract
Steryl esters and triacylglycerol (TAG) are the main storage lipids in eukaryotic cells. In the yeast Saccharomyces cerevisiae, these storage lipids accumulate during stationary growth phase within organelles known as lipid bodies. We have used multiple gene disruptions to study storage lipid biosynthesis in yeast. Four genes, ARE1, ARE2, DGA1 and LRO1, were found to contribute to TAG synthesis. A yeast strain that lacks all four genes is viable and has no apparent growth defects under standard conditions. The strain is devoid of both TAG and steryl esters, and fluorescence microscopy revealed that it also lacks lipid bodies [1]. We have now further investigated the lipid content and found differences between the two strains. The levels of diacylglycerol and fatty acids as well as the composition of acyl groups were altered in the disrupted strain compared to the wild type. We conclude that even though storage lipids are non-essential for growth in yeast the lack of synthesis of storage lipids affects the acyl group composition of different lipid groups.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
I. Sandager, L., Gustaysson, M. H., Stahl, U., Dahlqvist, A., Wiberg, E., Banas, A., Lenman, M., Ronne, H., and Stymne, S. (2002) Storage lipid synthesis is non-essential in yeast. J Biol Chem 277, 6478–82
Sandager, L., Dahlqvist, A., Banas, A., Stahl, U., Lenman, M., Gustaysson, M., and Stymne, S. (2000) An acyl-CoA:cholesterol acyltransferase (ACAT)-related gene is involved in the accumulation of triacylglycerol in Saccharomyces cerevisiae. Biochem Soc Trans 28, 700–2.
Yu, C., Kennedy, N. J., Chang, C. C., and Rothblatt, J. A. (1996) Molecular cloning and characterization of two isoforms of Saccharomyces cerevisiae acyl-CoA:sterol acyltransferase. J Biol Chem 271, 24157–63.
Zweytick, D., Leitner, E., Kohlwein, S. D., Yu, C., Rothblatt, J., and Daum, G. (2000) Contribution of Arelp and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiae. Eur J Biochem 267, 107–582.
Dahlqvist, A., Stahl, U., Lenman, M., Banas, A., Lee, M., Sandager, L., Ronne, H., and Stymne, S. (2000) Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci U S A 97, 6487–92.
Schneiter, R., and Kohlwein, S. D. (1997) Organelle structure, function, and inheritance in yeast: a role for fatty acid synthesis? Cell 88, 431–4.
Taylor, F. R., and Parks, L. W. (1979) Triaglycerol metabolism in Saccharomyces cerevisiae. Relation to phospholipid synthesis. Biochim Biophys Acta 575, 204–14.
Thomas, B. J., and Rothstein, R. (1989) Elevated recombination rates in transcriptionally active DNA. Cell 56, 619–30.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Gustavsson, M.H., Sandager, L., Dahlqvist, A., Ronne, H., Stymne, S. (2003). Lipid Composition in Yeast Mutant Lacking Storage Lipids Synthesis Capacity. In: Murata, N., Yamada, M., Nishida, I., Okuyama, H., Sekiya, J., Hajime, W. (eds) Advanced Research on Plant Lipids. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0159-4_37
Download citation
DOI: https://doi.org/10.1007/978-94-017-0159-4_37
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6210-9
Online ISBN: 978-94-017-0159-4
eBook Packages: Springer Book Archive