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In Vitro Regeneration and Genetic Transformation of Vicia Species

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Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

Abstract

Vicia species have been subjected to cell and tissue culture experiments for approximately 40 years. The economically most important species of this genus, the faba-bean, continuously exhibited (like many other grain legumes) a rather low amenability to in vitro conditions. Not only the low capability to regenerate shoots or somatic embryos from dedifferentiated tissue, but also the fact that expiants and callus cells of Vicia faba tend to produce high amounts of phenolic compounds resulting in subsequent mortifying of the tissue, has strongly limited the application of genetic engineering techniques in this species. As a consequence, among the major grain legume crops Vicia faba was the last species where the production of transgenic plants has (only recently) been reported. In contrast, the closely related species Vicia narbonensis could relatively easily be manipulated in vitro. In this chapter, an overview on tissue culture and genetic manipulation of Vicia species has been described.

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  1. Institute for Applied Genetics, Free University of Berlin, Albrecht-Thaer-Weg 6, 14195, Berlin, Germany

    Thomas Pickardt

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  1. Thomas Pickardt
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  1. Department of Biosciences, M.D. University, Rohtak, 124001, India

    Pawan K. Jaiwal  & Rana P. Singh  & 

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Pickardt, T. (2003). In Vitro Regeneration and Genetic Transformation of Vicia Species. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_7

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  • DOI: https://doi.org/10.1007/978-94-017-0139-6_7

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