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Genetic Transformation of Common Bean Via Particle Bombardment

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Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

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Abstract

Success in bean (Phaseolus vulgaris L.) transformation requires an understanding of both tissue culture and transformations systems. Several techniques have been proposed in order to develop an efficient technology to transform bean. The susceptibility of bean to Agrobacterium has been demonstrated and electroporation or polyethylenglycol (PEG)-mediated transformation has been evaluated. Using the particle bombardment process, transgenic bean plants containing agronomically important traits from several varieties have been obtained. Some aspects influencing the achievement of transgenic P. vulgaris by particle bombardment, limitations and future prospects are discussed.

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Authors and Affiliations

  1. Embrapa Recursos Genéticos e Biotecnología, Laboratório de Introdução e Expressão de Genes, SAIN Parque Rural, C.P.O. 2372, Brasilía, DF, 70770-900, Brazil

    Francisco J. L. Aragäo

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  1. Francisco J. L. Aragäo
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  1. Department of Biosciences, M.D. University, Rohtak, 124001, India

    Pawan K. Jaiwal  & Rana P. Singh  & 

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Aragäo, F.J.L. (2003). Genetic Transformation of Common Bean Via Particle Bombardment. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_3

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  • DOI: https://doi.org/10.1007/978-94-017-0139-6_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6369-4

  • Online ISBN: 978-94-017-0139-6

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