Abstract
Due to rapid deforestation, depletion of genetic stocks, and escalating product demands, there has been a sharp decline in the forest cover globally. Thus, mass propagation and production of short duration trees with a rapid biomass turnover is the need of the present times. Tissue culture and genetic transformation techniques are envisaged to play a pivotal role in meeting this increasing demand. The legumes are not only important nitrogen fixers, providing protein and vitamin-rich food and fodder, but also include appealing ornamentals and high valued timber species. The present article focuses on the state of knowledge in the leguminous tree species with respect to their amenability to in vitro practices and highlights the genetic engineering attempts in this important group of plants. Albizzia lebbeck is taken as a model leguminous tree species, as not only organogenesis but even somatic embryogenesis has been reported in this plant species. Molecular investigations have been carried out to reveal the role of various physical and chemical factors affecting somatic embryogenesis. Attempts towards genetically manipulating Albizzia, both by Agrobacterium-mediated gene transfer and biolistic-mediated gene delivery, are discussed in the present chapter.
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Khurana, P., Khurana, J., Jani, M. (2003). Regeneration and Genetic Transformation of Tree Legumes with Special Reference to Albizzia Species. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_18
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