Skip to main content

Regeneration and Genetic Transformation of Tree Legumes with Special Reference to Albizzia Species

  • Chapter
Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

  • 236 Accesses

Abstract

Due to rapid deforestation, depletion of genetic stocks, and escalating product demands, there has been a sharp decline in the forest cover globally. Thus, mass propagation and production of short duration trees with a rapid biomass turnover is the need of the present times. Tissue culture and genetic transformation techniques are envisaged to play a pivotal role in meeting this increasing demand. The legumes are not only important nitrogen fixers, providing protein and vitamin-rich food and fodder, but also include appealing ornamentals and high valued timber species. The present article focuses on the state of knowledge in the leguminous tree species with respect to their amenability to in vitro practices and highlights the genetic engineering attempts in this important group of plants. Albizzia lebbeck is taken as a model leguminous tree species, as not only organogenesis but even somatic embryogenesis has been reported in this plant species. Molecular investigations have been carried out to reveal the role of various physical and chemical factors affecting somatic embryogenesis. Attempts towards genetically manipulating Albizzia, both by Agrobacterium-mediated gene transfer and biolistic-mediated gene delivery, are discussed in the present chapter.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Anand M and Bir S S (1984) Organogenetic differentiation in tissue cultures of Dalbergia lanceolaria. Curr. Sci., 53: 1305–1307.

    Google Scholar 

  • Angenon G and Montagu V M (1992) Transgenic plants: Agrobacterium mediated transformation and its application in plant molecular biology research and biotechnology. In: Biotechnology and Crop Improvement in Asia (Ed Moss JP), pp. 181–199.

    Google Scholar 

  • Anonymous (1985) In: Wealth of India, CSIR, New Delhi, 5, 6–8.

    Google Scholar 

  • Arndt F, Rusch R and Stilfried H V (1976) SN 49537, a new cotton defoliant. Plant Physiol, 57: 99.

    Google Scholar 

  • Arrillaga I, Tobolski J J and Merkle S A (1994) Advances in somatic embryogenesis and plant production of black locust (Robiniapseudoacacia L.). Plant Cell Rep., 13: 171–175.

    CAS  Google Scholar 

  • Arya H C (1984) Tissue culture — an aid to forestry with special reference to Rajasthan. In: National Seminar, Application of Science and Technology for Afforestation, Jaipur, India, 60–63.

    Google Scholar 

  • Badji S, Mairone Y, Ndiaye I, Merlin G, Danthu P, Neville P and Colonna J P (1992) In vitro propagation of gum Arabic tree (Acacia Senegal (L.) Wild). Developing a rapid method for producing plants. Plant Cell Rep., 12: 629–633.

    Google Scholar 

  • Bajaj Y P S and Dhanju M S (1983) Pollen embryogenesis in three ornamental trees Cassia fistula, Jacaranda acutifolia and Poinciana regia. J. Tree Sci., 2: 16–19.

    Google Scholar 

  • Barghchi M (1987) Mass clonal propagation in vitro of Robinia pseudoacacia (Black Locust) cv. Jaszkiseri. Plant Sci., 53: 435–45.

    Article  Google Scholar 

  • Baweja K, Khurana J P and Gharyal-Khurana P (1995) Influence of light on somatic embryogenesis in hypocotyls of Albizzia lebbeck. Curr. Sci., 68: 544–546.

    Google Scholar 

  • Berger K, Schaffner W (1995). In vitro propagation of the leguminous tree Swartzia madagascariensis. Plant Cell Tiss. Org. Cult, 40: 289–291.

    Article  Google Scholar 

  • Biddington N L (1992) The influence of ethylene in plant tissue culture. Plant Growth Reg., 11: 173–187.

    Article  CAS  Google Scholar 

  • Bonga J M (1977) Applications of tissue culture in forestry. In: Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture (Eds Reinert J and Bajaj Y P S), Berlin-Heidelberg-New York, Springer, 93–108.

    Google Scholar 

  • Cabrera-Ponce J L, Vegas-Garcia A and Herrera-Estrella L (1994) Herbicide resistant transgenic papaya produced by an efficient particle bombardment transformation method. Plant Cell Rep., 15: 1–7.

    Article  Google Scholar 

  • Chalupa V (1983) In vitro propagation of willows (Salix spp.), European mountain Ash (Sorbus aucuparia) and Black Locust (Robiniapseudoacacia L.). Biol. Plant., 25: 305–307.

    Article  Google Scholar 

  • Chalupa V (1992) Tissue culture propagation of black locust. In: Black Locust: Biology, Culture and Utilization. Proc. Int. Conf. Black Locust (Eds Hanover J W, Miller K and Plesko S), East Lansing, MI, USA, 115–125.

    Google Scholar 

  • Das A B, Rout G R and Das P (1995) In vitro somatic embryogenesis from callus cultures of the timber yielding tree Hardwickia binata Roxb. Plant Cell Rep., 15: 147–149.

    Article  CAS  Google Scholar 

  • Das P, Roberts A V and Rout G R (1997) In vitro somatic embryogenesis in Dalbergia sissoo Roxb. — a multipurpose timber-yielding tree. Plant Cell Rep., 16: 578–582.

    CAS  Google Scholar 

  • Datta S K, Datta K and Pramanik T (1983) In vitro clonal multiplication of mature trees of Dalbergia sissoo Roxb. Plant Cell Tiss. Org. Cult., 2: 15–20.

    Article  Google Scholar 

  • Davis J M and Keathley D E (1985) Regeneration of shoots from leaf disk expiants of black locust, Robinia pseudoacacia L. In: Proc 4th North Central Tree Improvement Conf., East Lansing, MI, USA, 29–34.

    Google Scholar 

  • Davis J M and Keathley D E (1987) Differential responses to in vitro bud culture in mature Robinia pseudoacacia L (black locust). Plant Cell Rep., 6: 431–434.

    CAS  Google Scholar 

  • De Cleene M and De Ley J (1976) The host range of crown gall. Bot. Rev., 42: 389–466.

    Article  Google Scholar 

  • Debergh P, Aitken-Christie J and Cohen D (1992) Reconsideration of the term vitrification as used in micro-propagation. Plant Cell Tiss. Org. Cult., 30: 135–140.

    Article  Google Scholar 

  • Detrez C, Ndiaye S and Dreyfus B (1994) In vitro regeneration of the tropical multipurpose leguminous tree Sesbania grandiflora from cotyledon expiants. Plant Cell Rep., 14: 87–93.

    CAS  Google Scholar 

  • Dewan A, Nanda K and Gupta S C (1992). In vitro micropropagation of Acacia nilotica subsp. Indica Brenan via cotyledonary nodes. Plant Cell Rep., 12: 18–21.

    Article  Google Scholar 

  • Duhoux E and Davies D (1985) Caulogenese a partir des bourgeons cotyledonaires & Acacia albida et influence du saccharose sur la rhyzogenese. J. Plant Physiol., 121: 175–180.

    Article  CAS  Google Scholar 

  • Evans J M and Batty N P (1994) Ethylene precursors and antagonists increase embryogenesis in Hordeum vulgare Viv. Anther culture. Plant Cell Rep., 13: 678–679.

    Article  Google Scholar 

  • Fasolo F, Zimmerman R H and Fordham H (1989) Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant Cell Tiss. Org. Cult., 16: 75–79.

    Article  CAS  Google Scholar 

  • Fitch M M M, Manshardt R M, Gonsalves D, Slightom J L and Sanford J C (1990) Stable transformation of papaya via microprojectile bombardment. Plant Cell Rep., 9: 189–194.

    CAS  Google Scholar 

  • Fluhr R and Mattoo A K (1996) Ethylene biosynthesis and perception. Cht. Rev. Plant Sci., 15: 479–523.

    CAS  Google Scholar 

  • Gamborg O L, Miller R A and Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Expt. Cell Res., 50: 151–158.

    Article  CAS  Google Scholar 

  • Garg L, Bhandari N, Rani V and Bhojwani S S (1995) Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica. Plant Cell Rep., 15: 855–858.

    Article  Google Scholar 

  • Gharyal P K (1983) In vitro differentiation in some leguminous species. Ph.D. Thesis, University of Delhi, Delhi, India.

    Google Scholar 

  • Gharyal P K and Maheshwari S C (1981) In vitro differentiation of somatic embryoids in a leguminous tree, Albizzia lebbeck L. Naturwissenchaften, 68: 379–380.

    Article  Google Scholar 

  • Gharyal P K and Maheshwari S C (1983) In vitro differentiation of plantlets from tissue cultures of Albizzia lebbeck L. Plant Cell Tiss. Org. Cult., 2: 49–53.

    Article  Google Scholar 

  • Gharyal P K and Maheshwari S C (1990) Differentiation in expiants from mature leguminous trees. Plant Cell Rep., 8: 550–553.

    Article  Google Scholar 

  • Gharyal P K, Baweja K and Maheshwari S C (1992) Albizzia lebbeck: a model system for studying differentiation. In: Biotechnology for Forest Tree Improvement, BIOTROP Special Pub. No. 49, Southeast Asian Regional Centre for Tropical Biology, Bogor, Indonesia, 141–148.

    Google Scholar 

  • Gharyal P K, Rashid A and Maheshwari S C (1983a) Production of haploid plantlets in anther cultures of Albizzia lebbeckL. Plant Cell Rep., 2: 308–309.

    Article  CAS  Google Scholar 

  • Gharyal P K, Rashid A and Maheshwari S C (1983b) Androgenic response from cultured anthers of a leguminous tree, Cassia siamea L. Protoplasma, 118: 91–93.

    Article  Google Scholar 

  • Green B, Tabone T and Felker P (1990) A comparison of amide and ureide nitrogen sources in tissue culture of tree legume Prosopis alba clone B2 V50. Plant Cell Tiss. Org. Cult., 21: 83–86.

    Article  CAS  Google Scholar 

  • Guha S and Maheshwari S C (1966) Cell division and differentiation of embryos in Datura in vitro. Nature, 212: 97–98.

    Article  Google Scholar 

  • Gupta N, Jain S K and Srivastava P S (1996) In vitro micropropagation of a multipurpose leguminous tree — Delonix regia. Phytomorphology, 46: 267–275.

    Google Scholar 

  • Han K H and Keathley D E (1989) Regeneration of whole plants from seedling-derived callus of black locust (Robinia pseudoacacia L.). Nitrogen Fixing Tree Res. Rep., 7: 112–114.

    Google Scholar 

  • Han K H, Keathley D E, Davis J M and Gordon M P (1991) Regeneration of transgenic woody legume (Robinia pseudoacacia) and morphological alterations induced by Agrobacterium rhizogenes mediated transformation. Plant Sci., 88: 149–157.

    Article  Google Scholar 

  • Han K H, Gordon M P and Keathley D E (1999) Genetic transformation of black locust (Robinia pseudoacacia L.). In: Biotechnology in Agriculture and Forestry, Vol 44, Transgenic Trees (Ed. Bajaj Y P S), Springer-Verlag, Berlin and Heidelberg, 273–282.

    Google Scholar 

  • Han K H, Keathley D E and Milton P G (1993) Cambial tissue culture and subsequent shoot regeneration from mature black locust (Robiniapseudoacacia L.) Plant Cell Rep., 12: 185–188.

    Article  Google Scholar 

  • Igasaki T, Mohri T, Ichikawa H and Shinohara K (2000) Agrob acter ium tumefaciens-mediated transformation of Robinia pseudoacacia. Plant Cell Rep., 19: 448–453.

    Article  CAS  Google Scholar 

  • Jaiwal P K and Gulati A (1991) In vitro high frequency plant regeneration of a tree legume Tamarindus indica L. Plant Cell Rep., 10: 569–573.

    Article  CAS  Google Scholar 

  • Jordan M (1987) In vitro culture of Prosopis species. In: Cell and Tissue Culture in Forestry. Vol 3 (Eds Bonga J M and Durzan D J), Martinus Nijhoff Publications, Boston, MA, 370–384.

    Chapter  Google Scholar 

  • Kackar N L, Vyas S C, Singh M and Solanki K R (1992) In vitro regeneration of Prosopis cineraria (L.) Druce using root as expiant. Indian J. Exp. Biol., 30: 429–430.

    Google Scholar 

  • Kaur K, Verma B and Kant U (1998) Plants obtained from the Khair tree (Acacia catechu Willd.) using mature nodal segments. Plant Cell Rep., 17: 427–429.

    Article  CAS  Google Scholar 

  • Khurana J (1998) Prospects for genetic manipulation of tree species (Part I) and in vitro differentiation and transformation studies on the leguminous tree — Albizzia Lebbeck L. (Part II), M.Sc. Thesis, University of Delhi, Delhi, India.

    Google Scholar 

  • Khurana J and Khurana P K (2000) Biolistic-mediated DNA delivery into hypocotyls of a leguminous tree — Albizzia lebbeck L.: influence of biological and physical parameters. J. Plant Biochem. and Biotech., 9: 31–34.

    Article  CAS  Google Scholar 

  • Khurana P and Khurana J (1999) Applications of genetic transformation to tree biotechnology. Indian J. Exp. Biol, 37: 627–638.

    Google Scholar 

  • Klein T M, Wolf E D, Wu R and Sanford J C (1987) High velocity microprojectiles for delivery of nucleic acids into living cells. Nature, 327: 70–73.

    Article  CAS  Google Scholar 

  • Kumar A (1993) Micropropagation of a mature leguminous tree — Bauhinia purpurea. Plant Cell Tiss. Org. Cult., 31: 257–259.

    Google Scholar 

  • Kumar A, Tandon P and Sharma A (1991) Morphogenetic responses of cultured cells of cambial origin of mature tree Dalbergia sissoo Roxb. Plant Cell Rep., 9: 703–705.

    Article  Google Scholar 

  • Kumar S, Sarkar A K and Kunhikannan C (1998) Regeneration of plants from leaflet expiants of tissue culture raised safed siris (Albizziaprocera). Plant Cell Tiss. Org. Cult., 54: 137–143.

    Article  CAS  Google Scholar 

  • Lakshmi Sita G, Chattopadhyay S and Tejawathi D M (1986) Plant regeneration from shoot callus of rosewood. (Dalbergia lati/olia Roxb.) Plant Cell Rep., 5: 266–268.

    Article  Google Scholar 

  • Lakshmi Sita G and Raghavaswamy B V (1993) Regeneration of plantlets from leaf disc cultures of rosewood: control of leaf abscission and shoot tip necrosis. Plant Sci., 88: 107–110.

    Article  CAS  Google Scholar 

  • Lee S K and Rao A N (1980) Tissue culture of certain tropical trees. In: Plant Cell Culture: Results and Prospectives (Eds Sala F, Parisi B, Cella R and Cifferri O), Elsevier/North-Holland, Amsterdam and New York, 305–311.

    Google Scholar 

  • Maheshwari S C, Gill R, Maheshwari N and Gharyal P K (1996) Isolation and regeneration of protoplasts from higher plants. In: Differentiation of Protoplasts and of Transformed Plant Cells (Eds Reinert J and Binding H), Springer-Verlag, Berlin, 3–36.

    Google Scholar 

  • Martins-Loucao M A (1990) Carob (Ceratonia siliqua L). In: Biotechnology in Agriculture and Forestry. Vol 10, Legumes and Oilseed Crops I (Ed. Bajaj Y P S), Springer-Verlag, Berlin and Heidelberg, 658–667.

    Chapter  Google Scholar 

  • Mathur J and Mukuntha Kumar S (1992). Micropropagation of Bauhinia variegata and Parkinsonia aculeate from nodal expiants of mature trees. Plant Cell Tiss. Org. Cult., 28: 119–121.

    Article  CAS  Google Scholar 

  • McCown D, McCabe D E, Russell D R, Robison D J, Barton K A and Raffa K F (1990) Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration. Plant Cell Rep., 9: 590–594.

    Google Scholar 

  • Merkle S A, and Wiecko A T (1989) Regeneration of Robinia pseudoacacia via somatic embryogenesis. Can. J. For. Res., 19: 285–288.

    Article  Google Scholar 

  • Meyer M M and Kerns H R (1986) TDZ and in vitro shoot proliferation oîCeltis occidentalis L. In: Proc VI Intl. Congr Plant Tissue and Cell Culture, Minneapolis, Abstract, 149.

    Google Scholar 

  • Miller C O (1961) Kinetin related compounds in plant growth. Annu. Rev. Plant Physiol, 12: 395–408.

    Article  CAS  Google Scholar 

  • Mukhopadhyay A and Mohan Ram H Y (1986) Regeneration of plantlets from excised roots of Dalbergia sissoo. Indian J. Exp. Biol, 19: 1113–1115.

    Google Scholar 

  • Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473–497.

    Article  CAS  Google Scholar 

  • Nandwani D and Ramawat K G (1992) High frequency plantlet regeneration from seedling expiants of Prosopis tamarugo. Plant Cell Tiss. Org. Cult., 29: 56–60.

    Article  Google Scholar 

  • Newman M C, Preece J E, Van Sambeek J W and Gaffney G R (1993) Somatic embryogenesis and callus production from cotyledon expiants of eastern black walnut (Juglans nigra L.). Plant Cell Tiss. Org. Cult., 32:9–18.

    Article  Google Scholar 

  • Patri S, Bhatnagar S P and Bhojwani S S (1988) Preliminary investigations on micropropagation of a leguminous timber tree Pterocarpus santalinus. Phytomorphology, 38: 41–45.

    Google Scholar 

  • Phillips G C and Collins G B (1979) In vitro tissue culture of selected legumes and plant regeneration from callus cultures of red clover. Crop Sci., 19: 59–64.

    Article  Google Scholar 

  • Phillips G C and Collins G B (1980) Somatic embryogenesis from cell suspension cultures of red clover. Crop Sci., 20: 323–326.

    Article  CAS  Google Scholar 

  • Phukan M K, and Mitra G C (1982) In vitro regeneration of Albizzia odoratissima Benth, a shade tree for tea plantation of North East India. Two and a Bud, 30: 54–58.

    Google Scholar 

  • Purnhauser L, Medgyesy M, Czeko P J and Marton L (1987) Stimulation of shoot regeneration in Triticum aestivum and Nicotianaplumbaginifolia via tissue culture using ethylene inhibitor AgN03. Plant Cell Rep., 6: 1–4.

    Article  CAS  Google Scholar 

  • Raghavaswamy B V, Kimabindu K and Lakshmi Sita G (1992) In vitro micropropagation of elite rose wood (Dalbergia lati/olia Roxb.). Plant Cell Rep., 11: 126–129.

    Google Scholar 

  • Rahman S M, Hossain M, Biswas B K, Joarder O I and Islam (1993) Micropropagation of Caesalpinia pulcherima through nodal bud culture of mature tree. Plant Cell Tiss. Org. Cult., 32: 363–365.

    Article  CAS  Google Scholar 

  • Ranga Rao G V and Prasad M N V (1991) Plantlet regeneration from the hypocotyl callus of Acacia auriculiformis — multipurpose tree legume. J. Plant Physiol, 173: 625–627.

    Google Scholar 

  • Rao M M and Lakshmi Sita G (1994) Direct somatic embryogenesis from immature embryos of rosewood (Dalbergia latifolia Roxb). Plant Cell Rep., 15: 355–359.

    Google Scholar 

  • Ravishankar Rai V and Chandra K S (1989) In vitro regeneration of plantlets from shoot callus of mature trees of Dalbegia latifolia. Plant Cell Tiss. Org. Cult., 13: 77–83.

    Google Scholar 

  • Rout G R, Samantary S and Das P (1995) Somatic embryogenesis and plant regeneration from callus cultures of Acacia catechu — a multipurpose leguminous tree. Plant Cell Tiss. Org. Cult., 42: 283–285.

    Article  CAS  Google Scholar 

  • Roy S K and Datta S K (1985) Clonal propogation of a legume tree Albizzia procera through tissue culture. Bangladeshi Bot., 14: 127–131.

    Google Scholar 

  • Sankhla D, Davis T D and Sankhla N (1996) In vitro regeneration of silk tree (Albizzia julibrissin) from excised roots. Plant Cell Tiss. Org. Cult., 44: 83–86.

    Article  CAS  Google Scholar 

  • Sankhla D, Sankhla N and Davis T D (1994) Thidiazuron-induced in vitro shoot formation form roots of intact seedlings regeneration of Albizzia julibrissin. Plant Growth Reg., 14: 267–272.

    Article  CAS  Google Scholar 

  • Sankhla D, Sankhla N and Davis T D (1995) Promotion of in vitro shoot formation from excised roots of silk tree (Albizzia julibrissin) by an oxime ether derivative and other ethylene inhibitors. Plant Cell Rep., 15: 115–118.

    Article  Google Scholar 

  • Saxena P K and Gill B (1987) Plant regeneration from mesophyll protoplasts of tree legume — Pithecellobium dulce. Plant Sci., 53: 257–262.

    Article  CAS  Google Scholar 

  • Schenk R U and Hildebrandt A C (1972) Medium and techniques for induction and growth of monocotyledo-nous and dicotyledonous plant cell cultures. Can. J. Bot., 50: 199–204.

    Article  CAS  Google Scholar 

  • Sharma S C and Chandra N C (1988) Organogenesis and plantlet formation in vitro in Dalbergia sissoo Roxb. J. Plant Physiol., 132: 145–147.

    Article  CAS  Google Scholar 

  • Shekhawat N S, Rathore T S, Singh R P, Deora N S and Rao S R (1993) Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Growth Reg., 12: 273–280.

    Article  CAS  Google Scholar 

  • Sinha R K and Mallick R (1993) Regeneration and multiplication of shoots in Albizzia falcataria. Plant Cell Tiss. Org. Cult., 32: 259–261.

    Article  CAS  Google Scholar 

  • Skolmen R G and Mapes M O (1976) Acacia koa — Gray plantlets from somatic callus tissue. J. Hered., 67: 14–115.

    Google Scholar 

  • Thorpe T A, Harry I S and Kumar P P (1991) Application of micropropagation to forestry. In: Micropropagation (Eds Debergh P C and Zimmermann R H), Kluwer Academic Publishers, Dordrecht, The Netherlands, 311–336.

    Chapter  Google Scholar 

  • Tornar U K and Gupta S C (1988a) Somatic embryogenesis and organogenesis in callus cultures of a tree legume -Albizzia richardiana. Plant Cell Rep., 7: 70–73.

    Article  Google Scholar 

  • Tornar U K and Gupta S C (1988b) In vitro plant regeneration of leguminous trees (Albizzia spp.). Plant Cell Rep., 7: 385–388.

    Google Scholar 

  • Upreti J and Dhar U (1996) Micropropagation of Bauhinia vahii Wight and Arnott — a leguminous liana. Plant Cell Rep., 40: 256–264.

    Google Scholar 

  • Van Nieuwkerk J P, Zimmermann R H and Fordham I (1986) Thiadiazuron stimulation of apple shoot proliferation in vitro. Hort. Sci., 21: 516–518.

    Google Scholar 

  • Villarreal M L and Rojas G (1995) In vitro propagation of Mimosa tenuiflora (Willd.) Poiret, a Mexican medicinal tree. Plant Cell Rep., 16: 80–82.

    Article  Google Scholar 

  • Wilde H D, Meagher R B and Merkle S A (1992) Expression of foreign genes in transgenic yellow poplar plants. Plant Physiol, 98: 114–120.

    Article  PubMed  CAS  Google Scholar 

  • Xie D Y and Hong Y (2002) Agrobacterium-mediated genetic transformation of Acacia mangium. Plant Cell Rep., 20: 917–922.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Khurana, P., Khurana, J., Jani, M. (2003). Regeneration and Genetic Transformation of Tree Legumes with Special Reference to Albizzia Species. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_18

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-0139-6_18

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6369-4

  • Online ISBN: 978-94-017-0139-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics