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Agrobacterium-Mediated Transformation of Lotus Species

  • Chapter
Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

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Abstract

Lotus species, and especially Lotus corniculatus, are becoming important alternate forage crops. First, Lotus can be easily grown under a wide range of soil conditions; second, it can be heavily grazed; and third, it does not cause bloat. The development of the culture of Lotus corniculatus is, however, impaired by specificities of that species such as seed shattering, which increase seed cost production, or ploidy number and self-incompatibility, which makes genetic improvement tedious. Genetic engineering of Lotus can help to solve some of the above problems, by allowing the rapid and efficient introduction of agronomically important traits into that species. Several species of Lotus have been transformed using Agrobacterium as a gene transfer system. These include L. corniculatus, L. tenuis, L. angustissimus and L. uliginosus. Lotus species are easily maintained in vivo and in vitro. The efficiency of the regeneration process differs from one species to the other, but overall Lotus species are easily regenerated for calli, explants or cell cultures. In the present chapter, we present a survey of the available protocols for Lotus transformation, with an emphasis on laboratory and procedure tips to allow the people interested in genetic transformation of a novel Lotus species to adapt the existing protocols to the particularities of their favorite species.

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  1. Phil Oger

    Present address: Laboratoire des Sciences de la Terre, ENS-Lyon, 46 allée d’Italie, 69364, Lyon, Cedex of France

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  1. Institut des Sciences Végétales, Bâtiment 23, CNRS, Avenue de la terrasse, F-91198, Gif sur Yvette, France

    Phil Oger & Yves Dessaux

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  1. Phil Oger
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  2. Yves Dessaux
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  1. Department of Biosciences, M.D. University, Rohtak, 124001, India

    Pawan K. Jaiwal  & Rana P. Singh  & 

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Oger, P., Dessaux, Y. (2003). Agrobacterium-Mediated Transformation of Lotus Species. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_17

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  • DOI: https://doi.org/10.1007/978-94-017-0139-6_17

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