Abstract
Since the late 1980s, a bacterium has been routinely isolated from leafspots on geranium (Pelargonium X hortorum L. H. Bailey) and more infrequently from petunia (Petunia hybrida). When inoculated on healthy geranium and petunia leaves, these bacteria caused leafspots identical to those observed on naturally infected geranium plants. Carbon substrate utilisation (BIOLOG) and fatty acid analysis (FAME) identified these isolates to the genus Acidovorax. According to BIOLOG, FAME, and pulsed-field gel electrophoresis (PFGE) data, the geranium and petunia strains formed a very tight phenotypic group that was distinct from other Acidovorax strains tested. In DNA:DNA hybridisation analyses, the geranium strains had greater than 90% homology to each other and to the petunia strain, but less than 50% homology to other Acidovorax species with the exception of A. konjaci (52%). Sequence analysis of the 16S rRNA revealed that the geranium and petunia strains had 99% homology with A. konjaci. Based on PFGE, BIOLOG, FAME, and DNA:DNA hybridisation results, the geranium petunia strains are very closely related to each other and are most closely related to A. konjaci.
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References
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., and Seidman, K., 1987, Current protocols in molecular biology. John Wiley and Sons, New York.
Egel, D., Graham, J., and Stall, R.E., 1991, Genomic relatedness of Xanthomonas campestris strains causing diseases of citrus. Appl. Env. Microbiol. 57: 2724–2730.
Ezaki, T., Hashimoto, Y., and Yabuuchi, E., 1989, Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridisation in microdilution wells as an alternative to membrane filter hybridisation in which radioisotopes are used to determine genetic relatedness among bacterial strains. Inst. J. Syst. Bacteriol. 39: 224–229.
Felsenstein, J., 1993, PHYLIP (Phylogeny Inference Package). Version 3.57. Computer program and manual distributed by the author.
Gardan, L, Dauga, C., Prior, P., Gillis, M., and Saddler, G.S., 2000, Acidovorax anthurii sp. nov., a new phytopathogenic bacterium which causes bacterial leaf-spot of anthurium. Int. J. Syst. Evol. Microbiol. 50: 235–246.
Innes, M.A., Gelfand D.H., and Sninsky, J.J., 1995. PCR Strategies. Academic Press, New York, NY.
Johnson, J.L., 1994, Similarity analysis of DNAs. Pp. 655–682. In: Methods for general and molecular bacteriology. Gerhardt, P., Murray, T.G.E., and Krieg, N.R (ed.). American Society for Microbiology, Washington, DC.
Jones, J.B., Bouzar, H., Stall, R.E., Almira, E.C., Roberts, P., Bowen, B.W., Sudberry, J., Strickler, P. Chun, J., 2000, Systematic analysis of xanthomonads (Xanthomonas spp.)
asscoiated with pepper and tomato lesions. Int. J. Syst. Bacteriol. 50: 1211–1219.
Jones, J.B., Stall, R.E., Somodi, G.C., Bouzar, H., and Hodge, N.C., 1995, A third tomato race of Xanthomonas campestris pv. vesicatoria. Plant Dis. 79: 395–398.
Ponsonnet, C., and Nesme, X., 1994, Identification of Agrobacterium strains by PCR-RFLP analysis of pTi and chromosomal regions. Arch. Microbiol. 161: 300–309.
Simone, G.W., Cullen, R.E., and Hodge, N.C., 1996, A new leaf spot disease of geranium caused by Acidovorax sp. Phytopathology 86: S50.
Willems, A., Falsen, E., Pot, B., Jantzen, E., Hoste, B., Vandamme, P., Gillis, M., Kersters, K., and De Ley, J., 1990, Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovoraxfacilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. Nov. Int. J. Syst. Bacteriol. 40: 384–398.
Willems, A., Goor, M., Thielemans, S., Gillis, M., Kersters, K., and De Ley J., 1992, Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. Int. J. Syst. Bacteriol. 42: 107–119.
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Jones, J.B. et al. (2003). Characterisation of an Acidovorax sp. Associated with Geranium and Petunia. In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_73
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DOI: https://doi.org/10.1007/978-94-017-0133-4_73
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