Abstract
Bacterial canker and decline, caused by Pseudomonas avellanae, is seriously damaging the hazelnut cultivation in central Italy and in northern Greece. Surveys carried out in the other main areas of cultivation in Italy ascertained the presence of strains similar to P. syringae pv. syringae van Hall. In these areas severe epidemics of bacterial decline were not observed. The taxonomic relationships among the pseudomonads associated with hazelnut decline and the other plant pathogenic Pseudomonas spp. has been established by using a collection of more than 200 strains by means of ARDRA, repetitive PCR fingerprints using ERIC, REP and BOX primer sets with UPGMA analysis, 16S rRNA gene sequencing as well as by analysis of fatty acid methyl ester and whole-cell protein profiles, and by pathogenicity tests. P. avellanae belongs to genomospecies 8, sensu Gardan et al., which also includes P. s. pv. theae and P. s. pv. actinidiae. P. avellanae shows a pathogenicity restricted to Corylus avellana and is very virulent to hazelnut germplasm. The P. s. pv. syringae-like strains isolated from hazelnut fit well with the main features of the pathovar, including the presence of the syrB gene. A population found in Piedmont appears different from the P. s. pv. syringae-like strains when analysed by fatty acid profiling. In addition, the strains do not have the syrB gene and show a very restricted pathogenicity. This population might be a new pathovar within the P. syringae complex.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fiori, M., Cicconi, L., and Scortichini, M., 2003, Bacterial Canker of Hazelnut (Corylus avellana L.) in Sardinia: Occurrence of Pseudomonas syringae Strains, 617–625, (this volume).
Gardan, L., Shafik, H., Belouin, S., Brosch, R., Grimont, F., and Grimont, P.A.D., 1999, DNA relatedenees among the pathovars of Pseudomonas syringae and description of Pseudomonas tremae sp. nov. and Pseudomonas cannabina sp. nov. (ex Sutic and Dowson). Int. J. Syst. Bacteriol. 49: 469–478.
Jackson, R.W., Mansfield, J W, Ammouneh, H., Dutton, L.C., Wharton, B., Ortiz-Barredo, A., Arnold, D.L., Tsiamos, G., Sesma, A., Butcher, D., Boch, J., Kim, Y-J, Martin, G.B., Tegli, S., Murillo, J., and Vivian, A., 2002, Location and activity of member of a family of virPphA homologues in pathovars of Pseudomonas syringae and P. savastanoi. Mol. Pl. Pathol. 3 (4): 205–216.
Janse, J.D., Rossi, M.P., Angelucci, L., Scortichini, M., Derks, J.H.J., Akkermans, A.D.L., De Vrijer, R., and Psallidas, P.G., 1996, Reclassification of Pseudomonas syringae pv. avellanae as P. avellanae (spec. nov), the bacterium causing canker of hazelnut (Corylus avellana L.). Syst. Appl. Microbiol. 19: 589–595.
Lelliott, R.A., Billing, E., and Hayward, A.C., 1966, A determinative scheme for the fluorescent plant pathogenic Pseudomonas. J. Appl. Bacteriol. 29: 470–489.
Loreti, S., Sarrocco, S., and Gallelli, A., 2001, Identification of hrp gene, encoding harpin, in Pseudomonas avellanae (Psallidas) Janse et al. J. Phytopath. 149: 219–226.
Loreti, S., and Gallelli, A., 2002, Rapid and specific detection of virulent Pseudomonas avellanae strains by PCR amplification. Eur. J Pl. Pathol. 108: 237–244.
Ovod, V.V., Knirel, Y.A., Samson, R., and Krohn, K.J., 1999, Immunochemical characterization and taxonomic evaluation of the O polysaccharide of the lipopolysaccharides of Pseudomonas syringae serogroup 01 strains. J. Bacteriol. 181: 6937–6947.
Psallidas, P.G., and Panagopolous C.G., 1979, A bacterial canker of hazelnut in Greece caused by Pseudomonas syringae pv. avellanae. Phytopath. Z. 94: 103–111
Scortichini, M., and Tropiano, F.G., 1994, Severe outbreak of Pseudomonas syringae pv. avellanae on hazelnut in Italy. J. Phytopath. 140: 65–70.
Scortichini, M., and Lazzari, M., 1996, Systemic migration of Pseudomonas syringae pv. avellanae in twigs and young trees of hazelnut and symptom development. J Phytopath. 144: 215–219.
Scortichini. M., and Marchesi, U., 2001, Sensitive and specific detection of Pseudomonas avellanae strains using primers based on 16S rRNA gene sequences. J. Phytopath. 149: 527–532.
Scortichini, M., Marchesi, U., and Di Prospero, P., 2002a, Genetic relatedeness among Pseudomonas avellanae, P. syringae pv. theae and P. s. pv. actinidiae, and their identification. Eur. J. Pl. Pathol. 108: 269–278.
Scortichini, M., Marchesi, U., Rossi, M.P. and Di Prospero, P., 2002b, Bacteria associated with hazelnut (Corylus avellana L.) decline are of two groups: Pseudomonas avellanae and strains resembling P. syringae pv. syringae Appl. Env. Microbiol. 68: 476–484.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Scortichini, M., Marchesi, U., Rossi, M.P., Janse, J.D., Stead, D.E. (2003). The Pseudomonads Associated with Bacterial Canker and Decline of Hazelnut (Corylus avellana L.). In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_64
Download citation
DOI: https://doi.org/10.1007/978-94-017-0133-4_64
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6267-3
Online ISBN: 978-94-017-0133-4
eBook Packages: Springer Book Archive