Abstract
Pseudomonas syringae strain 1.1S and P. fluorescens strain A506 were applied to tomato plants to investigate their ability to survive during plant growth and ripening of fruits. Both strains were used to spray tomato flowers and green tomato fruits. Ripe tomato fruits were harvested and subjected to microbiological analysis. Peptone wash water and homogenates of fruit pulp were plated on selective media before and after enrichment. All tomatoes produced from inoculated flowers contained P. syringae on the surface of fruits (100% in enriched samples) as well as in the pulp homogenates (100% before and after enrichment). All tomatoes produced from inoculated green fruits contained P. syringae on the surface (38% and 100% in wash water and in enriched samples, respectively) as well as in the pulp homogenates (90% and 100% before and after enrichment, respectively). Of the P. fluorescens surface-positive tomatoes, 37% were from fruits receiving flower inoculation and 77% were from fruits receiving green fruit inoculation. P. fluorescens was harboured in higher percentages in pulp homogenates of tomatoes produced from treated flowers and green fruits (62% and 100%, respectively). Results suggest that antagonistic P. syringae and P. fluorescens strains survive in and on tomato fruits from the time of inoculation at flowering or at early stage of fruit development through fruit ripening. Tomato flower and green surfaces of fruits are possible sites at which Pseudomonas may attach and remain viable during fruit development.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bartz, J.A., 1982, Infiltartion of tomatoes immersed at different temperatures to different depths in suspensions of Erwinia carotovora subsp. carotovora. Plant Dis. 66: 302–306.
Buchanan, R.L., Edelson, S.G., Miller, R.L., and Sapers, G.M., 1999, Contamination of intact apples after immersion in an aqueosus environment containing Escherichia coli 0157:H7 J. Food Protec. 62: 444–450.
Cirvilleri, G., Bella, P., and Catara V., 2000, Molecular detection and biological control activity of Pseudomonas strains. 5Congress of the European Foundation for Plant Pathology, Taormina, 18–22 September 2000, 534–538.
Cirvilleri, G., and Caldarera, G., 1998, Use of lux-marker genes to monitor survival of antagonistic Pseudomonas fluorescens in the phylloplane. J. Pl. Dis. Protec. 105: 441–451.
Ercolani, G., and Casolari, A., 1966, Ricerche di microflora in pomodori sani. Industrie Conserve Parma 41: 15–22.
Nguyen-the, C., and Carlin, F.C., 1994, The microbiology of minimally processed fresh fruits and vegetables. Crit. Rev.Food Sci. Nu. 34: 371–401.
O’Sullivan, D.J., and O’Gara, F., 1992, Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiol. Rev. 56: 662–676.
Samish, Z., Etinger-Tulczynska, R., and Bick, M., 1962, The microflora within the tissue of fruits and vegetables. J. Food Sci. 28: 259–266.
Schena, L., Sialer, M.F., and Gallitelli, D., 2002, Molecular detection of strain L47 of Aureobasidium pullulans, a biocontrol agent of postharvest diseases. Pl. Dis. 86: 54–60.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Pacetto, A., Bella, P., Catara, V., La Rosa, R., Cirvilleri, G. (2003). Survival of Two Biocontrol Pseudomonas Strains in Tomato Fruits After Inoculation at Flowering Through Fruit Ripening. In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_6
Download citation
DOI: https://doi.org/10.1007/978-94-017-0133-4_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6267-3
Online ISBN: 978-94-017-0133-4
eBook Packages: Springer Book Archive