Abstract
Avirulent pathogenic bacteria often cause a hypersensitive response (HR) in plants, an effective resistance phenomenon coupled to programmed cell death (PCD) and usually an increased production of reactive oxygen species (ROS) in the infected cells. HR induction is dependent on Ca2+ accumulation/influx within the plant cytoplasm. Here we show that Lanthanum (La3+), a Ca2+ channel blocker, inhibited PCD and H202 accumulation in tobacco leaf tissue injected with Pseudomonas savastanoi pv. phaseolicola, an avirulent pathogen, even if it was added up to 4.5 h after the bacterium. This was 2 h after the HR induction phase and the beginning of an intense ROS accumulation in the control (HR-positive) leaf tissue. However, La3+ did not inhibit transcription of the pathogenesis-related protein PR-la gene and only partially blocked the HR-associated resistance. We conclude that during HR: 1) Ca2+ influx is needed not only to trigger but also to maintain ROS production and processes leading to PCD; 2) There is a PCD- and ROSindependent resistance mechanism. The comparison of this “remaining” resistance with the early induced resistance (EIR) needs further analysis.
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Ott, P.G., Klement, Z., Nagy, I., Ádám, A.L. (2003). Lanthanum Inhibits Programmed Cell Death but not Resistance in the Tobacco — Pseudomonas savastanoi pv. phaseolicola Incompatible Interaction. In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_36
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DOI: https://doi.org/10.1007/978-94-017-0133-4_36
Publisher Name: Springer, Dordrecht
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