Abstract
Plant pathogenic bacteria carry a large set of virulence genes that enables them to exploit plant hosts to support their growth. A genetic screen (IVET) was established to identify bacterial genes that are expressed during the infection process. About one third of the in planta expressed (ipx) genes were shown to be coregulated with the Hrp-type III secretion system. The hrp-coregulated ipx genes were compared to putative virulence genes that were identified by other genomic screens. A subset of the hrp-coregulated ipx genes encode known effectors that are translocated by the Hrp-system into the plant cell as well as putative effectors. Using a reporter system for translocation we were able to identify two novel effectors. Further studies will be aimed at characterizing the effect of these virulence factors in the plant cell.
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Boch, J., Landgraf, A., Krause, J., Kunkel, B.N., Bonas, U. (2003). Identification of Virulence Factors from Pseudomonas syringae . In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_24
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DOI: https://doi.org/10.1007/978-94-017-0133-4_24
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6267-3
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