Abstract
In this study, we reported the production of an antimetabolite toxin (named mangotoxin), mainly by strains of Pseudomonas syringae pv. syringae pathogenic to mango. The target of mangotoxin is the enzymatic step where ornithine is produced from N-acetyl ornithine in the biosynthetic pathway of arginine. The inhibition of ornithine acetyltransferase (OAT) activity by mangotoxin was confirmed by exposing tomato leaf protein extracts to filtrates from cultures of P. s. pv. syringae. Mutants impaired in the production of mangotoxin were constructed. Sequencing of Tn5 flanking regions of 9 non/low-producers mutants revealed that several genes codifying for putative acetyltransferase, non-ribosomal peptide synthetase and the global regulators proteins GacA and LemA could be involved in mangotoxin production. Mangotoxin also showed antimicrobial activity against some micro-organisms, usually found on plant tissues as epiphytic or pathogenic micro-organisms. To conclude, the possible role of the mangotoxin in pathogenesis is discussed.
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References
Alstchul, S.E., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J., 1997, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.
Bender, C.L., Alarcón-Chaidez, F., and Gross, D.C., 1999, Pseudomonas syringae phytotoxins: Mode of action, regulation, and biosynthesis by peptide and polyketide synthetases. Microbiol. Mol. Biol. Rev. 63: 266–292.
Cazorla, F.M., Olalla, L., Torés, J.A., Codina, J.C., Pérez-Garcia, A., and de Vicente, A., 1997, Pseudomonas syringae pv. syringae as microorganism involved in apical necrosis of mango: characterization of some virulence factors. In “Pseudomonas syringae Pathovars and Related Species”. Ed. K. Rudolph, J.J. Burr, J.W. Mansfield, D. Stead, A. Vivian, and J. Von Kietzell. Kluwer Academic Publishers. Dordrecht. The Netherlands. pp. 82–87.
Cazorla, F.M., Torés, J.A., Olalla, L., Pérez-Garcia, A., Farré, J.M., and de Vicente, A., 1998, Bacterial apical necrosis of mango in Southern Spain: a disease caused by Pseudomonas syringae pv. syringae. Phytopathology 88: 614–620.
De Lorenzo, V., and Timmis, K.N., 1994, Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5 and Tn10-derived mini-transposons. Methods Enzymol. 235: 386–406.
Denés, G., 1970, Ornithine acetyltransferase (Chlamydomonas reinhardti). Methods Enzymol. 17A: 273–277.
Durbin, R.D., 1991, Bacterial phytotoxins: mechanisms of action. Experientia 47: 776–783.
Gasson, M.J., 1980, Indicator technique for antimetabolite toxins production by phytopathogenic species of Pseudomonas. App/. Environ. Microbiol. 39: 25–29.
Gross, D.C., and De Vay, J.E., 1977, Production and purification of syringomycin, a phytotoxin produced by Pseudomonas syringae. Physiol. Plant Pathol. 11: 13–28.
Iacobellis, N.S., and Latorraca, M. 1999, Production of antimetabolites by Pseudomonas syringae pathovars. Molecular Plant-Microbe Interactions, 9th International Congress. Amsterdam (The Netherlands). pp. 180.
Iacobellis, N.S., Lavermicocca, P., Grgurina, I., Simmaco, M., and Ballio, A., 1992, Phytotoxic properties of Pseudomonas syringae pv. syringae toxins. Physiol. Mol. Plant Pathol. 40: 107–116.
Pérez-Garcia, A., Cénovas, F.M., Gallardo, F., Hirel, B., and de Vicente, A., 1995, Differential expression of glutamine synthetase isoforms in tomato detached leaflets infected with Pseudomonas syringae pv. tomato. Mol. Plant-Microbe Interact. 8: 96–103.
Sinden, S.L., de Vay, J.E., and Backman, P A, 1971, Properties of syringomycin, a wide spectrum antibiotic and phytotoxin produced by Pseudomonas syringae, and its role in the bacterial canker disease of peach trees. Physiol. Plant Pathol. 1: 199–213.
Volksch, B., and Weingart, H., 1998, Toxin production by pathovars of Pseudomonas syringae and their antagonistic activities against epiphytic microorganisms. J. Basic Microbiol. 38: 135–145.
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Cazorla, F.M. et al. (2003). An Antimetabolite Toxin (Mangotoxin) is Produced by Pseudomonas syringae pv. syringae Isolated from Mango. In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_19
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DOI: https://doi.org/10.1007/978-94-017-0133-4_19
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