Abstract
Pseudomonas syringae strains express a hrp-encoded type III protein secretion system (TTSS) during pathogenesis that injects effector proteins into the cytoplasm of host cells. A complex regulatory system controlling hrp regulon expression has recently been characterised that involves positive and negative regulatory factors. The interaction of these regulatory factors and their roles are discussed. Several genetic screens for effectors that employ components of the regulatory system are presented.
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Alfano, J.R., Charkowski, A.O., Deng, W.-L., Badel, J.L., Petnicki-Ocwieja, T., van Dijk, K., and Collmer, A., 2000, The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic structure composed of a cluster of type III secretion genes bound by exchangeable effector and conserved effector loci that contribute to parasitic fitness and pathogenicity in plants. Proc. Natl. Acad. Sci. USA 97: 4856–4861.
Bretz, J., Lisboa, K., Thareja, N., Losada, L., and Hutcheson, S.W., 2002, Lon protease functions as a negative regulator of type III protein secretion in Pseudomonas syringae. Mol. Microbiol. 45: 397–409.
Charity, J., Pak, K.C., Delwiche, and Hutcheson, S., 2003, Novel exchangeable effector loci associated with the hrp pathogenicity island of P. syringae: evidence for integron-like assembly from transposed gene cassettes. Mol. Plant-Microbe Interact. 16: (in press).
Chen, Z., Kloek, A.P., Boch, J., Katagiri, F., and Kunkel, B.N., 2000, The Pseudomonas syringae avrRpt2 gene product promotes pathogen virulence from inside plant cells. Mol. Plant-Microbe Interact. 13: 1312–1321.
Dangl, J., and Jones, J., 2001, Plant pathogens and integrated defence responses to infection. Nature (London) 411: 826–833.
Fouts, D., Abramovitch, R., Alfano, J., Baldo, A., Buell, C., Cartinhour, S., Chatterjee, A., D’Ascenzo, M., Gwinn, M., Lazarowitz, S., Lin, N.-C., Martin, G., Rehm, A., Schneider, D., vanDijk, K., Tang, X., and Collmer, A., 2002, Genomewide identification of Pseudomonas syringae pv tomato DC3000 promoters controlled by the HrpL alternative sigma factor. Proc. Natl. Acad. Sci. USA 99: 2275–2280.
Galan, J.E., and Collmer, A., 1999, Type III secretion machines: bacterial devices for protein delivery into host cells. Science 284: 1322–1328.
Gottesman, S., 1996, Proteases and their targets in Escherichia coli. Annu. Rev. Genet. 30: 465–506.
Greenberg, J.,T., Vinatzer B.N., and Jelenska J., 2002, Genetic and genomic analysis of Pseudomonas syringae effectors. Book of Abstracts, 6th International Conference on Pseudomonas syringae Pathovars and Related Pathogens, September 15 — 19, 2002, Acquafredda di Maratea (PZ), Italy, p. 23.
Grimm, C, Aufsatz, W., and Panopoulos, N.J., 1995, The hrpRS locus of Pseudomonas syringae pv. phaseolicola constitutes a complex regulatory unit. Mol. Microbiol. 15: 155–165.
Hutcheson, S.W., 1999, The hrp cluster of Pseudomonas syringae: a pathogenicity island encoding a type III protein translocation complex?, p. 309–329. In: J. B. Kaper and J. Harker (ed.), Pathogenicity islands and other mobile virulence elements. Amer. Soc. Microbiol. Washington, D.C.
Hutcheson, S.W., 2001, The molecular biology of hypersensitivity to plant pathogenic bacteria. I Plant Pathol. 83: 151–172.
Hutcheson, S.W., Bretz, J., Sussan, T., Jin, S., and Pak, K., 2001, The enhancer binding protein HrpR and HrpS interact to regulate hrp-encoded type III protein secretion in Pseudomonas syringae strains. J. Bacteriol. 183: 5589–5598.
Jackson, R., Mansfield, J., Arnold, D., Sesma, A., Paynter, C., Murillo, J., Taylor, J., and Vivian, A., 2000, Excision from tRNA genes of a large chromosomal region, carrying avrPphB, associated with race change in the bean pathogen, Pseudomonas syringae pv. phaseolicola. Mol. Microbiol. 38: 186–197.
Jin, Q., and He, S., 2002, Role of the Hrp pilus in type III protein secretion in Pseudomonas syringae. Science 294: 2556–2558.
Kim, J.F., Charkowski, A.O., Alfano, J.R., Collmer, A., and Beer, S.V., 1998, Sequences related to transposable elements and bacteriophages flank avirulence genes of Pseudomonas syringae. Mol. Plant-Microbe Interact. 11: 1247–1252.
Kuroda, A., Nomura, K., Ohtomo, R., Kato, J., Ideda, T., Takiguchi, N., Ohtake, H., and Kornberg, A., 2001, Role of inorganic polyphosphate in promoting ribosomal protein degradation by the Lon protease in E. coli. Science 293: 705–708.
Lee, J., Klusener, B., Tsiamis, G., Stevens, C., Neyt, C., Tampakaki, A.P., Panopoulos, N.J., Noller, J., Weiler, E., Cornelis, G.R., Mansfield, J.W. and Nurnberger, T., 2001, HrpZp h from the plant pathogen Pseudomonas syringae pv. phaseolicola binds to lipid bilayers and forms an ion-conducting pore in vitro. Proc. Natl. Acad. Sci. USA 98: 289–294.
Lee, J.L., Scholl, D., Nixon, B.T., and Hoover, T., 1994, Constitutive ATP hydrolysis and transcription activation by a truncated form of Rhizobium meliloti DctD, a 654-dependent transcriptional activator. J. Biol. Chem. 269: 20401–20409.
Osuna, J., Soberon, X., and Morett, E., 1997, A proposed architecture for the central domain of the bacterial enhancer-binding proteins based on secondary structure prediction and fold recognition. Prot. Sci. 6: 543–555.
Pirhonen, M.U., Lidell, M.C., Rowley, D., Lee, S.W., Silverstone, S., Liang, Y., Keen, N.T. and Hutcheson, S.W., 1996, Phenotypic expression of Pseudomonas syringae avr genes in E. coli is linked to the activities of the hrp-encoded secretion system. Mol. Plant-Microbe Interact. 9: 252–260.
Preston, G., Deng, W.-L., Huang, H.-C. and Collmer, A., 1998, Negative regulation of hrp genes in Pseudomonas syringae by HrpV. J. Bacteriol. 180: 4532–4537.
Rahme, L.G., Mindronos, M.N., and Panopoulos, N.J., 1992, Plant and environmental sensory signals control the expression of hrp genes in Pseudomonas syringae pv. phaseolicola. J. Bacteriol. 174: 3499–3507.
Xiao, Y., Lu, Y., Heu, S., and Hutcheson, S.W., 1992, Organisation and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster. J. Bacteriol. 174: 1734–1741.
Xiao, Y., Heu, S., Yi, J., Lu, Y. and Hutcheson, S.W., 1994, Identification of a putative alternate sigma factor and characterisation of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes. J. Bacteriol. 176: 1025–1036.
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Hutcheson, S.W., Bretz, J.R., Charity, J.C., Losada, L., Sussan, T. (2003). Regulation and Detection of Effectors Translocated by Pseudomonas syringae . In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_16
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DOI: https://doi.org/10.1007/978-94-017-0133-4_16
Publisher Name: Springer, Dordrecht
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