Abstract
The molecular analysis of pathogenicity and virulence in Pseudomonas syringae is hampered by functional gene redundancy and by the usually small contribution of each individual virulence gene. In consequence, classical techniques, such as transposon mutagenesis, have proved of limited use for the identification of virulence genes. Curing of native plasmids, however, has allowed the identification of diverse virulence genes and pathogenicity islands in several P. syringae pathovars. In this paper, we summarise the strategies and techniques that we have used successfully for the eviction of native plasmids and the analysis of the resulting strains.
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Murillo, J., Butcher, D., Jackson, R., Sundin, G.W., Vivian, A., Arnold, D. (2003). Methods for the Identification of Virulence Genes in Pseudomonas syringae . In: Iacobellis, N.S., et al. Pseudomonas syringae and related pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0133-4_14
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DOI: https://doi.org/10.1007/978-94-017-0133-4_14
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6267-3
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