Abstract
Legumes are one of the most significant groups of economically important crops and have therefore been the subject of efforts to improve desirable traits through in vitro manipulations. Efforts are directed for developing efficient regeneration system in order to facilitate development of a variety of technologies. Even though many legumes have undergone de novo regeneration, limited contributions to crop improvement have been achieved. Successful regeneration is, in many cases, genotype and explant specific. In recent years, focus has been on development of plant regeneration systems amenable for gene transfer technology. Organogenesis has been utilized in many cases for transformation, but somatic embryogenesis shows a great promise as prolific production system for gene transfer for those legumes which do not regenerate via organogenesis. Currently, sufficient knowledge is available only for designing and optimizing de novo regeneration systems for many legumes, but not for successful and hassle-free genetic transformation.
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Mohan, M.L., Krishnamurthy, K.V. (2003). In Vitro Morphogenesis in Grain Legumes: An Overview. In: Jaiwal, P.K., Singh, R.P. (eds) Improvement Strategies of Leguminosae Biotechnology. Focus on Biotechnology, vol 10A. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0109-9_2
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