Abstract
Liposomes are micro-compartments made of lipid bilayer membranes with characteristics quite similar to those of biological membranes. To form artificial cell-like structures, we generated liposomes that contained subunit proteins of cytoskeletons: tubulin or actin. Spherical liposomes were transformedinto bipolar or cell-like shapes by mechanical forces generated by the polymerization of encapsulated subunits of microtubules. Disk- or dumbbell-shaped liposomes were developed by the polymerization of encapsulated actin. Dynamic processes of morphological transformations of liposomes were visualized by high intensity dark-field light microscopy.
Topological changes, such as fusion and division of membrane vesicles, play an essential role in cellular activities. To investigate the mechanism of these processes, we visualized in real time the liposomes undergoing topological transformation. A variety of novel topological transformations were found, including the opening-up of liposomes and the direct expulsion of inner vesicles.
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Hotani, H. et al. (2002). Morphological and Topological Transformation of Liposomes. In: Drew, H.R., Pellegrino, S. (eds) New Approaches to Structural Mechanics, Shells and Biological Structures. Solid Mechanics and Its Applications, vol 104. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9930-6_33
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DOI: https://doi.org/10.1007/978-94-015-9930-6_33
Publisher Name: Springer, Dordrecht
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