Abstract
We have developed a gene expression system for use in the lactic acid bacterium Lactococcus lactis. The expression is controlled by the L. lactis promoter, P170, which is up-regulated by low-pH during the transition to stationary phase. The P170 promoter is coupled to a modified L. lactis signal peptide, SP310mut2, to mediate secretion of the recombinant protein. Optimisation of genetic components, growth conditions and medium composition has resulted in the production of approximately 300 mg of secreted protein per litre culture. We have successfully produced a number of heterologous proteins from both prokaryotic and eukaryotic organisms. The system is currently used for the production of bacterial vaccine components for use in humans.
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© 2001 Springer Science+Business Media Dordrecht
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Bredmose, L. et al. (2001). Development of a Heterologous Gene Expression System for Use in Lactococcus Lactis . In: Merten, OW., et al. Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9749-4_19
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DOI: https://doi.org/10.1007/978-94-015-9749-4_19
Publisher Name: Springer, Dordrecht
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