Abstract
A fed-batch process is described where the effect of the growth rate at the point of induction is discussed. The model process is based on ß-galactosidase production induced by the lacUV5 promoter on a low copy number plasmid. The total amount of accumulated product was approximately 25 % of the total protein. Induction was performed at high and low growth rate. The resulting product formation was very different and twice as high production rate was achieved at high pre-induction growth rate compared to the low rate. It was seen that this was mainly due to translational limitation since the mRNA reached the same level at both occasions while the amount of ribosomes was at low preinduction growth rate only 1/5 of the value at the high growth rate induction.
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© 2001 Springer Science+Business Media Dordrecht
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Sanden, A.M., Larsson, G. (2001). Control and Optimisation of Cellular Bottlenecks in Recombinant Protein Production. In: Merten, OW., et al. Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9749-4_16
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DOI: https://doi.org/10.1007/978-94-015-9749-4_16
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5756-3
Online ISBN: 978-94-015-9749-4
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