Skip to main content
SpringerLink
Log in
Book cover

Advances in Prostaglandin and Leukotriene Research pp 17–20Cite as

Calcium Binding Site of 5-Lipoxygenase

  • Chapter

  • 85 Accesses

Part of the book series: Medical Science Symposia Series ((MSSS,volume 16))

Abstract

5-lipoxygenase (5LO) catalyzes the formation of leukotriene A4 from arachidonic acid. Factors determining 5LO enzyme activity are calcium, phosphatidylcholine (membranes), ATP, the cellular redox status, and phosphorylation events. Ionophores (increase intracellular Ca2+) are effective, and Ca2+ also activates 5LO in vitro. 5LO binds Ca2+ in a reversible manner with a Kd close to 6 µM and maximum binding around two Ca2+ per 5LO [1]. While examining the Ca2+ binding site on 5LO, we noticed similarity between the 5LO N-terminal domain, and calcium binding C2-domains of for example cPLA2. Here, we describe that the N-terminal domain of 5LO binds calcium and mediates calcium stimulation of enzyme activity.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Hammarberg T, Rådmark O. 5-lipoxygenase binds calcium. Biochemistry 1999; 38: 4441–47.

    Article  PubMed  CAS  Google Scholar 

  2. Gillmor SA, Villasenor A, Fletterick R, Sigal E, Browner MF. The structure of mammalian 15-lipoxygenase reveals similarity to the lipases and the determinants of substrate specificity. Nat Struct Biol 1997; 4: 1003–9.

    Article  PubMed  CAS  Google Scholar 

  3. Perisic O, Fong S, Lynch DE, Bycroft M, Williams RL. Crystal structure of a calcium- phospholipid binding domain from cytosolic phospholipase A2. J Biol Chem 1998; 273: 1596–1604.

    Article  PubMed  CAS  Google Scholar 

  4. Nalefski EA, Falke JJ. The C2 domain calcium-binding motif: structural and functional diversity. Protein Sci 1996; 5: 2375–90.

    Article  PubMed  CAS  Google Scholar 

  5. Reddy KV, Hammarberg T, Rådmark O. Mg activates 5-lipoxygenase in vitro: dependency on concentrations ofphosphatidylcholine and arachidonic acid. Biochemistry 2000; 39: 1840–48.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. Tove Hammarberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Patrick Provost
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Konda Veera Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bengt Persson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Olof Rådmark
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, S-171 77, Stockholm, Sweden

    Bengt Samuelsson

  2. Department of Pharmacological Sciences, University of Milan, Milan, Italy

    R. Paoletti , Giancarlo C. Folco  & S. Nicosia ,  & 

  3. Center for Cardiopulmonary Pharmacology, Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133, Milan, Italy

    Giancarlo C. Folco

  4. Karolinska Hospital, Stockholm, Sweden

    E. Granström

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Hammarberg, T., Provost, P., Reddy, K.V., Persson, B., Rådmark, O. (2001). Calcium Binding Site of 5-Lipoxygenase. In: Samuelsson, B., Paoletti, R., Folco, G.C., Granström, E., Nicosia, S. (eds) Advances in Prostaglandin and Leukotriene Research. Medical Science Symposia Series, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9721-0_3

Download citation

  • DOI: https://doi.org/10.1007/978-94-015-9721-0_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5881-2

  • Online ISBN: 978-94-015-9721-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation