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Transgenic Pest and Disease Resistant White Clover Plants

  • Conference paper
Molecular Breeding of Forage Crops

Part of the book series: Developments in Plant Breeding ((DIPB,volume 10))

Abstract

Both insect pests and viral diseases have significant negative impacts on the yield and persistence of forage legumes, and often it is difficult to develop resistant germplasm using traditional plant breeding approaches. We illustrate some of the principles for the molecular breeding of forage legumes, by demonstrating how cloned resistance genes can be used to produce transgenic white clover plants, resistant to either an insect pest (Wiseana spp.) or white clover mosaic virus (WC1MV). Insect bioassays identified a Bacillus thuringiensis Cry1Ba toxin and the proteinase inhibitor, BPTI, as possible resistance factors for Wiseana larvae. Transgenic white clover plants expressing a modified crylBa gene, altered to give an increased proportion of G/C bases and mimic the codon use of plant genes, were toxic to feeding Wiseana larvae. While larvae feeding on white clover expressing the BPTI transgene survived, their growth rate was significantly reduced. Our strategy to develop resistance to WC1MV has been to express individual WC1MV genes in transgenic white clover plants. Expression of the coat protein gene of WC1MV in white clover gave low levels of resistance to the virus, but a substantial delay to systemic infection. Expression of a mutated form of the WC1MV 13K movement protein gene in white clover, resulted in plants with a high level of resistance and restricted systemic spread of the virus. Five of 22 transgenic white clover plants designed to express the WC1MV replicase gene showed immunity to WC1MV infection.

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References

  • Allen PG (1989) Arthropod pests and the persistence of pasture pests in Australia. In: Marten GC, Matches AG, Barnes RF, Brougham RW, Clements RJ, Sheath GW (eds.) Persistence of forage legumes. American Soc Agron, CSSA, SSSA, Madison, Wisconsin, USA, pp 419–439.

    Google Scholar 

  • Barnett OW, Gibson PB (1977) Effect of virus infection on flowering and seed production of the parental clones of tillman white clover (Trifolium repens). Plant Dis Repr 61: 203–207.

    Google Scholar 

  • Beck DL, Forster RSL, Bevan MW, Boxen KA, Lowe SC (1990) Infectious transcripts and nucleotide sequence of cloned cDNA of the potexvirus white clover mosaic virus. Virology 177: 152–158.

    Article  PubMed  CAS  Google Scholar 

  • Beck DL, Van Dolleweerd CJ, Dudas B, White DWR, Forster RLS (1993) Coat protein-mediated protection against white clover mosaic virus and potato virus X in tobacco. Proc XVII Int Grass Conf. pp 1173–1175.

    Google Scholar 

  • Beck DL, Van Dolleweerd CJ, Lough TJ, Balmori E, Voot DM, Anderson MT, O’Brien IEW, Forster RLS (1994) Disruption of virus movement confers broad spectrum resistance against systemic infection by plant viruses with a triple gene block. Proc Natl Acad Sci (USA) 91: 10310–10314.

    Article  CAS  Google Scholar 

  • Beck DL, Eagles RM, Balmori E, Voot D, Lough TJ, Forster RLS (1996) gene silencing associated with resistance to virus infection. In: Grierson D, Lycett GW, Tucker GA (eds.) Mechanisms and applications of gene silencing. Nottingham Univ Easter Seminar Series, Nottingham Univ Press, Nottingham, pp 149–157.

    Google Scholar 

  • De Rocher EJ, Vargo-Gogola TC, Diehn SH, Green PJ (1998) Direct evidence for rapid degradation of Bacillus thuringiensis toxin mRNA as a cause of poor expression in plants. Plant Physiol 117: 1445–1461.

    Article  PubMed  Google Scholar 

  • Burgess EPJ, Gatehouse AMR (1997) engineering for insect pest resistance. In: McKersie BD, Brown DCW (eds.) Biotechnology and the improvement of forage legumes. CAB International, pp 229–258.

    Google Scholar 

  • Burgess EPJ, Main CA, Stevens PS, Gatehouse AMR, Christeller JT, Laing WA (1993) Protease inhibitors active against porina caterpillar (Wiseana cervinata). In: Prestige RA (ed.) Proc. 6th Australasian Grassl. Invert. Ecol. Conf. AgResearch, Hamilton, New Zealand, pp 331–339.

    Google Scholar 

  • Cribb J (1987) Australian agriculture: The complete reference on rural industry. Morescope for the National Farmers Federation, Camberwell, Victoria, Australia.

    Google Scholar 

  • Dudas B, Woodfield DR, Tong PM, Nicholls MF, Cousins GR, Burgess R, White DWR, Beck DL, Lough TJ, Forster RLS (1998) Estimating the agronomic impact of white clover mosaic virus on white clover performance in the North Island of New Zealand. New Zealand J. Agr Res 41: 171–178.

    Article  Google Scholar 

  • East R, Pottinger RP (1984) The cost of pasture pests. New Zealand J Agr Sci 18: 136–140.

    Google Scholar 

  • Esruch JJ, Warren GW, Mullins MA, Nye G J, Craig JA, Koziel MG (1997) Transgenic plants: an emerging approach to pest control. Nat Biotech 15: 137–141.

    Article  Google Scholar 

  • Forster RLS, Bevan MW, Harbison SA, Gardner RC (1988) The complete nucleotide sequence of the potexvrus white clover mosaic virus. Nucl Acids Res 16: 291–303.

    Article  PubMed  CAS  Google Scholar 

  • Jongsma MA, Bakker P, Peters J, Bosch D, Stiekema WJ (1995) Adaptation of Spodoptera exigua larvae to plant proteinase inhibitors by induction of gut proteinase activity insensitive to inhibition. PNAS (USA) 92: 8041–8085.

    Article  CAS  Google Scholar 

  • McManus MT, Burgess EPJ, Philip B, Watson LM, Laing WA, Voisey CR, White DWR (1999) Expression of the soybean (Kunitz) trypsin inhibitor in transgenic tobacco: Effects on larval development of Spodoptera litura. Transgenic Research 8: 383–395.

    Article  CAS  Google Scholar 

  • Osbourne JK, Watts JW, Beachy RN, Wilson TMA (1989) Evidence that nucleocapsid dissassembly and a later step in virus replication are inhibited in transgenic tobacco expressing TMV coat protein. Virology 172: 370–373.

    Article  Google Scholar 

  • Peferoen M (1997) Progress and prospects for field use of BT genes in crops. Trends Biotech 15:173–177.

    Article  CAS  Google Scholar 

  • Perlak FJ, Fuch RL, Dean DA, McPherson SL, Fischoff DA (1991) Modification of the coding sequence enhances plant expression of insect control proteins. PNAS (USA) 88: 3324–3328.

    Article  CAS  Google Scholar 

  • Roush RT (1994) Managing pests and their resistance to Bacillus thuringiensis: can transgenic crops be better than sprays? Biocontrol Sci & Tech 4: 501–506.

    Article  Google Scholar 

  • Scott A, Woodfield D, White DWR (1998) Allelic composition and genetic background effects on transgene expression and inheritance in white clover. Molecular Breeding 4: 479–490.

    Article  CAS  Google Scholar 

  • Smith HA, Swaney SL, Parks TD, Wernsman EA, Dougherty WG (1994) Transgenic plant virus resistance mediated by untranslatable sense RNAs Plant Cell 6: 1441–1453.

    CAS  Google Scholar 

  • Voisey CR, White DWR, Dudas B, Appleby RD, Ealing PM, Scott AG (1994) Agrobacterium-mediated transformation of white clover using direct shoot organogenesis. Plant Cell Rep 13: 309–314.

    Google Scholar 

  • Voisey CR, White DWR, McGregor PG, Wigley PJ, Chilcott CN (1993) Transformation of white clover with Bt genes. In: Ackhurst RJ (ed) Proc 2nd Canberra Meeting on Bacillus thuriniensis. CSIRO, Division Entomolgy, Canberra, Australia, pp 75–83.

    Google Scholar 

  • Voisey CR, White DWR, Wigley PJ, Chilcott CN, McGregor PG, Woodfield DR (1994) Release of transgenic white clover plants expressing Bacillus thuringiensis genes: An ecological perspective. Biocontrol Sci & Tech 4: 475–481.

    Article  Google Scholar 

  • Watson RN, Skipp RA, Barratt BIP (1989) Initiatives in pest and disease control in New Zealand towards improving legume production and persistence. In: Marten GC, Matches AG, Barnes RF, Brougham RW, Clements RJ, Sheath GW (eds) Persistence of forage legumes. American Soc Agron, CSSA, SSSA, Madison, Wisconsin, USA, pp 441–464.

    Google Scholar 

  • White DWR (1999) Transgenic white clover (Trifolium repens). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Springer-Verlag, Berlin, Heidelberg, pp360–372.

    Google Scholar 

  • White DWR, Greenwood D (1987) Transformation of the forage legume Trifolium repens L. using binary Agrobacterium vectors. Plant Mol Biol 8: 461–469.

    Article  CAS  Google Scholar 

  • White DWR, Voisey CR (1994) Prolific direct plant regeneration from cotyledons of white clover. Plant Cell Rep 13: 303–308.

    Article  CAS  Google Scholar 

  • White DWR, Woodfield DR, Dudas B, Forster RLS, Beck DL (2000) White clover molecular genetics. In: Janick J (ed) Plant breeding reviews. John Wiley & Sons, Inc. vol 17, pp 191–223.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Plant Molecular Genetics Lab. AgResearch, Private Bag, 11008, Palmerston North, New Zealand

    C. R. Voisey, B. Dudas, R. Biggs & D. W. R. White

  2. HortResearch, Private Bag, 92169, Auckland, New Zealand

    E. P. J. Burgess, T. J. Lough, D. L. Beck & R. L. S. Forster

  3. BioDiscovery Ltd, 24 Belfour Road, Parnell, Auckland, New Zealand

    P. J. Wigley

  4. Landcare Research, Private Bag, 11052, Palmerston North, New Zealand

    P. G. McGregor

Authors
  1. C. R. Voisey
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  2. B. Dudas
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  3. R. Biggs
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  4. E. P. J. Burgess
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  5. P. J. Wigley
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  6. P. G. McGregor
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  7. T. J. Lough
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  8. D. L. Beck
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  9. R. L. S. Forster
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  10. D. W. R. White
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Editor information

Editors and Affiliations

  1. Plant Biotechnology Centre, Agriculture Victoria, Melbourne, Australia

    German Spangenberg

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© 2001 Springer Science+Business Media Dordrecht

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Voisey, C.R. et al. (2001). Transgenic Pest and Disease Resistant White Clover Plants. In: Spangenberg, G. (eds) Molecular Breeding of Forage Crops. Developments in Plant Breeding, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9700-5_14

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  • DOI: https://doi.org/10.1007/978-94-015-9700-5_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5676-4

  • Online ISBN: 978-94-015-9700-5

  • eBook Packages: Springer Book Archive

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