Skip to main content

Enzymes as tools for structural studies of pectins

  • Chapter
Bioactive Carbohydrate Polymers

Part of the book series: Proceedings of the Phythochemical Society of Europe ((PPSE,volume 44))

Abstract

The structure elucidation of pectic polysaccharides has obtained new momentum and new dimensions by the increasing availability of pure, well characterised pectin modifying or depolymerizing enzymes and the discovery of a new class of enzymes active towards rhamnogalacturonan structures. They provide us with fragments which fit better in the range of modern NMR and mass spectrometric techniques. Homogalacturonan degrading enzymes give valuable information on the distribution of substituents along the galaturonan backbone.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Voragen, A.G.J., Pilnik, W., Thibault, J.-F., Axelos, M.A.V. & Renard C.M.G.C. (1995) In Food polysaccharides and their applications, ed. Stephen, A.M., Marcel Dekker, New York, pp. 287–339

    Google Scholar 

  2. De Vries, J.A. (1988) In Gums and Stabilizers for the Food Industry 4, eds. Philips G.O., Wedlock, D.J. & Williams, P.A., IRL Press, Oxford, pp. 25–29f

    Google Scholar 

  3. O’Neill, M., Albersheim, P., & Darvill, A.G. (1990) In Methods in Plant Biochemistry, Carbohydrates vol. 2, ed. Dey P.M., Academic, London, pp. 415–441

    Google Scholar 

  4. Schols, H.A., Bakx, E.J., Schipper, D. & Voragen A.G.J. (1995a) Carbohydr. Res., 279, 265–279

    Article  CAS  Google Scholar 

  5. Schols, H.A. Vierhuis, E. Bakx, E.J. and Voragen. A.G.J. (1995b) Carbohydr. Res., 275, 343–360

    Article  PubMed  CAS  Google Scholar 

  6. Albersheim, P., Darvill, A.G., O’Neill, M.A., Schols, H.A. & Voragen, A.G.J. (1996) In Progress in Biotechnology 14, Pectins and Pectinases, eds. Visser, J. & Voragen A.G.J., Elsevier, Amsterdam, pp. 47–55

    Chapter  Google Scholar 

  7. De Vries, J.A., Rombouts, F.M., Voragen, A.G.J. & Pilnik, W. (1982) Carbohydr. Polym., 2, 25–33

    Article  Google Scholar 

  8. Schols, H.A., & Voragen, A.G.J. (1996) In Progress in Biotechnology 14, Pectins and Pectinases, eds. Visser, J. & Voragen A.G.J., Elsevier, Amsterdam, pp. 3–19

    Chapter  Google Scholar 

  9. Beldman, G., Mutter, M., Searle-van Leeuwen, M.J.F., van den Broek, L.A.M., Schols, H.A. & Voragen, A.G.J. (1996a) In Progress in Biotechnology 14, Pectins and Pectinases, eds. Visser, J. & Voragen A.G.J., Elsevier, Amsterdam, pp. 231–245

    Chapter  Google Scholar 

  10. Meeuwsen, P.J.A. et al. (1999) Novel endo-xylogalacturonase, Patent WO-2895-N73992A

    Google Scholar 

  11. Pilnik, W. & Voragen, A.G.J. (1991) In Food Enzymology vol. 1, ed. Fox, P.F., Elsevier, London, pp. 303–336

    Google Scholar 

  12. Doco, T., Williams, P., Vidal, S. & Pellerin, P. (1997) Carbohydr. Res. 297 181–186

    Article  PubMed  CAS  Google Scholar 

  13. Beldman, G. et al (1996b) Biotechnol. Lett. 18, 707–712

    Article  CAS  Google Scholar 

  14. Mutter, M. (1997) New rhamnogalacturonan degrading enzymes from Aspergillus aculeatus. PhD Thesis, Wageningen Agricultural University

    Google Scholar 

  15. Mutter, M., Beldman, G., Schols, H.A. & Voragen, A.G.J. (1994) Plant Physiol., 106, 241–250

    Article  PubMed  CAS  Google Scholar 

  16. Mutter, M., Colquhoun, I.J., Schols, H.A., Beldman, G. & Voragen, A.G.J. (1996a) Plant Physiot. 110, 73–77

    Article  CAS  Google Scholar 

  17. Mutter, M., Renard, C.M.G.C., Beldman, G., Schols, H.A. & Voragen, A.G.J. (1996b) In Progress in Biotechnology 14, Pectins and Pectinases, eds. Visser, J. & Voragen A.G.J., Elsevier, Amsterdam, pp. 263–274.

    Chapter  Google Scholar 

  18. Searle-van Leeuwen, M.J.F., van den Broek, L.A.M., Schols, H.A., Beldman, G. & Voragen A. G. J. (1992) Appl. Microbiol. Biotechnol, 38, 347–349

    Article  CAS  Google Scholar 

  19. Van der Vlugt-Bergmans, C.J.B., Meeuwsen, P.J.A., Voragen, A.G.J. & van Ooyen, A.J.J. (1999), Biochim. Biophys. Acta, submitted

    Google Scholar 

  20. Keegstra, K., Talmadge, K.W., Bauer, W.D. & Albersheim, P. (1973) Plant Physiol. 51 188–197

    Article  PubMed  CAS  Google Scholar 

  21. Schols, HA., Posthumus, M.A. & Voragen, A.G.J. (1990) Carbohydr. Res , 206, 117–129

    Article  CAS  Google Scholar 

  22. De Vries, J.A., Rombouts, F.M., Voragen, A.G.J. & Pilnik, W. (1983a) Carbohydr. Polym. 3, 245–258

    Article  Google Scholar 

  23. De Vries, J.A., Rombouts, F.M., Voragen, A.G.J. & Pilnik, W. (1983b) Carbohydr. Polym. 4, 89–10

    Google Scholar 

  24. Ros, J.M., Schols, H.A. & Voragen, A.G.J. (1996) Carbohydr. Res. 282, 271–284

    Article  CAS  Google Scholar 

  25. Ros, J.M., Schols, H.A. & Voragen, A.G.J. (1998) Carbohydr. Polymers, 37, 159–166f

    Article  CAS  Google Scholar 

  26. Schols, H.A. & Voragen, A.G.J. (1994) Carbohydr. Res , 256, 83–95

    Article  CAS  Google Scholar 

  27. Schols, HA., Voragen, A.G.J. & Colquhoun, I.J. (1994) Carbohydr. Res , 256, 97–111

    Article  PubMed  CAS  Google Scholar 

  28. Weightman, R.M. Renard, C.M.G.C. & Thibault, J.-F. (1994) Carbohydr. Polym. 24, 139–148

    Article  CAS  Google Scholar 

  29. Yu, L. & Mort, A.J. (1996) In Progress in Biotechnology 14, Pectins and Pectinases, eds. Visser, J. & Voragen A.G.J., Elsevier, Amsterdam, pp. 79–88

    Chapter  Google Scholar 

  30. Huisman, M.M.H.,Schols, H.A. & Voragen, A.G.J.(1999) Carbohydr Polym. 38, 299–307

    Article  CAS  Google Scholar 

  31. Yamada, H. & Kiyohara H. (1999) In Immunomodulatory Agents frorn Plants, ed. Wagner, H., Birhhäuser Verlag Basel, pp 161–202

    Chapter  Google Scholar 

  32. Samuelsen, A.B., Paulsen, B.S., Wold, J.K., Otsuko, H., Kiyohara, H., Yamada, H. & Knutsen, S.H. (1996) Carbohydr. Polym. 30, 37–44

    Article  CAS  Google Scholar 

  33. Taylor, A.J. (1982) Carbohydr. Polym. 2, 9–17

    Article  CAS  Google Scholar 

  34. Tuerena, C.E., Taylor, A.J. & Mitchell, J.R. (1981) Carbohydr. Polym. 2, 193–203

    Article  Google Scholar 

  35. Tuerena, C.E., Taylor, A.J. & Mitchell, J.R. (1984) J. Sci. Food Agric., 35, 797–804

    Article  CAS  Google Scholar 

  36. De Vries, J.A., Hansen, M., Søderberg, J., Glahn, J. P.E. & Pedersen J.K. (1986) Carbohydr. Polym., 6, 165–176

    Article  Google Scholar 

  37. Kiyohara, H., Cyong, J.-C. & Yamada, H. (1988) Carbohydr. Res. 182, 259–275

    Article  PubMed  CAS  Google Scholar 

  38. Kiyohara, H. & Yamada, H.(1994) Carbohydr. Polym. 25, 117–122

    Article  CAS  Google Scholar 

  39. Kravtchenko, T.P., Berth, G., Voragen, A.G.J. & Pilnik, W. (1992a) Carbohydr. Polym. 18, 253–263

    Article  CAS  Google Scholar 

  40. Kravtchenko, T.P., Voragen, A.G.J. & Pilnik, W. (1992b) Carbohydr. Polym., 18, 17–25

    Article  CAS  Google Scholar 

  41. Kravtchenko, T.P., Voragen, A.G.J. & Pilnik, W. (1992c) Carbohydr. Polym. 19,115–124

    Article  CAS  Google Scholar 

  42. Kravtchenko, T.P., Penci, M., Voragen, A.G.J. & Pilnik, W. (1993) Carbohydr. Polym. 20, 195–205

    Article  CAS  Google Scholar 

  43. Daas, P.J.H., Arisz, P.W., Schols, H.A., De Ruiter, G.A. & Voragen, A.G.J. (1998) Anal. Biochem. 257, 195–202

    Article  PubMed  CAS  Google Scholar 

  44. Körner, R., Limberg, G., Dalgaard Mikkelsen, J. & Roepstorff, P. (1998) J. Mass. Spectrom. 33, 836–842

    Article  PubMed  Google Scholar 

  45. Daas, P.J.H., Meyer-Hansen, K., Schols, H.A., De Ruiter, G.A. & Voragen, A.G.J. (1999) Carbohydr. Res., in press

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Voragen, A.G.J., Daas, P.J.H., Schols, H.A. (2000). Enzymes as tools for structural studies of pectins. In: Paulsen, B.S. (eds) Bioactive Carbohydrate Polymers. Proceedings of the Phythochemical Society of Europe, vol 44. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9572-8_11

Download citation

  • DOI: https://doi.org/10.1007/978-94-015-9572-8_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5379-4

  • Online ISBN: 978-94-015-9572-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics