Abstract
Polymerase Chain Reaction is a very common technic used in most laboratories to identify bacteria, fungi, viruses, or DNA encoding for a toxin, a protein. In this presentation, we will first review some examples of its uses in medical laboratories and then focus on its use for detection and identification in environmental samples.
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Pershing, H. (1993) Diagnostic molecular microbiology, principles and applications edited by D.H. Pershing. T.F. Smith. FC. Tenover, and T.J. White, SFM, Mayo Foundation.
Moseley, S.L., I. Hug, A.R. Alim, M. So, M. Samadpour-Motalebi, and S. Falkow (1980) Detection of enterotoxigenic Escherichia coli by DNA colony hybridization. J. Infect. Dis. 142: 892–898.
Ramisse, V., G. Patra, H. Garrigue, J.-L. Guesdon, and M. Mock (1996) Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA. FEMS Microbiol. Lett. 145: 9–16.
Gibbs, R.A., P-N. Nguyen, and C.T. Caskey (1989) Detection of single DNA base differences by competitive oligonucleotide priming. Nucleic Acids Res. 17: 2437–2448.
Jackson, P.J., E.A. Walthers, A.S. Kalif, K.L. Richmond, D.M. Adair, K.K. Hill, C.R. Kuske, G.L. Andersen, K.H. Wilson, M.E. Hugh-Jones, and P. Keim (1997) Characterization of the Variable-Number Tandem Repeats in vvrA from Different Bacillus anthracis Isolates. Appli. Environ. Microbiol. 63 (4): 1400–1405.
Vincent, L., G. Patra, J. Therasse, and P. Gareil (1996) Quantitation of polymerase chain reaction -amplified DNA fragments on capillary electrophoresis and laser-induced fluorescence detection. Electrophoresis 17: 512–517.
Patra, G., P. Sylvestre, V. Ramisse, J. Therasse, and J.-L. Guesdon (1996) Isolation of a specific chromosomic DNA sequence of Bacillus anthracis and its possible use in diagnosis. FEMS Immunol. Med. Microbiol. 15: 223–231.
Berthier, M., J.-L. Fauchere, J. Perrin, B. Grignon, and D. Oriot (1996) Fulminant meningitis due to Bacillus anthracis in an 11 year old girl during Ramadan. Lancet 347 (9004): 828.
Keim, P., A. Kalif, J. Schupp, K. Hill, S.E. Travis, K. Richmond, D.M. Adair, M. Hugh-Jones, CR. Kuske, and P. Jackson (1997) Molecular Evolution and Diversity in Bacillus anthracis as Detected by Amplified Fragment Length Polymorphism Markers. J. Bacteriol. 179 (3): 818–824.
Ramisse, V. (1997) Personnel Communication.
Felske, A., B. Engelen, U. Nubel, and H. Backhaus (1996) Direct Ribosome Isolation from Soil to Extract Bacterial rRNA for Community Analysis. Appl. Environ. Microbiol. 62 (11): 4162–4167.
Patra, G., P. Sylvestre, V. Ramisse, J. Therasse, and J.-L. Guesdon (1997) Characterization of Bacillus anthracis strains by rDNA Gene Restriction Patterns (Manuscript in preparation).
Patra, G. and J.-L. Guesdon (1997) (Unpublished data).
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© 2000 Springer Science+Business Media Dordrecht
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Garrigue, H., Patra, G., Ramisse, V. (2000). Use of PCR for Identification and Detection of Biological Agents. In: Stopa, P.J., Bartoszcze, M.A. (eds) Rapid Methods for Analysis of Biological Materials in the Environment. NATO ASI Series, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9534-6_21
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DOI: https://doi.org/10.1007/978-94-015-9534-6_21
Publisher Name: Springer, Dordrecht
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