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Characterization of Native Actin-Binding Proteins from Pollen

Myosin and the actin-bundling proteins, 135-ABP and 115-ABP

  • Chapter
Actin: A Dynamic Framework for Multiple Plant Cell Functions

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 89))

Abstract

A myosin (170 kDa) and two types of actin-bundling proteins (135-ABP and 115-ABP) were isolated from pollen tubes of lily, Lilium longiflorum. The myosin consists of a 170-kDa heavy chain with calmodulin light chain. On the basis of immunolocalization studies using antiserum against the 170-kDa heavy chain and the analysis of the sliding velocity of F-actin in vitro, this myosin was suggested to be a motor responsible for cytoplasmic streaming. The motile activity of 170-kDa myosin was suppressed by Ca2+ through the calmodulin light chain. On the other hand, both ABPs arranged actin filaments into bundles that serve as tracks for cytoplasmic streaming. The bundling activity of 135-ABP was inhibited by Ca2+-calmodulin. Physiological studies revealed that Ca2+ induces not only the inactivation of motor activity for cytoplasmic streaming but also causes the fragmentation and disorganization of actin bundles in pollen tubes, which should be correlated with the regulation of cytoplasmic streaming by Ca2+. Hence, the Ca2+ sensitivity in the activities of 170-kDa myosin and 135-ABP, acting through calmodulin, is suggested to be a molecular basis for the Ca2+ regulation of cytoplamsic streaming in pollen tubes.

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  1. Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, Hyogo, 678-1297, Japan

    Etsuo Yokota & Teruo Shimmen

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  1. Etsuo Yokota
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  2. Teruo Shimmen
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C. J. Staiger F. Baluška D. Volkmann P. W. Barlow

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Yokota, E., Shimmen, T. (2000). Characterization of Native Actin-Binding Proteins from Pollen. In: Staiger, C.J., Baluška, F., Volkmann, D., Barlow, P.W. (eds) Actin: A Dynamic Framework for Multiple Plant Cell Functions. Developments in Plant and Soil Sciences, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9460-8_6

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