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Steedman’s Wax for F-Actin Visualization

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Actin: A Dynamic Framework for Multiple Plant Cell Functions

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 89))

Abstract

Actin filaments are visualised by means of indirect immunofluorescence in plant tissues that were fixed in formaldehyde, embedded in low-melting polyester wax and sectioned on a microtome. The technique described here avoids usage of detergents and organic solvents and is also compatible with immunolocalization of many other antigens.

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Author information

Authors and Affiliations

  1. Institute of Plant Molecular Biology, Czech Academy of Sciences, České Budějovice, CZ-37005, Czech Republic

    Stanislav Vitha

  2. Department of Biochemistry, University of Nevada, Reno, NV 89557, USA

    Stanislav Vitha

  3. Botanisches Institut, Universität Bonn, Kirschallee 1, D-53115, Bonn, Germany

    František Baluška & Dieter Volkmann

  4. Department of Plant Physiology, Comenius University, SK-84215, Bratislava, Slovakia

    Jan Jasik

  5. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS41 9AF, UK

    Peter W. Barlow

Authors
  1. Stanislav Vitha
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  2. František Baluška
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  3. Jan Jasik
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  4. Dieter Volkmann
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  5. Peter W. Barlow
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Editor information

C. J. Staiger F. Baluška D. Volkmann P. W. Barlow

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© 2000 Springer Science+Business Media Dordrecht

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Vitha, S., Baluška, F., Jasik, J., Volkmann, D., Barlow, P.W. (2000). Steedman’s Wax for F-Actin Visualization. In: Staiger, C.J., Baluška, F., Volkmann, D., Barlow, P.W. (eds) Actin: A Dynamic Framework for Multiple Plant Cell Functions. Developments in Plant and Soil Sciences, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9460-8_35

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  • DOI: https://doi.org/10.1007/978-94-015-9460-8_35

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5504-0

  • Online ISBN: 978-94-015-9460-8

  • eBook Packages: Springer Book Archive

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