Skip to main content
SpringerLink
Log in

Profilin

  • Chapter
Actin: A Dynamic Framework for Multiple Plant Cell Functions

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 89))

Abstract

Profilin has complex effects on the actin cytoskeleton; it can bind to monomeric actin and thereby inhibit polymerization, yet under other conditions it stimulates polymerization. Profilin also binds to polyphosphoinositides and proline-rich proteins which are implicated in signaling events that result in reorganization of the actin cytoskeleton. Plant profilins share many of the same biochemical properties and are structurally similar to non-plant profilins. Microinjection of profilin into plant cells inhibits cytoplasmic streaming, tip growth and cytokinesis through the depolymerization of actin microfilaments. Furthermore, measurements of profilin and actin levels in pollen are consistent with profilin sequestering the bulk of the actin monomer pool. Together these results suggest that profilin serves to sequester actin in plant cells. This simple model is complicated by the fact that there are two functionally-distinct classes of profilin, at least in maize, and in some cells both classes of profilin are expressed in the same cytoplasm. One class of profilin appears to have a high affinity for pollen actin and poly-L-proline, whereas the other class has a 2–4-fold lower affinity for actin and a 2-fold lower affinity for poly-L-proline.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Asturias JA, Arilla MC, Bartolomé B, Martínez J, Martínez A and Palacios R (1997a) Sequence polymorphism and structural analysis of timothy grass pollen profilin allergen (Phl p 11). Biochim Biophys Acta 1352: 253–257

    Article  PubMed  CAS  Google Scholar 

  • Asturias JA, Arilla MC, Gómez-Bayón N, Aguirre M, Martinez A, Palacios R and Martinez J (1998) Cloning and immunological characterization of the allergen Hel a 2 (profilin) from sunflower pollen. Mol Immunol 35: 469–478

    Article  PubMed  CAS  Google Scholar 

  • Asturias JA, Arilla MC, Gómez-Bayón N, Martinez J, Martinez A and Palacios R (1997b) Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen. J Allergy Clin Immunol 100: 365–372

    Article  PubMed  CAS  Google Scholar 

  • Asturias JA, Arilla MC, Gómez-Bayón N, Martinez J, Martinez A and Palacios R (1997c) Cloning and high level expression of Cynodon dactylon (Bermuda grass) pollen profilin (Cyn d 12) in Escherichia coli: purification and characterization of the allergen. Clin Exp Allergy 27: 1307–1313

    Article  PubMed  CAS  Google Scholar 

  • Ayscough KR (1998) In vivo functions of actin-binding proteins. Curr Opin Cell Biol 10: 102–111

    Article  PubMed  CAS  Google Scholar 

  • Balasubramanian MK, Feoktistova A, McCollum D and Gould KL (1996) Fission yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin function. EMBO J 15: 6426–6437

    PubMed  CAS  Google Scholar 

  • Ballweber E, Giehl K, Hannappel E, Huff T, Jockusch BM and Mannherz HG (1998) Plant profilin induces actin polymerization from actin: β-thymosin complexes and competes directly with β-thymosins and with negative co-operativity with DNase I for binding to actin. FEBS Lett 425: 251–255

    Article  PubMed  CAS  Google Scholar 

  • Braun M, Baluska F, von Witsch M and Menzel D (1999) Redistribution of actin, profilin and phosphatidylinositol-4,5-bisphosphate in growing and maturing root hairs. Planta 209: 435–443

    Article  PubMed  CAS  Google Scholar 

  • Bubb MR, Baines IC and Korn ED (1998) Localization of actobindin, profilin I, profilin II, and phosphatidylinositol-4,5-bisphosphate (PIP2) in Acanthamoeba castellana. Cell Motil Cytoskeleton 39: 134–146

    Article  PubMed  CAS  Google Scholar 

  • Cao L-G, Babcock GG, Rubenstein PA and Wang Y-L (1992) Effects of profilin and profilactin on actin structure and function in living cells. J Cell Biol 117: 1023–1029

    Article  PubMed  CAS  Google Scholar 

  • Carlier M-F (1998) Control of actin dynamics. Curr Opin Cell Biol 10: 45–51

    Article  PubMed  CAS  Google Scholar 

  • Carlsson L, Nyström L-E, Sundkvist I, Markey F and Lindberg U (1977) Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells. J Mol Biol 115: 465–483

    Article  PubMed  CAS  Google Scholar 

  • Castrillon DH and Wasserman SA (1994) diaphanous is required for cytokinesis in Drosophila and shares domains of similarity with the products of the limb deformity gene. Development 120: 3367–3377

    PubMed  CAS  Google Scholar 

  • Cedergren-Zeppezauer ES, Goonesekere NCW, Rozycki MD, Myslik JC, Dauter Z, Lindberg U and Schutt CE (1994) Crystallization and structure determination of bovine profilin at 2.0 Å resolution. J Mol Biol 240: 459–475

    Article  PubMed  CAS  Google Scholar 

  • Chang F, Drubin D and Nurse P (1997) cdcl2p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin. J Cell Biol 137: 169–182

    Article  PubMed  CAS  Google Scholar 

  • Christensen HEM, Ramachandran S, Tan C-T, Surana U, Dong C-H and Chua N-H (1996) Arabidopsis profilins are functionally similar to yeast profilins: identification of a vascular bundle-specific profilin and a pollen-specific profilin. Plant J 10: 269–279

    Article  PubMed  CAS  Google Scholar 

  • Clarke SR, Staiger CJ, Gibbon BC and Franklin-Tong VE (1998) A potential signaling role for profilin in pollen of Papaver rhoeas. Plant Cell 10: 967–979

    PubMed  CAS  Google Scholar 

  • Cossart P (1995) Actin-based bacterial motility. Curr Opin Cell Biol 7: 94–101

    Article  PubMed  CAS  Google Scholar 

  • Domke T, Federau T, Schlüter K, Giehl K, Valenta R, Schomburg D and Jockusch BM (1997) Birch pollen profilin: structural organization and interaction with poly-(L-proline) peptides as revealed by NMR. FEBS Lett 411: 291–295

    Article  PubMed  CAS  Google Scholar 

  • Drøbak BK, Watkins PAC, Valenta R, Dove SK, Lloyd CW and Staiger CJ (1994) Inhibition of plant plasma membrane phosphoinositide phospholipase C by the actin-binding protein, profilin. Plant J 6: 389–400

    Article  Google Scholar 

  • Eads JC, Mahoney NM, Vorobiev S, Bresnick AR, Wen KK, Rubenstein PA, Haarer BK and Almo SC (1998) Structure determination and characterization of Saccharomyces cerevisiae profilin. Biochemistry 37: 11171–11181

    Article  PubMed  CAS  Google Scholar 

  • Evangelista M, Blundell K, Longtine MS, Chow CJ, Adames N, Pringle JR, Peter M and Boone C (1997) Bni 1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis. Science 276: 118–122

    Article  PubMed  CAS  Google Scholar 

  • Fedorov AA, Ball T, Mahoney NM, Valenta R and Almo SC (1997) The molecular basis for allergen cross-reactivity: crystal structure and IgE-epitope mapping of birch pollen profilin. Structure 5: 33–45

    Article  PubMed  CAS  Google Scholar 

  • Fedorov AA, Magnus KA, Graupe MH, Lattman EE, Pollard TD and Almo SC (1994a) X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates. Proc Natl Acad Sci USA 91: 8636–8640

    Article  PubMed  CAS  Google Scholar 

  • Fedorov AA, Pollard TD and Almo SC (1994b) Purification, characterization and crystallization of human platelet profilin expressed in Escherichia coli. J Mol Biol 241: 480–482

    Article  PubMed  CAS  Google Scholar 

  • Finkel T, Theriot JA, Dise KR, Tomaselli GF and Goldschmidt-Clermont PJ (1994) Dynamic actin structures stabilized by profilin. Proc Natl Acad Sci USA 91: 1510–1514

    Article  PubMed  CAS  Google Scholar 

  • Frazier JA and Field CM (1997) Actin cytoskeleton: Are FH proteins local organizers? Curr Biol 7: R414–R417

    Article  PubMed  CAS  Google Scholar 

  • Gibbon BC, Kovar DR and Staiger CJ (1999) Latrunculin B has different effects on pollen germination and tube growth. Plant Cell 11: 2349–2363

    PubMed  CAS  Google Scholar 

  • Gibbon BC, Ren H and Staiger CJ (1997) Characterization of maize (Zea mays) pollen profilin function in vitro and in live cells. Biochem J 327: 909–915

    PubMed  CAS  Google Scholar 

  • Gibbon BC, Zonia LE, Kovar DR, Hussey PJ and Staiger CJ (1998) Pollen profilin function depends on interaction with proline-rich motifs. Plant Cell 10: 981–993. [A printed correction appears in Plant Cell 11, 1603]

    PubMed  CAS  Google Scholar 

  • Giehl K, Valenta R, Rothkegel M, Ronsiek M, Mannherz H-G and Jockusch BM (1994) Interaction of plant profilin with mammalian actin. Eur J Biochem 226: 681–689

    Article  PubMed  CAS  Google Scholar 

  • Gieselmann R, Kwiatkowski DJ, Janmey PA and Witke W (1995) Distinct biochemical characteristics of the two human profilin isoforms. Eur J Biochem 229: 621–628

    Article  PubMed  CAS  Google Scholar 

  • Giesemann T, Rathke-Hartlieb S, Rothkegel M, Bartsch JW, Buchmeier S, Jockusch BM and Jockusch H (1999) A role for polyproline motifs in the spinal muscular atrophy protein SMN: profilins bind to and colocalize with SMN in nuclear gems. J Biol Chem 274: 37908–37914

    Article  PubMed  CAS  Google Scholar 

  • Goldschmidt-Clermont PJ, Furman MI, Wachsstock D, Safer D, Nachmias VT and Pollard TD (1992) The control of actin nucleotide exchange by thymosin β4 and profilin. A potential regulatory mechanism for actin polymerization in cells. Mol Biol Cell 3: 1015–1024

    PubMed  CAS  Google Scholar 

  • Goldschmidt-Clermont PJ, Kim JW, Machesky LM, Rhee SG and Pollard TD (1991) Regulation of phospholipase C-γ1 by profilin and tyrosine phosphorylation. Science 251: 1231–1233

    Article  PubMed  CAS  Google Scholar 

  • Goldschmidt-Clermont PJ, Machesky LM, Baldassare JJ and Pollard TD (1990) The actin-binding protein profilin binds to PIP2 and inhibits its hydrolysis by phospholipase C. Science 247: 1575–1578

    Article  PubMed  CAS  Google Scholar 

  • Guillén G, Valdés-López V, Noguez R, Olivares J, Rodriguez-Zapata LC, Pérez H, Vidali L, Villanueva MA and Sánchez F (1999) Profilin in Phaseolus vulgaris is encoded by two genes (only one expressed in root nodules) but multiple isoforms are generated in vivo by phosphorylation on tyrosine residues. Plant J 19: 497–508

    Article  PubMed  Google Scholar 

  • Haarer BK, Lillie SH, Adams AE, Magdolen V, Bandlow W and Brown SS (1990) Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells. J Cell Biol 110: 105–114

    Article  PubMed  CAS  Google Scholar 

  • Hájková L, Sjögren CB, Korenbaum E, Nordberg P and Karlsson R (1997) Characterization of a mutant profilin with reduced actin-binding capacity: effects in vitro and in vivo. Exp Cell Res 234: 66–77

    Article  PubMed  Google Scholar 

  • Hall A (1994) Small GTP-binding proteins and the regulation of the actin cytoskeleton. Annu Rev Cell Biol 10: 31–54

    Article  PubMed  CAS  Google Scholar 

  • Harris SD, Hamer L, Sharpless KE and Hamer JE (1997) The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity. EMBO J 16: 3474–3483

    Article  PubMed  CAS  Google Scholar 

  • Haugwitz M, Noegel AA, Karakesisoglou J and Schleicher M (1994) Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis and development. Cell 79: 303–314

    Article  PubMed  CAS  Google Scholar 

  • Hess MW, Mittermann I, Luschnig C and Valenta R (1995) Immunocytochemical localisation of actin and profilin in the generative cell of angiosperm pollen: TEM studies on highpressure frozen and freeze-substituted Ledebouria socialis Roth (Hyacinthaceae). Histochemistry 104: 443–451

    Article  PubMed  CAS  Google Scholar 

  • Hess MW and Valenta R (1997) Profilin revealed in pollen nuclei: immuno-electron microscopy of high-pressure frozen Ledebouria socialis Roth (Hyacinthaceae). Sex Plant Reprod 10: 283–287

    Article  CAS  Google Scholar 

  • Holzinger A, Mittermann I, Laffer S, Valenta R and Meindl U (1997) Microinjection of profilins from different sources into the green alga Micrasterias causes transient inhibition of cell growth. Protoplasma 199: 124–134

    Article  Google Scholar 

  • Huang S, McDowell JM, Weise MJ and Meagher RB (1996) The Arabidopsis profilin gene family: evidence for an ancient split between constitutive and pollen-specific profilin genes. Plant Physiol 111: 115–126

    Article  PubMed  CAS  Google Scholar 

  • Imamura H, Tanaka K, Hihara T, Umikawa K, Kamei T, Takahashi K, Sasaki T and Takai Y (1997) Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae. EMBO J 16: 2745–2755

    Article  PubMed  CAS  Google Scholar 

  • Jensen-Jarolim E, Santner B, Leitner A, Grimm R, Scheiner O, Ebner C and Breiteneder H (1998) Bell peppers (Capsicum annuum) express allergens (profilin, pathogenesis-related protein P23 and Bet v 1) depending on the horticultural strain. Int Arch Allergy Immunol 116:103–109

    Article  PubMed  CAS  Google Scholar 

  • Jonckheere V, Lambrechts A, Vandekerckhove J and Ampe C (1999) Dimerization of profilin II upon binding the (GP5)3 peptide from VASP overcomes the inhibition of actin nucleation by profilin II and thymosin β4. FEBS Letters 447: 257–263

    Article  PubMed  CAS  Google Scholar 

  • Kang F, Punch DL and Southwick FS (1999) Profilin promotes barbed-end actin filament assembly without lowering the critical concentration. J. Biol. Chem. 274: 36963–36972

    Article  PubMed  CAS  Google Scholar 

  • Karakesisoglou I, Schleicher M, Gibbon BC and Staiger CJ (1996) Plant profilins rescue the aberrant phenotype of profilin-deficient Dictyostelium cells. Cell Motil Cytoskeleton 34: 36–47

    Article  PubMed  CAS  Google Scholar 

  • Kohno H, Tanaka K, Mino A, Umikawa M, Imamura H, Fujiwara T, Fujita Y, Hotta K, Qadota H, Watanabe T, Ohya Y and Takai Y (1996) Bnilp implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae. EMBO J 15: 6060–6068

    PubMed  CAS  Google Scholar 

  • Korenbaum E, Nordberg P, Björkegren-Sjögren C, Schutt CE, Lindberg U and Karlsson R (1998) The role of profilin in actin polymerization and nucleotide exchange. Biochemistry 37: 9274–9283

    Article  PubMed  CAS  Google Scholar 

  • Kovar DR, Drøbak BK and Staiger CJ (2000) Maize profilin isoforms are functionally distinct. Plant Cell 12: In press

    Google Scholar 

  • Lambrechts A, Van Damme J, Goethals M, Vandekerckhove J and Ampe C (1995) Purification and characterization of bovine profilin IL Actin, poly(L-proline) and inositolphospholipid binding. Eur J Biochem 230: 281–286

    Article  PubMed  CAS  Google Scholar 

  • Lassing I and Lindberg U (1985) Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin. Nature 314: 472–474

    Article  PubMed  CAS  Google Scholar 

  • Lassing I and Lindberg U (1988) Specificity of the interaction between phosphatidylinositol 4,5-bisphosphate and the profilin:actin complex. J Cell Biochem 37: 255–267

    Article  PubMed  CAS  Google Scholar 

  • Liu X and Yen L-F (1992) Purification and characterization of actin from maize pollen. Plant Physiol 99: 1151–1155

    Article  PubMed  CAS  Google Scholar 

  • Loisel TP, Boujemaa R, Pantaloni D and Carlier M-F (1999) Reconstitution of actin-based motility of Listeria and Shigella using pure proteins. Nature 401: 613–616

    Article  PubMed  CAS  Google Scholar 

  • Lu PJ, Shieh WR, Rhee SG, Yin HL and Chen CS (1996) Lipid products of phosphoinositide 3-kinase bind human profilin with high affinity. Biochemistry 35: 14027–14034

    Article  PubMed  CAS  Google Scholar 

  • Machesky LM, Atkinson SJ, Ampe C, Vandekerckhove J and Pollard TD (1994) Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J Cell Biol 127: 107–115

    Article  PubMed  CAS  Google Scholar 

  • Machesky LM and Pollard TD (1993) Profilin as a potential mediator of membrane-cytoskeleton communication. Trends Cell Biol 3: 381–385

    Article  PubMed  CAS  Google Scholar 

  • Mahoney NM, Janmey PA and Almo SC (1997) Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation. Nature Struc Biol 4: 953–960

    Article  CAS  Google Scholar 

  • Manseau L, Calley J and Phan H (1996) Profilin is required for posterior patterning of the Drosophila oocyte. Development 122: 2109–2116

    PubMed  CAS  Google Scholar 

  • McCollum D, Feoktistova A, Morphew M, Balasubramanian M and Gould KL (1996) The Schizosaccharomyces pombe actin-related protein, Arp3, is a component of the cortical actin cytoskeleton and interacts with profilin. EMBO J 15: 6438–6446

    PubMed  CAS  Google Scholar 

  • Meagher RB (1991) Divergence and differential expression of actin gene families in higher plants. Int Rev Cytol 125: 139–163

    Article  PubMed  CAS  Google Scholar 

  • Meagher RB, McKinney EC and Kandasamy MK (1999a) Isovariant dynamics expand and buffer the responses of complex systems: The diverse plant actin gene family. Plant Cell 11:995–1005

    PubMed  CAS  Google Scholar 

  • Meagher RB, McKinney EC and Vitale AV (1999b) The evolution of new structures: clues from plant cytoskeletal genes. Trends Genet 15: 278–284

    Article  PubMed  CAS  Google Scholar 

  • Metzler WJ, Farmer BT, Constantine KL, Friedrichs MS, Lavoie T and Mueller L (1995) Refined solution structure of human profilin I. Prot Sci 4: 450–459

    Article  CAS  Google Scholar 

  • Mittermann I, Heiss S, Kraft D, Valenta R and Heberle-Bors E (1996) Molecular characterization of profilin isoforms from tobacco (Nicotiana tabacum) pollen. Sex Plant Reprod 9: 133–139

    Article  Google Scholar 

  • Mittermann I, Swoboda I, Pierson E, Eller N, Kraft D, Valenta R and Heberle-Bors E (1995) Molecular cloning and characterization of profilin from tobacco (Nicotiana tabacum): increased profilin expression during pollen maturation. Plant Mol Biol 27: 137–146

    Article  PubMed  CAS  Google Scholar 

  • Mullins RD, Heuser JA and Pollard TD (1998a) The interaction of arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments. Proc Natl Acad Sci USA 95: 6181–6186

    Article  PubMed  CAS  Google Scholar 

  • Mullins RD, Kelleher JF, Xu J and Pollard TD (1998b) Arp2/3 complex from Acanthamoeba binds profilin and cross-links actin filaments. Mol Biol Cell 9: 841–852

    PubMed  CAS  Google Scholar 

  • Ostrander DB, Gorman JA and Carman GM (1995) Regulation of profilin localization in Saccharomyces cerevisiae by phosphoinositide metabolism. J Biol Chem 270: 27045–27050

    Article  PubMed  CAS  Google Scholar 

  • Pantaloni D and Carlier M-F (1993) How profilin promotes actin filament assembly in the presence of thymosin β4. Cell 75: 1007–1014

    Article  PubMed  CAS  Google Scholar 

  • Perelroizen I, Didry D, Christensen H, Chua N-H and Carlier M-F (1996) Role of nucleotide exchange and hydrolysis in the function of profilin in actin assembly. J Biol Chem 271: 12302–12309

    Article  PubMed  CAS  Google Scholar 

  • Petrella EC, Machesky LM, Kaiser DA and Pollard TD (1996) Structural requirements and thermodynamics of the interaction of proline peptides with profilin. Biochemistry 35: 16535–16543

    Article  PubMed  CAS  Google Scholar 

  • Purich DL and Southwick FS (1997) ABM-1 and ABM-2 homology sequences: consensus docking sites for actin-based motility defined by oligoproline regions in Listeria ActA surface protein and human VASP. Biochem Biophys Res Comm 231: 686–691

    Article  PubMed  CAS  Google Scholar 

  • Reinhard M, Giehl K, Abel K, Haffher C, Jarchau T, Hoppe V, Jockusch BM and Walter U (1995) The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. EMBO J 14: 1583–1589

    PubMed  CAS  Google Scholar 

  • Ren H, Gibbon BC, Ashworth SL, Sherman DM, Yuan M and Staiger CJ (1997) Actin purified from maize pollen functions in living plant cells. Plant Cell 9: 1445–1457

    PubMed  CAS  Google Scholar 

  • Rihs HP, Rozynek P, May TK, Welticke B and Baur X (1994) Polymerase chain reaction based cDNA cloning of wheat profilin: a potential plant allergen. Int Arch Allergy Immunol 105: 190–194

    Article  PubMed  CAS  Google Scholar 

  • Rothkegel M, Mayboroda O, Rohde M, Wucherpfennig C, Valenta R and Jockusch BM (1996) Plant and animal profilins are functionally equivalent and stabilize microfilaments in living animal cells. J Cell Sci 109: 83–90

    PubMed  CAS  Google Scholar 

  • Rudd JJ, Franklin FCH and Franklin-Tong VE (1997) Ca2+-independent phosphorylation of a 68 kDa pollen protein is stimulated by the self-incompatibility response in Papaver rhoeas. Plant J 12: 507–514

    Article  CAS  Google Scholar 

  • Rudd JJ, Franklin FCH, Lord JM and Franklin-Tong VE (1996) Increased phosphorylation of a 26-kd pollen protein is induced by the self-incompatibility response in Papaver rhoeas. Plant Cell 8: 713–724

    PubMed  CAS  Google Scholar 

  • Ruhlandt G, Lange U and Grolig F (1994) Profilins purified from higher plants bind to actin from cardiac muscle and to actin from a green alga. Plant Cell Physiol 35: 849–854

    PubMed  CAS  Google Scholar 

  • Schmidt A and Hall MN (1998) Signaling to the actin cytoskeleton. Annu Rev Cell Dev Biol 14: 305–338

    Article  PubMed  CAS  Google Scholar 

  • Schobert C, Gottschalk M, Kovar DR, Staiger CJ, Yoo B-C and Lucas WJ (2000) Characterization of Ricinus communis phloem profilin, RcPRO1. Plant Mol Biol, In press

    Google Scholar 

  • Schutt CE, Myslik JC, Rozycki MD, Goonesekere NCW and Lindberg U (1993) The structure of crystalline profilin-β-actin. Nature 365: 810–816

    Article  PubMed  CAS  Google Scholar 

  • Seiden LA, Kinosian HJ, Estes JE and Gershman LC (1999) Impact of profilin on actin-bound nucleotide exchange and actin polymerization dynamics. Biochemistry 38: 2769–2778

    Article  Google Scholar 

  • Sohn RH, Chen J, Koblan KS, Bray PF and Goldschmidt-Clermont PJ (1995) Localization of a binding site for phosphatidylinositol 4,5-bisphosphate on human profilin. J Biol Chem 270:21114–21120

    Article  PubMed  CAS  Google Scholar 

  • Sohn RH and Goldschmidt-Clermont PJ (1994) Profilin: at the crossroads of signal transduction and the actin cytoskeleton. Bio Essays 16: 465–472

    CAS  Google Scholar 

  • Staiger CJ, Gibbon BC, Kovar DR and Zonia LE (1997) Profilin and actin depolymerizing factor: modulators of actin organization in plants. Trends Plant Sci 2: 275–281

    Article  Google Scholar 

  • Staiger CJ, Goodbody KC, Hussey PJ, Valenta R, Drøbak BK and Lloyd CW (1993) The profilin multigene family of maize: differential expression of three isoforms. Plant J 4: 631–641

    Article  PubMed  CAS  Google Scholar 

  • Staiger CJ, Yuan M, Valenta R, Shaw PJ, Warn RM and Lloyd CW (1994) Microinjected profilin affects cytoplasmic streaming in plant cells by rapidly depolymerizing actin microfilaments. Curr Biol 4: 215–219

    Article  PubMed  CAS  Google Scholar 

  • Thorn KS, Christensen HEM, Shigeta R, Huddler D, Shalaby L, Lindberg U, Chua N-H and Schutt CE (1997) The crystal structure of a major allergen from plants. Structure 5: 19–32

    Article  PubMed  CAS  Google Scholar 

  • Valenta R, Ball T, Vrtala S, Duchêne M, Kraft D and Scheiner O (1994) cDNA cloning and expression of timothy grass (Phleum pratense) pollen profilin in Escherichia coli: comparison with birch pollen profilin. Biochem Biophys Res Comm 199: 106–118

    Article  PubMed  CAS  Google Scholar 

  • Valenta R, Duchêne M, Pettenburger K, Sillaber C, Valent P, Bettelheim P, Breitenbach M, Rumpold H, Kraft D and Scheiner O (1991) Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals. Science 253: 557–560

    Article  PubMed  CAS  Google Scholar 

  • Valenta R, Ferreira F, Grote M, Swoboda I, Vítala S, Duchêne M, Deviller P, Meagher RB, McKinney E, Heberle-Bors E, Kraft D and Scheiner O (1993) Identification of profilin as an actin-binding protein in higher plants. J Biol Chem 268: 22777–22781

    PubMed  CAS  Google Scholar 

  • Vallverdu A, Asturias JA, Arilla MC, Gómez-Bayón N, Martinez A, Martinez J and Palacios R (1998) Characterization of recombinant Mercurialis annua major allergen Mer a 1 (profilin). J Allergy Clin Immunol 101: 363–370

    Article  PubMed  CAS  Google Scholar 

  • Valster AH, Pierson ES, Valenta R, Hepler PK and Emons AMC (1997) Probing the plant actin cytoskeleton during cytokinesis and interphase by profilin microinjection. Plant Cell 9: 1815–1824

    PubMed  CAS  Google Scholar 

  • Vidali L and Hepler PK (1997) Characterization and localization of profilin in pollen grains and tubes of Lilium longiflorum. Cell Motil Cytoskeleton 36: 323–338

    Article  PubMed  CAS  Google Scholar 

  • Vidali L, Pérez HE, López VV, Noguez R, Zamudio F and Sánchez F (1995) Purification, characterization, and cDNA cloning of profilin from Phaseolus vulgaris. Plant Physiol 108:115–123

    Article  PubMed  CAS  Google Scholar 

  • Vinson VK, De La Cruz EM, Higgs HN and Pollard TD (1998) Interactions of Acanthamoeba profilin with actin and nucleotides bound to actin. Biochemistry 37: 10871–10880

    Article  PubMed  CAS  Google Scholar 

  • von Witsch M, Baluska F, Staiger CJ and Volkmann D (1998) Profilin is associated with the plasma membrane in microspores and pollen. Eur J Cell Biol 77: 303–312

    Article  Google Scholar 

  • Wasserman S (1998) FH proteins as cytoskeletal organizers. Trends Cell Biol 8:111–115

    Article  PubMed  CAS  Google Scholar 

  • Welch MD, Rosenblatt J, Skoble J, Portnoy DA and Mitchison TJ (1998) Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation. Science 281: 105–108

    Article  PubMed  CAS  Google Scholar 

  • Yeh J and Haarer BK (1996) Profilin is required for the normal timing of actin polymerization in response to thermal stress. FEBS Lett 398: 303–307

    Article  PubMed  CAS  Google Scholar 

  • Yu L-X, Nasrallah J, Valenta R and Parthasarathy MV (1998) Molecular cloning and mRNA localization of tomato pollen profilin. Plant Mol Biol 36: 699–707

    Article  PubMed  CAS  Google Scholar 

  • Zhao K, Wang WD, Rando OJ, Xue YT, Swiderek K, Kuo A and Crabtree GR (1998) Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Cell 95: 625–636

    Article  PubMed  CAS  Google Scholar 

  • Zigmond SH (1996) Signal transduction and actin filament organization. Curr Opin Cell Biol 8: 66–73

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907-1392, USA

    Bryan C. Gibbon & Christopher J. Staiger

Authors
  1. Bryan C. Gibbon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christopher J. Staiger
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

C. J. Staiger F. Baluška D. Volkmann P. W. Barlow

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Gibbon, B.C., Staiger, C.J. (2000). Profilin. In: Staiger, C.J., Baluška, F., Volkmann, D., Barlow, P.W. (eds) Actin: A Dynamic Framework for Multiple Plant Cell Functions. Developments in Plant and Soil Sciences, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9460-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-94-015-9460-8_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5504-0

  • Online ISBN: 978-94-015-9460-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation