Abstract
The toxicity of rice cell exometabolites to blast spores was studied. The cultivars represented the host susceptibility or resistance (complete, partial or mixed). Diffusates collected from intact leaves of the susceptible cultivar, 1 or 2 days after inoculation, was found to inhibit spore germination weakly whereas this ability was significantly stronger in all resistant cultivars. Active oxygen (AO) species (H2O2 or.OH) may be involved in the toxicity since the latter was abolished by appropriate scavengers. The contribution of radical O2 to the toxicity was large in completely resistant cultivars and absent in the partially resistant ones. Cultivars with mixed type of resistance were intermediate in this respect. So, the leaf diffusate fungitoxicity was likely indicative of the resistance. Analogous antifungal responses were found in rice callus cultures, with the similar specificity to fungus strains as in intact plants from which the cultures were derived. Diffusates of spore-inoculated calli were weakly if any fungitoxic in compatible combinations but strongly toxic in incompatible ones. The reaction depended on Ca2+ ions. During the first hours after inoculation, the toxicity of callus diffusates was mediated by AO and then by other substances formed as a result of AO production. Therefore, rice cells in a callus culture retain fungitoxic responses characteristic of the varietal resistance and peculiar to intact plants.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aver’yanov, A.A. and Lapikova, V.P. (1988) Fungitoxicity determined by active forms of oxygen in excretions of rice leaves, Soviet Plant Physiology 35, 873–881.
Baker, C.J. and Orlandi, E.W. (1995) Active oxygen in plant pathogenesis, in: Annu.Rev.Phytopathol, vol. 33, Annual Reviews Inc., Palo Alto, pp. 299–321.
Correa-Victoria, F.J. and Zeigler, R.S. (1994) Pathogenic variability in Pyricularia grisea at a rice blast “hot spot” breeding site in eastern Colombia, Plant Disease 77, 1029–1035.
Hayashi, N., Ando, I., and Imbe, T. (1998) Identification of a new resistance gene to a Chinese blast fungus isolate in the Japanese rice cultivar Aichi Asahi, Phytopathology 88, 822–827.
Kiyosawa, S. (1976) Method for testing and gene analyses of blast resistance of rice varieties, Orvza 13, I32.
Lapikova, V.P., Aver’yanov, A.A., Petelina, G.C., Kolomiets, T.M., and Kovalenko, E.D. (1994) Fungitoxicity of leaf excretions as related to varietal rice resistance to blast, Ru.csiart J. of Plant Phvsiol. 41, 108–113.
Latterel, F.M., Marchetti, M.A., and Grove, B.R. (1965) Co-ordination of effort to establish an international system for race identification in Piricularia oryzae, in: R.F. Chandler (ed.), The rice blast disease. Proc. of a Symp. at the Internat. Rice Research Institute, The Johns Hopkins Press, Baltimore, pp. 257–274.
Pasechnik, T.D., Aver’yanov, A.A., Lapikova, V.P., Kovalenko, E.D., and Chernova, N.A. (1995) Fungitoxicity of rice leaf excretion due to activated oxygen: contribution to partial resistance to blast disease, Russian J. of Plant Physiol. 42, 330–337.
Pasechnik, T.D., Lapikova, V.P., and Aver’yanov, A.A. (1997) Inhibition of pre-penetration development of blast fungus during the infection of resistant rice cultivars, Eur. J. of Plant Pathol. 103, 747–750
Wojtaszek, P. (1997) Oxidative burst: an early plant response to pathogen infection, Blochent. J. 322, 681–692.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Aver’yanov, A.A., Pasechnik, T.D., Lapikova, V.P., Gaivoronskaya, L.M. (2000). Fungitoxic Responses of Rice Leaves and Calli as Related to Blast Resistance. In: Tharreau, D., Lebrun, M.H., Talbot, N.J., Notteghem, J.L. (eds) Advances in Rice Blast Research. Developments in Plant Pathology, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9430-1_11
Download citation
DOI: https://doi.org/10.1007/978-94-015-9430-1_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5436-4
Online ISBN: 978-94-015-9430-1
eBook Packages: Springer Book Archive