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Use of 14C-Labeled Alfalfa Saponins for Monitoring Their Fate in Soil

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Saponins in Food, Feedstuffs and Medicinal Plants

Part of the book series: Proceedings of the Phythochemical Society of Europe ((PPSE,volume 45))

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Abstract

Alfalfa saponins are known to exhibit allelopathic activity, reducing yields of succeeding plants following alfalfa cultivation [1–3], and decreasing growth and/or activity of soil microorganisms [4, 5]. The fate of these saponins in soil has received limited study to date. Inhibition of wheat seedling growth in soils treated with powdered alfalfa roots was less in heavy loam and loamy sand soils than in a loose or coarse sand [6]. In this work alfalfa root saponins were adsorbed from water solutions more by heavy-textured soils than by light-textured soils. Bioassay with Trichoderma viride of inhibitory saponins left in water after incubation with soil was used by these authors to estimate saponin adsorption. Bioassays, however, are an indirect method for following the fate of saponins in soil.

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References

  1. Guenzi, W.D., Kehr, W.R. and Mccalla, T.M. (1964) Water-soluble phytotoxic substances in alfalfa forage: variation with variety, cutting, year, and stage of growth. Agronomy Journal 56, 499–500.

    Article  Google Scholar 

  2. Golpen, B.P. and Webster, G.R. (1969) Selection in Medicago sativa for tolerance to alfalfa-sick soils of central Alberta. Agronomy Journal 61, 589–590.

    Article  Google Scholar 

  3. Waller, G.R., Jurzysta, M. and Thorne, R L Z (1995) Root saponins from alfalfa (Medicago sativa L.) and their allelopathic activity on weeds and wheat. Allelopathy Journal 2, 21–30.

    Google Scholar 

  4. Levy, M., Zehavi, U., Naim, M., Polachek, I. and Evron, R. (1989) Structure-biological activity relationships in alfalfa antimycotic saponins: The relative activity of medicagenic acid and synthetic derivatives thereof against plant pathogenic fungi. J. Phytopath. 125, 209–216.

    Article  CAS  Google Scholar 

  5. Oleszek, W., Price, K.R., Coloquhoun, I.J., Jurzysta, M., Ploszynski, M. and Fenwick, G. R. (1990) Isolation and identification of alfalfa (Medicago sativa L.) root saponins: Their activity in relation to a fungal bioassay. J. Agric. Food Chem. 38, 1810–1817.

    Article  CAS  Google Scholar 

  6. Oleszek, W. and Jurzysta, M. (1987) The allelopathic potential of alfalfa root medicagenic acid gylcosides and their fate in the soil environment. Plant and Soil 98, 67–80.

    Article  CAS  Google Scholar 

  7. Oleszek, W., Jurzysta, M., Price, K. R. and Fenwick, G.R. (1990) High-performance liquid chromatography of alfalfa root saponins. J. Chrom. 519, 109–116.

    Article  CAS  Google Scholar 

  8. Nowacki, E., Jurzysta, M. and Dietrych-Szostak, D. (1976) Biosynthesis of medicagenic acid in germinating alfalfa. Biochem. Physiol. Pflanze. 156, 183–186.

    Google Scholar 

  9. Fuggersberger-Heinz, R. and Franz, G. (1984) Formation of glycyrrhizinic acid in Glycyrrhiza glaba var. typica. Planta Medica 50, 409–413.

    Article  CAS  Google Scholar 

  10. Martin, J. W. (1975) Comparison of agar media for counts of viable soil bacteria. Soil Biol. Bloch. 7, 40 1402.

    Google Scholar 

  11. Hoagland, D.R. and Arnon, D.I. (1950) The water-culture method for growing plant without soil. California Agric. Expn. Stat. Circ. 347.

    Google Scholar 

  12. Schnitzer, M. (1982) Organic matter characterization, in A.L. Page, R.H. Miller and D.R. Keeney (eds), Methods of Soil Analysis, American Society of Agronomy, Madison, WI, 9, pp. 581–594.

    Google Scholar 

  13. Filonow, A.B., Vishniac, H.S., Anderson, J.A. and Janisiewicz, W.J. (1996) Biological control of Botrytis cinerea in apple by yeasts from various habitats and their putative mechanisms of antagonism. Biological Control 7, 212–220.

    Article  Google Scholar 

  14. Vance, E.D., Brookes, P.C. and Jenkinson, D.S. (1987) An extraction method for measuring soil microbial biomass C. Soil Biol. Bioch. 19, 703–707.

    Article  CAS  Google Scholar 

  15. Wyman-Simpson, C.L., Waller, G.R., Jurzysta, M., Mcpherson, J.K. and Young, C.C. (1991) Biological activity and chemical isolation of root saponins of six cultivars of alfalfa (Medicago sativa L.). Plant and Soil 135, 83–94.

    Article  CAS  Google Scholar 

  16. Peri, I. Mor, U., Heftmann, E., Bondi, A. and Tencer, Y. (1979) Biosynthesis of triterpenoid sapogenols in soybean and alfalfa seedlings. Phytochemistry 18, 1671–1674.

    Article  CAS  Google Scholar 

  17. Martin, J.K. (1975)14C-labelled material leached from the rhizosphere of plants supplied continuously with 14CO2. Soil Biol. Bioch. 7, 395–399.

    Google Scholar 

  18. Stevenson, F.J. (1994) Humus chemistry: genesis, composition, reactions. John Wiley and Sons, New York. pp. 460.

    Google Scholar 

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Okumura, M., Filonow, A.B., Waller, G.R. (2000). Use of 14C-Labeled Alfalfa Saponins for Monitoring Their Fate in Soil. In: Oleszek, W., Marston, A. (eds) Saponins in Food, Feedstuffs and Medicinal Plants. Proceedings of the Phythochemical Society of Europe, vol 45. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9339-7_19

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  • DOI: https://doi.org/10.1007/978-94-015-9339-7_19

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5341-1

  • Online ISBN: 978-94-015-9339-7

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