Abstract
Mass spectrometry has long been viewed to possess the ability to readily contribute to the rapid, sensitive and accurate characterization of biomolecules. However, it has been only within the last decade, with the advent of electrospray ionization (ESI) [1] and matrix-assisted laser desorption/ionization (MALDI) [2] mass spectrometries, that routine biomolecular analyses at physiological levels have become a reality. Indeed, these techniques have opened the door to the regular practice of biological mass spectrometry (at the bench level), and in doing so, have paved the way for a number of mass spectrometric-based methods directed at the intricate investigation of biomolecular structure and function. Generally, these methods revolve around the combination of other analytical techniques or methodologies (e.g. chromatography, electrophoresis, enzymatic/chemical treatment of analyte) with mass spectrometry. In order to achieve the highest levels of analytical performance, the integration of techniques must be performed in manners which minimize sample losses, maintain a desired rapidity, and preserve a high level of accuracy.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Fenn, J. B., Mann, M., Meng, C. K. Wong, S. F. and Whitehouse, C. M. (1989) Electrospray Ionization for Mass Spectrometry of Large Biomolecules, Science 246, 64–71.
Karas, M. and Hillenkamp, F. (1988) Laser Desorption/Ionization of Proteins with Molecular Mass Exceeding 10,000 Da, Anal. Chem. 60, 2288–2301.
Hutchens, T. W. and Yip, T. T. (1993) New Desorption Strategies for the Mass Spectrometric Analysis of Macromolecules, Rapid Commun. Mass Spectrom. 7, 576–580.
Zhao, Y., and Chait, B. T. (1994) Protein Epitope Mapping by Mass Spectrometry. Anal. Chem. 66, 37233726.
Zhao, Y., Muir, T. W., Kent, S. B. H., Tischer, E., Scardina, J. M., and Chait, B. T. (1996) Mapping Protein-Protein Interactions by Affinity-Directed Mass Spectrometry, Proc. Natl. Acad. Sci. USA 93, 40204024.
Brockman, A. H., and Orlando, R. (1995) Probe-Immobilized Affinity Chromatography/ Mass Spectrometry, Anal. Chem. 67, 4581–4585.
Nelson, R. W., Krone, J. R., Bieber, A. L., and Williams, P. (1995) Mass Spectrometric Immunoassay, Anal. Chem. 67, 1153–1158.
Krone, J. R., Nelson, R. W. and Williams P. (1996) Mass Spectrometric Immunoassay, Proceedings of Ultasensitive Biochemical Diagnostics, SPIE 2680, 415–421.
Krone, J. R. (1996) Mass Spectrometric Methods for Application in Immunoassay and Biomolecular Characterization, Ph.D. Thesis.
Nelson, R.W., McLean, M.A., and Hutchens, T.W. (1994) Quantitative Determination of Proteins in Solution by Matrix-Assisted Laser Desorption/Ionization Time-of-Right Mass Spectrometry, Anal. Chem. 66, 1408–1415.
Muddiman, D. C., Gusev, A. I., and Hercules, D. M. (1996) Application of Secondary Ion and Matrix-Assisted Laser Desorption/Ionization Time-of-Right Mass Spectrometry for the Quantitative Analysis of Biological Molecules, Mass Spectrom. Rev. In press.
Voet, D. and Voet, J. G. (1995) Biochemistry, John Wiley and Sons, Inc., New York, NY.
Hillenkamp, F. (1996) in these proceedings.
McGeer, P. L., Akiyama, H., Itagaki, S. and McGeer, E.,G. (1989) Activation of the Classical Complement Pathway in Brain Tissue of Alzheimer Patients, Neurosci. Lett. 107, 341–346.
Li, Y., McIver, R. T. and Hunter, R. L. (1994) High-Accuracy Molecular Mass Determination for Peptides and Proteins by Fourier Transform Mass Spectrometry, Anal. Chem. 66, 2077–83.
Figeys, D., Vanoostveen, I., Ducret, A. and Aebersold R. (1996) Protein Identification by Capillary Zone Electrophoresis/Microelectrospray Ionization-Tandem Mass Spectrometry at the Subfemtomole Level, Anal. Chem. 68, 1822–1828.
Henzel, W. J., Billeci, T. M., Stults, J. T., Wong, S. C., Grimley, C. and Watanabe, C. (1993) Identifying Proteins from Two-Dimensional Gels by Molecular Mass Searching of Peptide Fragments in Protein Sequence Databases, Proc. Natl. Acad. Sci. USA 90, 5011–5015.
Zaia J., Annan R. S. and Biemann K. (1992) The Correct Molecular Weight of Myoglobin, A Common Calibrant for Mass Spectrometry, Rapid Comm. in Mass Spectrom. 6 32–36.
Dogruel, D., Williams, P. and Nelson, R. W. (1995) Rapid Tryptic Mapping using Enzymatically-Active Mass Spectrometer Probe Tips, Anal. Chem. 67, 4343–4348.
Nelson, R. W., Dogruel, D., Krone, J.R. and Williams, P. (1995) Peptide Characterization Using Bioreactive Mass Spectrometer Probe Tips, Rapid Comm. Mass Spectrom. 9, 1380–1385.
Dogruel, D., Nelson, R. W., Krone, J. R., Lewis, J. K. and Williams, P. (1995) Rapid Protein Characterization Using Bioreactive Mass Spectrometer Probe Tips, Proceedings of Ultasensitive Biochemical Diagnostics, SPIE 2680, 390–396.
Nelson, R. W., Krone, J. R., Dogrel, D., Tubbs, K. A., Granzow, R. and Jansson, O. (1997) Interfacing Biomolecular Interaction Analysis with Mass Spectrometry and the Use of Bioreactive Mass Spectrometer Probe Tips in Protein Characterization, in D. Marshak (ed.), Techniques in Protein Chemistry VIII, Academic Press, San Diego. In press.
Krone, J. R., Nelson, R. W., Dogruel, D., Williams, P. and Granzow, R. (1996) Interfacing Mass Spectrometric Immunoassays with BIA, BIAjournal 3, 16–17.
Krone, J. R., Nelson, R. W., Dogruel, D., Granzow, R. and Williams, P. (1997) BIA/MS: Interfacing Biomolecular Interaction Analysis with Mass Spectrometry, Anal. Biochem. 244, 124–132.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Nelson, R.W., Krone, J.R., Tubbs, K.A., Dogruel, D. (1998). Mass Spectrometric Methods for Biomolecular Characterization. In: Ens, W., Standing, K.G., Chernushevich, I.V. (eds) New Methods for the Study of Biomolecular Complexes. NATO ASI Series, vol 510. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9046-4_19
Download citation
DOI: https://doi.org/10.1007/978-94-015-9046-4_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5017-5
Online ISBN: 978-94-015-9046-4
eBook Packages: Springer Book Archive