Abstract
The ability to use peptide synthesis to produce small synthetic proteins containing approximately 100 amino acids has been demonstrated in the past with the syntheses of Ribonuclease-A, Interleukin-3, HIV-1 Protease, and Ubiquitin [1–4]. However these syntheses result in poor yields of the desired product which are difficult to purify because of the heterogeneity of the crude protein [5].
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References
Gutte, B.; Merrifield, R.B., (1971), The Synthesis of Ribonuclease A., J.Biol.Chem., 246, 1922–1941.
Clarke-Lewis, I.; Abersold, R.; Ziltner, H.; Schrader, J.W.; Hood, L.E.; Kent, S.B.H., (1988), Automated Chemical Synthesis of a Protein Growth Factor for Hemopoietic Cells, Interleukin 3., Science, 231, 134–139.
Schneider, J.; Kent, S.B.H., (1988), Enzymatic Activity of a synthetic 99 Residue Protein Corresponding to the Putative HIV-1 Protease, Cell, 54, 3128–3134.
Ramage, R.; Green, J.; Ojunjobi, O.M., (1987), Solid Phase Synthesis of Ubiquitin, Tetrahedron Lett., 30, 2149–2153.
Kent, S.B.H.; Alewood, D.; Alewood, P.; Baca, M.; Jones, A.; Schnolzer, M., (1992)Total chemical Synthesis of Proteins: Evolution of Solid Phase Synthetic Methods Illustrated by Total Chemical Synthesis of the HIV-1 Protease.; in Solid Phase Synthesis: Peptides, Polypeptides and Polynucleotides, Epton. R. Ed.; Intercept Limited: Andover, 1992, pp 1–22.
Schnolzer, M.; Kent, S.B.H., (1992), Constructing Proteins by Dovetailing Unprotected Synthetic Peptides: Backbone-Engineered HIV Protease., Science, 256, 221–225.
Dawson, P.E.; Muir, T.W.; Clark-Lewis, I.; Kent, S.B.H., (1994), Synthesis of Proteins by Native Chemical Ligation, Science, 266, 776–779.
Williams, M.J.; Muir, T.W.; Ginsberg, M.H.; Kent, S.B.H., (1994), Total Chemical Synthesis of a Folded Beta-Sandwich Protein Domain–An Analog of the Tenth Fibronectin Type 3 Module, JAmer. Chem.Soc., 116, 10797–10798.
Muir, T.W.; Williams, M.J.; Ginsberg, M.H.; Kent, S.B.H., (1994), Design and Chemical Synthesis of a Neoprotein Structural Model for the Cytoplasmic Domain of a Multisubunit Cell-Surface Receptor- Integrin Alpha(IIb)Beta(3) (Platelet GPII3-IIIa), Biochemistry, 33, 7701–7708.
Englebretsen, D.R.; Gamham, B.G.; Bergman, D.A.; Alewood, P.F., (1995), A Novel Thioether Linker–Chemical Synthesis of a HIV-1 Protease Analogue by Thioether Ligation, Tetrahedron Lett., 36, 8871–8874.
Hunt, J.F.; Weaver, A.J.; Landry, S.J.; Gierasch, L.; Deisenhofer, J., (1996), The Crystal Structure of the GroES Co-Chaperonin At 2.8-Angstrom Resolution, Nature, 379, 37–45.
Mande, S.C.; Mehra, V.; Bloom, B.R., (1996), Structure of the Heat Shock Protein Chaperonin-10 of Mycobacterium Leprae., Science, 271, 203–207.
Morton, H.; Cavanagh, A.C.; Athanasas-Platsis, S.; Quin, K.A.; Rolfe, B.E., (1992), Early Pregnancy Factor has Immunosupressive and Growth Factor Properties., Reproduction Fertility and Development., 4, 411–422.
Cavanagh, A.C., Morton, H., (1994), The Purification of Early-Pregnancy Factor to Homogeneity from Human Platelets and Identification as Chaperonin 10, Euro.J. Biochem. 222, 551–560.
Summer, K.M., Murphy, R.M., Webb, G.G., Peters, G.B., Morton, H., Cassady, A.I. Cavanagh, A.C., (1996), Biomedical and Molecular Medicine, 58 52–58.
Hartl, F. U.; Martin, J., (1995), Molecular Chaperones in Cellular Protein Folding., Current Opinion in Structural Biology., 5, 92–102.
Saibil, H., (1996), The Lid that Shapes the Pot: Structure and Function of the Chaperonin GroES, Structure, 4, 1–4.
Schnolzer, M.; Alewood, P.; Alewood, D.; Jones, A.; Kent, S.B.H., (1992), In situ Neutralisation in Boc-Chemistry Solid Phase Peptide Synthesis., Int.J. Pep. Prot. Res., 40, 180–193.
Verentchikov, A.N., Ens, W., Standing, K.G., (1994), Reflecting Time-of-Flight Mass Spectrometer with an Electrospray Ion Source and Orthogonal Extraction, Anal. Chem. 66, 126–133.
Tang, X.; Brewer, C.F.; Salsa, S.; Chernushevich, I.; Ens, W.; Standing, K.G., (1994), Investigation of Protein-Protein Noncovalent Interactions in Soyabean Agglutinin by Electrospray Ionization Time-ofFlight Mass Spectrometry, Rapid Communications in Mass Spectrometry, 8, 750–754.
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© 1998 Springer Science+Business Media Dordrecht
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Love, S.G. et al. (1998). Observation of Protein Heptamer Formation for Chemically Ligated Early Pregnancy Factor. In: Ens, W., Standing, K.G., Chernushevich, I.V. (eds) New Methods for the Study of Biomolecular Complexes. NATO ASI Series, vol 510. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9046-4_14
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DOI: https://doi.org/10.1007/978-94-015-9046-4_14
Publisher Name: Springer, Dordrecht
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