Abstract
Electrospray ionisation (ESI) is a “soft” ionisation method which produces ions from solution by nebulisation in an electric field. It allows the direct analysis of samples in solution with a mixture of usual solvents (H2O, CH3OH, CH3CN), and has rapidly become an essential method for the analysis of biological compounds (peptides, proteins, organometalics, organic salts).
The widespread use of ESI would benefit from a database of ESI-spectra. This requires the harmonisation of methods for recording spectra and presupposes an understanding of phenomena underlying the method. Because the internal energy of the ions governs their fragmentation and therefore the mass spectral pattern, we developed a method for its determination. In this study, the fragmentation of a set of probe molecules (benzylpyridinium salts) was studied to determine the internal energy distribution of ions emitted by the electrospray source under various conditions (the voltage and the collision gas). The correlation of the internal energy with the experimental conditions will allow calibration of individual working conditions and therefore comparison of results obtained on different spectrometers.
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© 1998 Springer Science+Business Media Dordrecht
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Moonen, F., Collette, C., De Pauw, E. (1998). On the Determination of the Ion Internal Energy in an Electrospray Source. In: Ens, W., Standing, K.G., Chernushevich, I.V. (eds) New Methods for the Study of Biomolecular Complexes. NATO ASI Series, vol 510. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-9046-4_13
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DOI: https://doi.org/10.1007/978-94-015-9046-4_13
Publisher Name: Springer, Dordrecht
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