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Enzyme Sensors for the Detection of Pesticide Families

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Book cover Biosensors for Direct Monitoring of Environmental Pollutants in Field

Part of the book series: NATO ASI Series ((ASEN2,volume 38))

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Abstract

Biosensors for the detection of low levels of pesticides were prepared by entrapment of different enzymes in polyvinyl-alcohol bearing styryl pyridinium groups (PVA-SbQ). The inhibition of aldehyde dehydrogenase (EC 1.2.1.5), acetolactate synthase (EC 4.1.3.18) and acetyl cholinesterase (EC 3.1.1.7) allows respectively the detection of dithiocarbamate fungicides (10−8 M), sulfonylurea herbicides (10−6 M) and organophosphorus insecticides (10−7 M). The detection is amperometrically carried out.

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References

  1. Rogers, K.R. (1995) Biosensors for environmental applications, Biosensors & Bioelectronics 10, 533–541.

    Article  CAS  Google Scholar 

  2. Ortega, F., Bastide, J., and Hawkes, T.R. (1996) Comparison between thifensulfuron methyl-induced inactivation of barley acetohydroxyacid synthase and Escherichia coli acetohydroxyacid synthase isozyme II, Pestic. Biochem. Physiol. 56, 231–242.

    CAS  Google Scholar 

  3. Rouillon, R., Mestres, J.-J., and Marty, J.-L. (1995) Entrapment of chloroplasts and thylakoids in polyvinyl alcohol polymers. Optimization of membrane preparation and storage conditions, Anal. Chim. Acta 311, 437–442.

    Article  CAS  Google Scholar 

  4. Iimes, J.R.M., Ulland, B.M., Valerio, M.G. (1969) Bioassay of pesticides and industrial chemicals for tumorigenicity in mice, J. Nath. Cancer Inst. 42, 1101–1114.

    Google Scholar 

  5. Seiler. J.Y.(I974) Ethylenethiourea (ETU)_ a carcinogenic and mutagenic metabolite of ethylenebisdithiocarbamate,.Nutat. Res. 26, I89–191.

    Google Scholar 

  6. Graham, S.L., Davis, K.J., Hansen W.H., and Graham, C H (1975) Effects of prolonged ethylenethiourea ingestion on the thyroid of the rat, Food Cosmetol. Toxicol. 13, 493–499.

    Article  CAS  Google Scholar 

  7. Teramoto, S., Moriya, M., Kato, K., Tezuka, H., Nakamura, S., Shingu, A. and Shirasu, Y. (1977) Mutagenicity testing on ethylenethiourea, Mutat. Res. 56, 121–129.

    Article  CAS  Google Scholar 

  8. Teramoto, S., Saito, R. and Shirasu, Y. (1980) Teratogenic effects of combined administration of ethylenethiourea and nitrate in mice, Teratology 21, 71–78.

    Article  CAS  Google Scholar 

  9. Vincent, G. (1991) Organic pollutants in the aquatic environment, G. Angelotti and A. Bjorseth, Eds.,Kluwer Academic Publishers, Dordrecht, pp. 285–291.

    Google Scholar 

  10. Fielding, M., Barcelò, D., Helweg, A., Galassi, S., Torstensson, L., van Zoonen, P., Wolter, R., and Angeletti, G. (1992) Pesticides in Ground and Drinking Water (Water Pollution Research Report, 27),Commission of the European Communities, Brussels, pp. 1–136.

    Google Scholar 

  11. Horwitz, W. (Ed.). (1970)Association of Official Analytical Chemists, Official Methods ofAnalysis,11th ed, Washington, D.C, p. 118.

    Google Scholar 

  12. Keppel, G.E. (1971) Collaborative study of the determination of dithiocarbamate residues by a modified carbon disulfide evolution method, J. Assoc. Off Anal. Chem., 54, 528–532.

    CAS  Google Scholar 

  13. Jongen, M.J.M., Ravensberg, J.C., Engel, R., and Leenheers, L.H. (1991) Gas-liquid and liquid chromatographic determination of maneb for the assessment of occupational exposure in the production of ornamentals, J. Chrom. Sc., 29, 292–297.

    CAS  Google Scholar 

  14. Lehotay, J., and Kisovà, D. (1993) HPLC study of mancozeb degradation on leaves, J. Liq. Chromatogr. 16, 1015–1022.

    Article  CAS  Google Scholar 

  15. Hakan Gustafsson, K., and Thompson, R.A. (1981) High-pressure liquid chromatographic determination of fungicidal dithiocarbamates, J. Agric. Food Chem. 29, 729–732.

    Article  CAS  Google Scholar 

  16. Woodrow, J.E., Seiber, J.N., and Fitzell, D. (1995) Analytical method for the dithiocarbamate fungicides ziram and mancozeb in air: preliminary field results, J. Agric. Food Chem. 43, 1524–1529.

    Article  CAS  Google Scholar 

  17. Wiegand-Rosinus, M., Obst, U., Haberer, K., and Wild, A. (1992) Enzymes in vitro as indicators for pesticides: an examination, Environ. Toxicol. Wat. Quality 7, 313–321.

    Article  CAS  Google Scholar 

  18. Beyer, E.M., Duffy, M.J., Hay, J.V, and Schlueter, D.D. (1987) Herbicides: Chemistry, degradation, and mode of action, P.C. Kearney and D.D. Kaufman, Eds. M. Dekker, New York, pp 118–189.

    Google Scholar 

  19. LaRossa, R.A., Schloss, J.V. (1984) The sulfonylurea harbicide sulfometuron methyl is an extremely potent and selective inhibitor of acetolactate synthse in Salmonella typhimurium, J. Biol. Chem. 259, 8753–8757.

    CAS  Google Scholar 

  20. Abell, L.M., and Schloss, J.V. (1991) Oxygenase side reactions of acetolactate synthase and other carbanionforming enzymes, Biochem. 30, 7883–7887.

    Article  CAS  Google Scholar 

  21. Muhitch, M.J., Shaner, D.L., Stidham, M.A. (1987) Imidazolinones and acetohydroxyacid synthase from higher plants, Plant Physiol. 83, 451–456.

    Article  CAS  Google Scholar 

  22. Schloss, J.V. (1990) Acetolactate synthase, mechanism of action and its herbicide binding site, Pestic. Sci. 29, 283–292.

    Article  CAS  Google Scholar 

  23. Eto, M. (1977) Organophosphorus Pesticides: Organic and Biological Chemistry, CRC Press, Cleveland, OH.

    Google Scholar 

  24. Minelli, E.V., Cabras, P., Angioni, A., Garau, V.L., Melis, M., Pirisi, F.M., Cabitza, F., and Cubeddu, M. (1996) Persistence and metabolism of fenthion in orange fruit, J. Agric. Food Chem. 44, 935–939.

    Article  Google Scholar 

  25. Lacorte, S., and Barcelò, D. (1995) Determination of organophosphorus pesticides and their transformation products in river waters by automated on-line solid-phase extraction followed by thermospray liquid chromatography-mass spectrometry, J. Chromatogr. A712, 103–112.

    Article  CAS  Google Scholar 

  26. Lentza-Rizos, Ch., Avramides, E.J., and Roberts, R.A. (1994) Persistence of fenthion residues in olive oil, Pestic. Sci., 40, 63–69.

    Article  CAS  Google Scholar 

  27. Espinosa-Mansilla, A., Salinas, F., and Zamoro, A. (1994) Kinetic study of degradation of chlorpyrifos by using a stopped-flow FIA system. Semiautomatic determination in commercial formulations, Talanta 41, 651–657.

    Article  CAS  Google Scholar 

  28. Lacorte, S., and Barcelò, D. (1994a) Validation of an automated precolumn exchange system (Prospekt) coupled to liquid chromatography with diode array detection. Application to the determination of pesticides in natural waters, Anal. Chim. Acta 296, 223–234.

    Article  CAS  Google Scholar 

  29. Lacorte, S. and Barcelò, D. (1994b) Rapid degradation of fenitrothion in estuarine waters, Environ. Sci. Technol. 28, 1159–1163.

    Article  CAS  Google Scholar 

  30. Lacorte, S., Ehresmann, N., and Barcelò, D. (1996) Persistence of temephos and its transformation products in rice crop field waters, Environ. Sci. Technol. 30, 917–923.

    Article  CAS  Google Scholar 

  31. Bellar, T.A., and Budde, W.L. (1988) Determination of nonvolatile organic compounds in aqueous environmental samples using liquid chromatography/mass spectrometry, Anal. Chem. 60, 2076–2083.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Centre de Phytopharmacie, URA CNRS N° 461, 52 avenue de Villeneuve, 66860, Perpignan Cedex, France

    J.-L. Marty, T. Noguer, A. Seki & G. Jeanty

Authors
  1. J.-L. Marty
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  2. T. Noguer
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  3. A. Seki
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  4. G. Jeanty
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Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, 15771, Athens, Greece

    Dimitrios P. Nikolelis

  2. Erindale Campus, Chemistry Department, University of Toronto, 3359 Mississauga Rd. N., L5L 1C6, Mississauga, Ontario, Canada

    Ulrich J. Krull

  3. Department of Chemistry and Biochemistry, New Mexico State University, Las Cruses, New Mexico, USA

    Joseph Wang

  4. Institute of Analytical Chemistry, University of Firenze, Firenze, Italy

    Marco Mascini

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© 1998 Springer Science+Business Media Dordrecht

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Marty, JL., Noguer, T., Seki, A., Jeanty, G. (1998). Enzyme Sensors for the Detection of Pesticide Families. In: Nikolelis, D.P., Krull, U.J., Wang, J., Mascini, M. (eds) Biosensors for Direct Monitoring of Environmental Pollutants in Field. NATO ASI Series, vol 38. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8973-4_16

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  • DOI: https://doi.org/10.1007/978-94-015-8973-4_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4959-9

  • Online ISBN: 978-94-015-8973-4

  • eBook Packages: Springer Book Archive

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