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Biosensors for Direct Monitoring of Environmental Pollutants in Field pp 155–162Cite as

Detection of Diphenylurea Derivatives with Biospecific Interaction Analysis (BIA): Kinetic Investigations

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Part of the book series: NATO ASI Series ((ASEN2,volume 38))

Abstract

Monoclonal antibodies against a derivative of diphenylurea and the derived Fab fragments were investigated using a BIACORE® system with respect to binding kinetics, thermodynamics and assay characteristics. The chloroacetyl group of the hapten II used for immunization and immobilization was involved in the antibody binding. The binding of Fab to immobilized hapten was enthalpy driven below 37°C. Ethanol led to decreased affinities, but shifted the crossreactivity in favour of hapten II.

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References

  1. Wolter, R. (1995) Pflanzenschutzmittelfunde im Wasser, Forum Gewässerschutz und Pflanzenschutz, Industrieverband Agrar, Bonn and Umweltbundesamt Berlin - Information

    Google Scholar 

  2. Price, D.R.H. (1991) Pesticide residues in water supplies, Brighton Crop Protection Conf.-Weeds, 1279–1284

    Google Scholar 

  3. Karu, A.E., Goodrow, M.H., Schmidt, D.J., Hammock B.D. and Bigelow, M.W. (1994) Synthesis of haptens and derivation of monoclonal antibodies for immunoassay of the phenylurea herbicide diuron, J. Agric. Food Chem. 42, 301–309

    Article  CAS  Google Scholar 

  4. Katmeh, M.F., Frost, G., Aherne, G.W. and Stevenson, D. (1994) Development of an enzyme-linked immunosorbent assay for isoproturon in water, Analyst 119, 431–435

    Article  CAS  Google Scholar 

  5. Katmeh, M.F., Aherne, G.W. and Stevenson, D. (1996) Development and evaluation of a chemiluminescent immunoassay for chlortoluron using a camera luminometer, Analyst 121, 329–332

    Article  CAS  Google Scholar 

  6. Krämer, P. and Schmid, R.D. (1992) Biosensors for monitoring pesticides in water, in W. Göpel, J. Hesse and J.N. Zemel (eds.) Sensors - A Comprehensive Survey, vol. 3, VCH, Weinheim, pp. 10131021

    Google Scholar 

  7. Neri, D., Montigiani, S. and Kirkham, P.M. (1996) Biophysical methods for the determination of antibody-antigen affinities, Trends Biotechnol. 14, 465–470

    Article  CAS  Google Scholar 

  8. Lindner, W. and Robey, F.A. (1987) Automated synthesis and use of N-chloroacetyl-modified peptides for the preparation of synthetic peptide polymers and peptide-protein immunogens, Int. J. Peptide Protein Res. 30, 794–800

    Article  CAS  Google Scholar 

  9. Karlsson, R., Fägerstam L., Nilshans, H. and Persson, B. (1993) Analysis of active antibody concentration. Separation of affinity and concentration parameters, J. Immunol. Meth. 166, 75–84

    Article  CAS  Google Scholar 

  10. Friguet, B., Chaffotte, A.F., Djavadi-Ohaniance, L. and Goldberg, M.E. (1985) Measurements of the true affinity constant in solution of antigen-antibody-complexes by enzyme-linked Immunosorbent assay, J. Immunol. Meth. 77, 305–319

    Article  CAS  Google Scholar 

  11. R. Karlsson (1994) Real-time competitive kinetic analysis of interactions between low-molecularweight ligands in solution and surface-immobilized receptors, Anal. Biochem. 221, 142–151

    Article  CAS  Google Scholar 

  12. Zidovetzki, R., Blatt, Y., Schepers, G. and Pecht, I. (1988) Thermodynamics of oligosaccharides binding to a dextran-specific monoclonal IgM, Mol. Immunol. 25, 379–383

    Article  CAS  Google Scholar 

  13. Zeder-Lutz, G., Zuber, E., Witz, J. and Van Regenmortel, M.H.V. (1997) Thermodynamic analysis of antigen-antibody binding using biosensor measurements at different temperatures, Anal. Biochem. 246, 123–132

    Article  CAS  Google Scholar 

  14. Stöcklein, W.F.M., Scheuer, F.W. and Abuknesha, R (1995) Effects of organic solvents on semicontinuous immunochemical detection of coumarin derivatives, Sensors Actuators B 24–25, 80–84

    Google Scholar 

  15. De Lauzon, S., Rajkowski, K.M. and Cittanova, N. (1994) Investigation of a 17ß-estradiol-monoclonal antiestradiol antibody binding mechanism using dilute solutions of organic solvents, J. Steroid Biochem. Molec. Biol. 48, 225–233

    Article  Google Scholar 

  16. Stöcklein, W.F.M., Warsinke, A. and Scheller, F.W. (1997) Organic solvent modified enzyme linked immunoassay for the detection of triazine herbicides, in D.S. Aga and E.M. Thurman (eds.) Immunochemical technology for environmental applications, ACS Symp. Ser. Vol. 657, ACS, W ashington,pp. 373–381.

    Chapter  Google Scholar 

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Authors and Affiliations

  1. Analytische Biochemie c/o Max-Delbrück-Centrum für Molekulare Medizin (MDC), Universität Potsdam, Robert-Rössle-Strasse 10, 13122, Berlin, Germany

    W. F. M. Stöcklein, A. Warsinke & F. W. Scheller

  2. Inst. f Medizinische Immunologie, c/o MDC, Humboldt Universität, Germany

    B. Micheel

  3. Inst. f Biochemie, Humboldt Universität, Charité Berlin, Germany

    W. Höhne

  4. Inst. f Org. Chemie und Strukturanalytik, Universität Potsdam, Potsdam, Germany

    J. Woller & G. Kempter

Authors
  1. W. F. M. Stöcklein
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  2. A. Warsinke
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  3. B. Micheel
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  4. W. Höhne
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  5. J. Woller
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  6. G. Kempter
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  7. F. W. Scheller
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Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, 15771, Athens, Greece

    Dimitrios P. Nikolelis

  2. Erindale Campus, Chemistry Department, University of Toronto, 3359 Mississauga Rd. N., L5L 1C6, Mississauga, Ontario, Canada

    Ulrich J. Krull

  3. Department of Chemistry and Biochemistry, New Mexico State University, Las Cruses, New Mexico, USA

    Joseph Wang

  4. Institute of Analytical Chemistry, University of Firenze, Firenze, Italy

    Marco Mascini

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© 1998 Springer Science+Business Media Dordrecht

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Stöcklein, W.F.M. et al. (1998). Detection of Diphenylurea Derivatives with Biospecific Interaction Analysis (BIA): Kinetic Investigations. In: Nikolelis, D.P., Krull, U.J., Wang, J., Mascini, M. (eds) Biosensors for Direct Monitoring of Environmental Pollutants in Field. NATO ASI Series, vol 38. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8973-4_15

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  • DOI: https://doi.org/10.1007/978-94-015-8973-4_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4959-9

  • Online ISBN: 978-94-015-8973-4

  • eBook Packages: Springer Book Archive

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