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Synthesis and Storage of Fatty Acids

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Cellular and Molecular Biology of Plant Seed Development

Part of the book series: Advances in Cellular and Molecular Biology of Plants ((CMBP,volume 4))

Abstract

Information about the enzymology and molecular genetics of lipid synthesis in oilseeds is accumulating rapidly in part because of the commercial possibilités that can be realized through the production of modified vegetable oils. Important breakthroughs in the production of transgenic oilseed crops have already been achieved. However, many of the more complex alterations that might be possible by genetic engineering will require a better understanding of both the regulation of biochemical pathways involved and the detailed cell biology of the relationship between the plastids, endoplasmic reticulum, and oil bodies (as well as other cellular compartments). Much of this required information will become available because of the tools, antibodies, and transgenic plants, for example, resulting from newly isolated gene sequences.

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Abbreviations

ACCase:

acetyl-CoA carboxylase

ACP:

acyl carrier protein

CPT:

cholinephosphotransferase (CDP-choline:diacylglycerol cholinephosphotransferase)

DAG:

diacylglycerol

DAGAT:

diacylglycerol acyltransferase (acyl-CoA:diacylglycerol acyltransferase)

DHAP:

dihydroxyacetone phosphate

FAS:

fatty acid synthase

F6P:

fructose-6-phosphate

F1,6P:

fructose-1,6-bisphosphate

GAP:

glyceraldehyde-3-phosphate

G1P:

glucose-1-phosphate

G6P:

glucose-6-phosphate

LPA:

lyso phosphatidic acid

LPAAT:

lyso-phosphatidic acid acyltransferase (acyl-CoA:sn-1-acyl-glycerol-3-phosphate acyltransferase)

PA:

phosphatidic acid

PC:

phosphatidylcholine

PEP:

phosphoenopyruvate

PGA:

phosphoglyceric acid

6PG:

6-phosphogluconate

Ru5P:

ribulose-5-phosphate

TAG:

triacylglycerol

UDPG:

UDP-glucose

X:Y:

a fatty acyl group containing X carbon atoms and Y double bonds (cis unless specified). Double bond positions are indicated relative to the methyl carbon of the fatty acid chain (e.g., w6) or the carboxyl end of the chain (e.g., Δ12).

References

  • Agrawal, P.K., and Canvin, D.T. (1971) The pentose phosphate pathway in relation to fat synthesis in the developing castor oil seed. Plant Physiol. 47: 672–675.

    Article  PubMed  CAS  Google Scholar 

  • Alban, C., Baldet, P., and Douce, R. (1994) Localization and characterization of two structurally different forms of acetyl-CoA carboxylase in young pea leaves, of which one is sensitive to aryloxyphenoxypropionate herbicides. Biochem. J. 300: 557–565.

    PubMed  CAS  Google Scholar 

  • Appelqvist, L.A. (1975) Biochemical and structural aspects of storage and membrane lipids in developing oil seeds. In: Galliard, T., and Mercier, E.I. (eds) Recent Advances in the Chemistry and Biochemistry of Plant Lipids, pp. 247–286, Academic Press, New York.

    Google Scholar 

  • Appleby, R.S., Gurr, M.I., and Nichols, B.W. (1974) Studies on seed-oil triglycerides. Factors controlling the biosynthesis of fatty acids and acyl lipids in subcellular organelles of maturing Crambe abyssinica seeds. Eur. J. Biochem. 48: 209–216.

    Article  PubMed  CAS  Google Scholar 

  • Arondel, V., Lemieux, B., Hwang, I., Gibson, S., Goodman, H.M., and Somerville, C.R. (1992) Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science 258: 1353–1355.

    Article  PubMed  CAS  Google Scholar 

  • Bafor, M., Smith, M.A., Jonsson, L., Stobart, K., and Stymne, S. (1991) Ricinoleic acid biosynthesis and triacylglycerol assembly in microsomal preparations from developing castor bean (Ricinus communis) endosperm. Biochem. J. 280: 507–514.

    PubMed  CAS  Google Scholar 

  • Bafor, M., Smith, M.A., Jonsson, L., Stobart, K., and Stymne, S. (1993) Biosynthesis of ver-noleate (cis-12-epoxyoctadeca-cis-9-enoate) in microsomal preparations from developing endosperm of Euphorbia lagascae. Arch. Biochem. Biophys. 303: 145–151.

    Article  PubMed  CAS  Google Scholar 

  • Beevers, H. (1961) Respiratory Metabolism in Plants. Row, Peterson and Company, White Plains, New York, 234 pp.

    Google Scholar 

  • Bergfeld, R., Hong, Y.N., Kuhnl, Y., and Schopfer, P. (1978) Formation of oleosomes (storage lipid bodies) during embryogenesis and their breakdown during seedling development in cotyledons of Sinapsis alba L. Planta 143: 297–307.

    Article  CAS  Google Scholar 

  • Boddupalli, S.S., Stark, D.M., Barry, G.F., and Kishore, G.M. (1995) Effect of overexpressing ADPGlc pyrophosphorylase on the oil biosynthesis in canola. Abstract. National Plant Lipid Cooperative Symposium. South Lake Tahoe, CA, June 1–4, 1995.

    Google Scholar 

  • Browse, J. and Somerville, C. (1995) Glycerolipids. In: Meyerowitz, E. and Somerville, C. (eds) Arabidopsis. pp. 881–912, Cold Spring Harbor Press, New York.

    Google Scholar 

  • Browse, J., and Slack, C.R. (1981) Catalase stimulates linoleate desaturase activity in microsomes from developing linseed cotyledons. FEBS Lett. 131:111–114.

    Article  CAS  Google Scholar 

  • Browse, J., and Somerville, C. (1991) Glycerolipid synthesis: Biochemistry and regulation. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 467–506.

    Article  CAS  Google Scholar 

  • Browse, J., McConn, M., James, D., and Miquel, M. (1993) Mutants of Arabidopsis deficient in the synthesis of a-linolenate. Biochemical and genetic characterization of the endoplasmic reticulum linoleoyl desaturase. J. Biol. Chem. 268: 16345–16351.

    PubMed  CAS  Google Scholar 

  • Cahoon, E.B., and Ohlrogge, J.B. (1994a) Metabolic evidence for the involvement of a Δ4-palmitoyl-acyl carrier protein desaturase in petroselinic acid synthesis in coriander endosperm and transgenic tobacco cells. Plant Physiol. 104: 827–837.

    PubMed  CAS  Google Scholar 

  • Cahoon, E.B., and Ohlrogge, J.B. (1994b) Apparent role of phosphatidylcholine in the metabolism of petroselinic acid in developing umbelliferea endosperm. Plant Physiol. 104: 845–855.

    PubMed  CAS  Google Scholar 

  • Cahoon, E.B., and Shanklin, J. (1995) Engineering of the chain-length and double bond positional specificities of acyl-ACP desaturases. National Plant Lipid Cooperative Symposium. South Lake Tahoe, CA, June 1–4, 1995.

    Google Scholar 

  • Cahoon, E.B., Cranmer, A.M., Shanklin, J., and Ohlrogge, J.B. (1994) Δ6 Hexadecenoic acid is synthesized by the activity of a soluble Δ6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata endosperm. J. Biol. Chem. 269: 27519–27526.

    PubMed  CAS  Google Scholar 

  • Cahoon, E.B., Shanklin, J., and Ohlrogge, J.B. (1992) Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco. Proc. Natl. Acad. Sci. USA 89: 11184–11188.

    Article  PubMed  CAS  Google Scholar 

  • Cao, Y.-Z., and Huang, A.H.C. (1987) Acyl coenzyme A preference of diacylglycerol acyl-transferase from maturing seeds of Cuphea, maize, rapeseed and canola. Plant Physiol. 84: 762–769.

    Article  PubMed  CAS  Google Scholar 

  • DeLuca, V., and Dennis, D.T. (1978) Isoenzyme of pyruvate kinase in proplastids from developing castor bean endosperm. Plant Physiol. 61: 1037–1039.

    Article  CAS  Google Scholar 

  • Dennis, D.T., and Miernyk, J.A. (1982) Compartmentation of nonphotosynthetic carbohydrate metabolism. Ann. Rev. Plant Physiol. 33: 27–50.

    Article  CAS  Google Scholar 

  • Dewey, R.E., Wilson, R.F., Novitzky, W.P., and Goode, J.H. (1994) The AAPT1 gene of soybean complements a cholinephosphotransferase-deficient mutant of yeast. Plant Cell 6: 1495–1507.

    PubMed  CAS  Google Scholar 

  • Downey, R.K., and Craig, R.M. (1964) Genetic controls of fatty acid biosynthesis in rape seed (Brassica napus L.). J. Am. Oil Chem. Soc. 41: 475–478.

    Article  CAS  Google Scholar 

  • Dybing, C.D., and Craig, B.M. (1970) Fatty acid biosynthesis and incorporation into lipid classes in seed and seed tissues of flax. Lipids 5: 522–429.

    Article  Google Scholar 

  • Egli, M.A., Gengenbach, B.G., Gronwald, J.W., Somers, D.A., and Wyse, D.L. (1993) Characterization of maize acetyl-coenzyme A carboxylase. Plant Physiol. 101: 499–506.

    PubMed  CAS  Google Scholar 

  • Fatemi, S., and Hammond, E.F. (1977) Glyceride structure variation in soybean varieties: II Silver ion chromatographic analysis. Lipids 12: 1037–1041.

    Article  CAS  Google Scholar 

  • Fox, B.G., Shanklin, J., Ai, J., Loehr, T.M., and Sanders -Loehr, J. (1994) Resonance raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins. Biochemistry 33: 12776–12786.

    Article  PubMed  CAS  Google Scholar 

  • Fox, B.G., Shanklin, J., Somerville, C., and Munck, E. (1993) Stearoyl-acyl carrier protein Δ9 desaturase from Ricinus communis is a diioron-oxo protein. Proc. Natl. Acad. Sci. USA 90: 2486–2490.

    Article  PubMed  CAS  Google Scholar 

  • Frentzen, M. (1993) Acyltransferases and triacylglycerols. In: Moore, T.S. (ed) Lipid Metabolism in Plants, pp. 195–230, CRC Press, Boca Raton, FL.

    Google Scholar 

  • Griffiths, G., and Harwood, J.L. (1991) The regulation of triacylglycerol biosynthesis in cocoa (Theobroma cacao) L. Planta 184: 279–284.

    Article  CAS  Google Scholar 

  • Griffiths, G., Stobart, A.K., and Stymne, S. (1985) The acylation of sn-glycerol-3-phosphate and the metabolism of phosphatidate in microsomal preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) Seed. Biochem. J. 230: 379–388.

    PubMed  CAS  Google Scholar 

  • Gunstone, F.D., Harwood, J.L., and Padley, F.B. (1994) The Lipid Handbook. Chapman Hall, London.

    Google Scholar 

  • Gurr, M.I., Blades, J., Appleby, R.S., Smith, C.G., Robinson, M.P., and Nichols, B.W. (1974) Studies on seed-oil triglycerides: triglycerides biosynthesis and storage in whole seeds and oil bodies of Crambe abyssinica. Eur. J. Biochem. 43: 281–290.

    Article  PubMed  CAS  Google Scholar 

  • Herman, E.M. (1994) Cell and molecular biology of seed oil bodies. In: Kigel, J., and Gallili, G. (eds) Seed Development and Germination, pp. 195–214, M. Dekker, New York.

    Google Scholar 

  • Hilditch, T.P., and Williams, P.N. (1964) The Chemical Constitutents of Natural Fats. Chapman and Hall, London.

    Google Scholar 

  • Hills, M.J., Dann, R., Lydiate, D., and Sharpe, A. (1994) Molecular cloning of a cDNA from Brassica napus L. for a homologue of acyl-Co-A-binding protein. Plant Mol. Biol. 25: 917–920.

    Article  PubMed  CAS  Google Scholar 

  • Huang, A.H.C. (1992) Oil bodies and oleosins in seeds. Ann. Rev. Plant Physiol. Plant Mol. Biol. 43: 177–200.

    Article  CAS  Google Scholar 

  • Ichihara, K. (1984) sn-Glycerol-3-phosphate acyltransferase in a particulate fraction from maturing safflower seeds. Arch. Biochem. Biophys. 232: 685–698.

    Article  PubMed  CAS  Google Scholar 

  • Ichihara, K. (1991) The action of phosphatidate phosphatase on the fatty acid composition of safflower triacylglycerol and spinach glycerolipids. Planta 183: 353–358.

    Article  CAS  Google Scholar 

  • Ichihara, K., Norikura, S., and Fujii, S. (1989) Microsomal phosphatidate phosphatase in maturing safflower seeds. Plant Physiol. 90: 413–419.

    Article  PubMed  CAS  Google Scholar 

  • Ireland, R.J., and Dennis, D.T (1980) Isozymes of the glycolytic and pentose-phosphate pathways in storage tissues of different oilseeds. Planta 149: 476–479.

    Article  CAS  Google Scholar 

  • James, D.W., and Dooner, H.K. (1990) Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition. Theor. Appl. Genet. 80: 241–245.

    Article  CAS  Google Scholar 

  • James, Jr., D.W., Lim, E., Keller, J., Plooy, I., Ralston, E., and Dooner, H.K. (1994) Directed tagging of the Arabidopsis fatty acid elongation 1 (FAE1) gene with the maize transposon Activator. The Plant Cell 7: 309–319.

    Article  Google Scholar 

  • Jones, A., Davies, H.M., and Voelker, T.A. (1995) Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7: 359–371.

    PubMed  CAS  Google Scholar 

  • Kang, F., and Rawsthorne, S. (1994) Starch and fatty acid synthesis in plastids from developing embryos of oilseed rape (Brassica napus L.). Plant J. 6: 795–805.

    Article  CAS  Google Scholar 

  • Kater, M.M., Koningstein, G.M., Nijkamp, H.J.J., and Stuitje, A.R. (1991) cDNA cloning and expression of Brassica napus enoyl-acyl carrier protein reductase in Escherichia coli. Plant Mol. Biol. 17: 895.

    Article  PubMed  CAS  Google Scholar 

  • Kim, W.K.H., Lopez-Casillas, F., Bai, D.H., Luo, X., and Pape, M.E. (1989) Role of reversible phosphorylation of acetyl-CoA carboxylase in long-chain fatty acid synthesis. Fed. Pro. Fed. Am. Soc. Exp. Biol. 3: 2250–2254.

    CAS  Google Scholar 

  • Knutzon, D.S., Bleibaum, J.L., Nelsen, J., Kridl, J.C., and Thompson, G.A. (1992a) Isolation and characterization of two safflower oleoyl-acyl-carrier protein thioesterase cDNA clones. Plant Physiol. 100: 1751–1758.

    Article  PubMed  CAS  Google Scholar 

  • Knutzon, D.S., Lardizabal, K.D., Nelsen, J.S., Bleibaum, J.L., Davies, G.A., and Metz, J. (1995) Cloning of a coconut endosperm cDNA encoding a 1-acyl sn-gylcerol-3-phosphate acyltransferase which accepts medium chain fatty acids. Plant Physiol, (in press).

    Google Scholar 

  • Knutzon, D.S., Thomson, G.A., Radke, S.E., and Johnson, W.B. (1992b) Modification of Brassica seed oil by antisense expression of a steroyl-acyl carrier protein desaturase gene. Proc. Natl. Acad. Sci. USA 89: 2624–2628.

    Article  PubMed  CAS  Google Scholar 

  • Konishi, T., and Sasaki, Y. (1994) Compartmentalization of two forms of acetyl-CoA carboxylase in plants and the origin of their tolerance toward herbicides. Proc. Natl. Acad. Sci. USA 91: 3598–3601.

    Article  PubMed  CAS  Google Scholar 

  • Kramer, J.K.G., Sauer, F.D., and Pigden, W.J. (1983) High and Low Erucic Acid Rapeseed Oils. Academic Press, Toronto.

    Google Scholar 

  • Kuhn, D.N., Knauf, M., and Stumpf, P.K. (1981) Subcellular localization of acetyl-CoA synthetase in leaf protoplasts of Spinacia oleracea. Arch. Biochem. Biophys. 209: 441–450.

    Article  PubMed  CAS  Google Scholar 

  • Laurent, P., and Huang, A.H.C. (1992) Organ- and development-specific acyl coenzyme A: Lysophosphatidate acyltransferase in palm and meadowfoam. Plant Physiol. 99: 1711–1715.

    Article  PubMed  CAS  Google Scholar 

  • Li, M., Smith, L.J., Clark, D.C., Wilson, R., and Murphy, D.J. (1993) Secondary structure of a new class of lipid body proteins from oilseeds. J. Biol. Chem. 267: 8245–8253.

    Google Scholar 

  • Li, S.-J., and Cronan, Jr., J.E. (1992) The genes encoding the two carboxyltransferase subunits of Escherichia coli acetyl-CoA carboxylase. J. Biol. Chem. 267: 16841–16847.

    PubMed  CAS  Google Scholar 

  • Li, X., Franceschi, V. R. and Okita, T. W. (1993) Segregation of storage protein mRNAs on the rough endoplasmic reticulum membranes of rice endosperm cells. Cell 72: 869–879.

    Article  PubMed  CAS  Google Scholar 

  • Mancha, M., Stobart, K., Lenman, M., Stahl, U., and Stymne, S. (1995) Triacylglycerol turnover in microsomal membranes from developing oil seeds. National Plant Lipid Cooperative Symposium. South Lake Tahoe, CA.

    Google Scholar 

  • Miquel, M., and Browse, J. (1992) Arabidopsis mutants deficient in polyunsaturated fatty acid synthesis. Biochemical and Genetic Characterization of a Plant Oleoyl-Phosphatidylcholine Desaturase. J. Biol. Chem. 267: 1502–1509.

    PubMed  CAS  Google Scholar 

  • Miquel, M., and Browse, J. (1994) Lipid biosynthesis in developing seeds. In: Galili, G., Kigel, J., and Negli, M. (eds) Seed Development and Germination, pp. 169–193, Marcel Dekker, New York.

    Google Scholar 

  • Murphy, D.J. (1994) Designer Oil Crops. VCH, Weinheim.

    Google Scholar 

  • Murphy, D.J., and Stumpf, P.K. (1981) The origin of chloroplastic acetyl coenzyme A. Arch. Biochem. Biophys. 212: 730–739.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, D.R., and Rinne, R.W. (1977) The role of citrate in lipid synthesis in developing soybean cotyledons. Plant and Cell Physiol. 18: 1021–1027.

    CAS  Google Scholar 

  • Nordlund, P., Sjöberg, B.-M., and Eklund, H. (1990) Three-dimensional structure of the free radical protein of ribonucleotide reductase. Nature 345: 593–598.

    Article  PubMed  CAS  Google Scholar 

  • Ohlrogge, J.B. (1994) Design of new plant products: Engineering of fatty acid metabolism. Plant Physiol. 104: 821–826.

    Google Scholar 

  • Ohlrogge, J.B., and Browse, J.A. (1995) Lipid biosynthesis. The Plant Cell 7: 957–970.

    PubMed  CAS  Google Scholar 

  • Ohlrogge, J.B., Browse, J., and Somerville, C.R. (1991) The genetics of plant lipids. Biochim. Biophys. Acta 1082: 1–26.

    Article  PubMed  CAS  Google Scholar 

  • Ohlrogge, J.B., Pollard, M.R., and Stumpf, P.K. (1978) Studies on biosynthesis of waxes by developing jojoba seed tissue. Lipids 13: 203–210.

    Article  CAS  Google Scholar 

  • Okuley, J., Lightner, J., Feldmann, K., Yadav, N., Lark, E., and Browse, J. (1994) The Arabidopsis fad2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 6: 147–158.

    PubMed  CAS  Google Scholar 

  • Palade, G. (1975) Intracellular aspects of the process of protein synthesis. Science 189: 347–358.

    Article  PubMed  CAS  Google Scholar 

  • Pollard, M.R., and Stumpf, P.K. (1980a) Biosynthesis of C20 and C22 fatty acids by developing seeds of Limnanthes alba. Plant Physiol. 66: 649–655.

    Article  PubMed  CAS  Google Scholar 

  • Pollard, M.R., and Stumpf, P.K. (1980b) Long chain (C20 and C22) fatty acid biosynthesis in developing seeds of Tropaeolum majus. Plant Physiol. 66: 641–648.

    Article  PubMed  CAS  Google Scholar 

  • Post-Beittenmiller, D., Jaworski, J.G., and Ohlrogge, J.B. (1991) In vivo pools of free and acylated acyl carrier protein in spinach: Evidence for sites of regulation of fatty acid biosynthesis. J. Biol. Chem. 266: 1858–1865.

    PubMed  CAS  Google Scholar 

  • Post-Beittenmiller, D., Roughan, P.G., and Ohlrogge, J.B. (1992) Regulation of plant fatty acid biosynthesis. Analysis of acyl-coenzyme A and acyl-acyl carrier protein substrate pools in spinach and pea chloroplasts. Plant Physiol. 100: 923–930.

    Article  PubMed  CAS  Google Scholar 

  • Pryde, E.H., and Doty, Jr., H.O. (1981) World fats and oils situation. In: Pryde, E.H., Princen, L.H., and Mukherjee, K.D. (eds) New Sources of Fats and Oils, pp. 5–12, AOCS, Champaign, IL.

    Google Scholar 

  • Reddy, A.S., Nuccio, M.L., Gross, L.M., and Thomas, T.L. (1993) Isolation of a delta-6-desaturase gene from the cyanobacterium Synechocystis sp. Strain PCC-6803 by gain-of-function expression in Anabaena sp. strain PCC-7120. Plant Mol. Biol. 27: 293–300.

    Article  Google Scholar 

  • Reid, E.E., Lyttle, C.R., Canvin, D.T., and Dennis, D.T. (1975) Pyruvate dehydrogenase complex activity in proplastids and mitochondria of developing castor bean endosperm. Biochem. Biophys. Res. Comm. 62: 42.

    Article  PubMed  CAS  Google Scholar 

  • Reid, E.E., Thompson, P., Lyttle, C.R., and Dennis, D.T. (1977) Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids. Plant Physiol. 59: 841–847.

    Article  Google Scholar 

  • Sasaki, Y., Hakamada, K., Suama, Y., Nagano, Y., Furusawa, I., and Matsuno, R. (1993) Chloroplast-encoded protein as a subunit of acetyl-CoA carboxylase in pea plant. J. Biol. Chem. 268:25118–25123.

    PubMed  CAS  Google Scholar 

  • Sasaki, Y., Konishi, T., and Nagano, Y. (1995) The compartmentation of acetyl-coenzyme A carboxylase in plants. Plant Physiol. 108: 445–449.

    PubMed  CAS  Google Scholar 

  • Schmidt, H., Dresselhaus, T., Buck, F., and Heinz, E. (1994) Purification and PCR-based cDNA cloning of a plastidial n-6 desaturase. Plant Mol. Biol. 26: 631–642.

    Article  PubMed  CAS  Google Scholar 

  • Shanklin, J., and Somerville, C. (1991) Stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs. Proc. Natl. Acad. Sci. USA 88: 2510–2514.

    Article  PubMed  CAS  Google Scholar 

  • Shanklin, J., Lindqvist, L., Huang, W., Fox, B., and Schneider, G. (1995) Structure-function studies on desaturase and related hydrocarbon hydroxylases. National Plant Lipid Cooperative Symposium. South Lake Tahoe, CA, June 1–4, 1995.

    Google Scholar 

  • Shanklin, J., Whittle, E., and Fox, B.G. (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenäse. Biochemistry 33: 12787–12794.

    Article  PubMed  CAS  Google Scholar 

  • Shimakata, T., and Stumpf, P.K. (1982a) Isolation and function of spinach leaf b-ketoacyl-[acyl-carrier-protein] synthases. Proc. Natl. Acad. Sci. USA 79: 5808.

    Article  PubMed  CAS  Google Scholar 

  • Shimakata, T., and Stumpf, P.K. (1982b) Purification and characteristics of b-ketoacyl-[acyl-carrier-protein] reductase, b-hydroxyacyl-[acyl-carrier-protein] dehydrase, and enoyl-[acyl-carrier-protein] reductase from Spinacia oleracea leaves. Arch. Biochem. Biophys. 218:77.

    Article  PubMed  CAS  Google Scholar 

  • Shimakata, T., and Stumpf, P.K. (1982c) The procaryotic nature of the fatty acid synthetase of developing Carthamus tinctorius L. seeds. Arch. Biochem. Biophys. 217: 144.

    Article  PubMed  CAS  Google Scholar 

  • Shintani, D.K., and Ohlrogge, J.B. (1995) Feedback inhibition of fatty acid synthesis in tobacco suspension cells. Plant J. 7: 577–587.

    Article  CAS  Google Scholar 

  • Shore, G.C., and Tata, J.R. (1977) Two fractions of rough endoplasmic reticulum from rat liver. J. Cell Biol. 72: 726–743.

    Article  PubMed  CAS  Google Scholar 

  • Shorrosh, B.S., Dixon, R.A., and Ohlrogge, J.B. (1994) Molecular cloning, characterization, and elicitation of acetyl-CoA carboxylase from alfalfa. Proc. Natl. Acad. Sci. USA 91: 4323–4327.

    Article  PubMed  CAS  Google Scholar 

  • Shorrosh, B.S., Roesleer, K.R., Shintani, D., van de Loo, F., and Ohlrogge, J.B. (1995) Structural analysis, plastid localization, and expression of the biotin carboxylase subunit of acetyl-CoA carboxylase from tobacco. Plant Physiol. 108: 805–812.

    Article  PubMed  CAS  Google Scholar 

  • Siggaard-Andersen, M., Kauppinen, S., and von Wettstein-Knowles, P. (1991) Primary structure of a cerulenin-binding beta-ketoacyl-(acyl carrier protein) synthase from barley chloroplasts. Proc. Natl. Acad. Sci. USA 88: 4114.

    Article  PubMed  CAS  Google Scholar 

  • Simcox, P.D., and Dennis, D.T. (1978a) 6-Phosphogluconate dehydrogenase isoenzymes from the developing endosperm of Ricinus communis L. Plant Physiol. 62: 287–290.

    Article  PubMed  CAS  Google Scholar 

  • Simcox, P.D., and Dennis, D.T. (1978b) Isoenzymes of the glycolytic and pentose phosphate pathways in proplastids from the developing endosperm of Ricinis communis L. Plant Physiol. 61:871–877.

    Article  PubMed  CAS  Google Scholar 

  • Simcox, P.D., Reid, E.E., Canvin, D.T., and Dennis, D.T. (1977) Enzymes of the Glycolytic and Pentose Phosphate Pathways in Proplastids from the Developing Endosperm of Ricinus communis L. Plant Physiol. 59: 1128–1132.

    Article  PubMed  CAS  Google Scholar 

  • Slabas, T., Simon, B., Stuitje, T., Rafferty, J., and Rice, D. (1995) Analysis of structure and catalytic mechanism of rape seed enoyl-ACP reductase. National Plant Lipid Cooperative Symposium. South Lake Tahoe, CA, June 1–4, 1995.

    Google Scholar 

  • Slack, C.R., and Browse, J.A. (1984) Synthesis of storage lipids in developing seeds. In: Murray, D.R. (ed) Seed Physiology, pp. 209–244, Academic Press, Orlando, FL.

    Google Scholar 

  • Slack, C.R., Bertaud, W.S., Shaw, B.D., Holland, R., Browse, J.A., and Wright, H. (1980) Some studies on the composition and surface properties of oil bodies from oil seed cotyledons. Biochem. J. 190:551–561.

    PubMed  CAS  Google Scholar 

  • Slack, C.R., Roughan, P.G., and Balasingham, N. (1978) Labeling of glycerolipids in the cotyledons of developing oilseeds by [1-14C]acetate and [2–3H] glycerol. Biochem. J. 179: 421–433.

    Google Scholar 

  • Slack, C.R., Roughan, P.G., and Browse, J.A. (1979) Evidence for an oleoylphosphatidyl-choline desaturase in microsomal preparations from cotyledons of safflower (Carthamus tinctorius) seed. Biochem. J. 179: 649–656.

    Google Scholar 

  • Slack, C.R., Roughan, P.G., Browse, J.A., and Gardiner, S.E. (1985) Some properties of cholinephosphotransferase from developing safflower cotyledons. Biochim. Biophys. Acta 833: 438–448.

    Article  CAS  Google Scholar 

  • Small, D.M. (1986) The Physical Chemistry of Lipids. Plenum Press, New York.

    Google Scholar 

  • Stahl, U., Banas, A., and Stymne, S. (1995) Plant microsomal phospholipid acyl hydrolases have selectivities for uncommon fatty acids. Plant Physiol. 107: 953–962.

    PubMed  Google Scholar 

  • Stobart, A.K., and Stymne, S. (1985) The regulation of the fatty acid composition of the triacylglycerols in microsomal preparation from avocado mesocarp and the developing cotyledons of safflower. Planta 163: 119–125.

    Article  CAS  Google Scholar 

  • Stobart, A.K., Stymne, S., and Hoglund, S. (1986) Safflower microsomes catalyse oil accumulation in vitro: A model system. Planta 169: 33–37.

    Article  CAS  Google Scholar 

  • Strittmatter, P., Spatz, L., Corcoran, D., Rogers, M.J., Setlow, B., and Redline, R. (1974) Purification and properties of rat liver microsomal stearyl coenzyme A Desaturase. Proc. Natl. Acad. Sci. USA 71: 4565–1569.

    Google Scholar 

  • Stymne, S., and Appleqvist, L.A. (1980) The biosynthesis of linoleate and a-linolenate in homogenates from developing soy bean cotyledons. Plant Sci. Lett. 17: 287–294.

    Article  CAS  Google Scholar 

  • Stymne, S., and Stobart, A.K. (1984) Evidence for the reversibility of the acyl-CoA: Lysophos-phatidylcholine acyltransferase in the microsomes of developing safflower cotyledons and rat liver. Biochem. J. 223: 305–314.

    PubMed  CAS  Google Scholar 

  • Stymne, S., and Stobart, A.K. (1986) Biosynthesis of g-linolenic acid in cotyledons and microsomal preparations of the developing seeds of common borage (Borago officinalis). Biochem. J. 240: 385–393.

    PubMed  CAS  Google Scholar 

  • Stymne, S., and Stobart, A.K. (1987) Triacylglycerol biosynthesis. In: Stumpf, P.K., and Conn, E.E. (eds) The Biochemistry of Plants, Vol. 9, pp. 175–214, Academic Press, New York.

    Google Scholar 

  • Tai, H., and Jaworski, J.G. (1993) 3-Ketoacyl carrier protein synthase III from spinach (Spinacea oleracea) is not similar to other condensing enzymes of fatty acid synthease. Plant Physiol. 103: 1361–1367.

    Article  PubMed  CAS  Google Scholar 

  • Thomas, T.L., Nunberg, A., Reddy, A.S., Nuccio, M.L., and Beremand, R (1995) Cloning and expression of A6-desaturase genes in transgenic plants. National Plant Lipid Cooperative Symposium. South Lake Tahoe, CA, June 1–4, 1995.

    Google Scholar 

  • Tzen, J.T.C., and Huang, H.C. (1992) Surface structure and properties of plant seed oil bodies. J. Cell Biol. 117:327–335.

    Article  PubMed  CAS  Google Scholar 

  • van de Loo, F.J., Broun, P., Turner, S., and Somerville, C. (1995) An oleate 12-hydroxylase from Ricinus communis is a fatty acyl desaturase homolog. Proc. Natl. Acad. Sci. USA (in press).

    Google Scholar 

  • van de Loo, F.J., Fox, B.C., and Somerville, C.R. (1993) Unusual fatty acids. In: Moore, TS. (ed) Lipid Metabolism in Plants, pp. 91–126, CRC Press, Boca Raton, FL.

    Google Scholar 

  • Voelker, T.A., Worrell, A.C., Anderson, L., Bleibaum, J., Fan, C., Hawkins, D.J., Radke, S.E., and Davies, H.M. (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257: 72–74.

    Article  PubMed  CAS  Google Scholar 

  • Vogel, G., and Browse, J. (1995) Cholinephosphotransferase and diacylglycerol acyltrans-ferase: Substrate specificities at a key branch point in seed lipid metabolism. Plant Physiol. 110: (in press).

    Google Scholar 

  • Wakil, S.J., Stoops, J.K., and Joshi, V.C. (1983) Fatty acid synthesis and its regulation. Ann. Rev. Biochem. 52: 537–579.

    Article  PubMed  CAS  Google Scholar 

  • Wanner, G., Formanek, H., and Theimer, R.R. (1981) The ontology of lipid bodies (sphero-somes) in plant cells. Planta 151: 109–123.

    Article  Google Scholar 

  • Weiss, T.J. (1983) Food Oils and Their Uses. AVI Publishing, Westport, CT.

    Google Scholar 

  • Wiberg, E., Banas, A., and Stymne, S. (1995) Partitioning of medium chain fatty acids between membrane and storage lipids in laurate producing rape. National Plant Lipid Cooperative Symposium. South Lake Tahoe, CA, June 1–4, 1995.

    Google Scholar 

  • Wiberg, E., Tillberg, E., and Stymne, S. (1994) Substrates of diacylglycerol acyltransferase in microsomes from developing oil seeds. Phytochemistry 36: 573–577.

    Article  CAS  Google Scholar 

  • Yadav, N.S., Wierzbicki, A., Aegerter, M., Caster, C.S., Pérez-Grau, L., Kinney, A.J., Hitz, W.D., Booth, Jr., R., Schweiger, B., Stecca, K.L., Allen, S.M., Blackwell, M., Reiter, R.S., Carlson, T.J., Russell, S.H., Feldmann, K.A., Pierce, J., and Browse, J. (1993) Cloning of higher plant omega-3 fatty acid desaturases. Plant Physiol. 103: 467–476.

    Article  PubMed  CAS  Google Scholar 

  • Yamada, M., and Usami, Q. (1975) Long chain fatty acid synthesis in developing castor bean seeds IV. The synthetic system in proplastids. Plant and Cell Physiol. 16: 879–884.

    CAS  Google Scholar 

  • Yamada, M., Usami, Q., and Nakajima, K. (1974) Long chain fatty acid synthesis in developing castor bean seeds II. Operation of the path from sucrose to long chain fatty acids in a subcellular particulate system. Plant and Cell Physiol. 15: 49–58.

    CAS  Google Scholar 

  • Yatsu, L.Y., and Jacks, T.J. (1972) Spherosome membranes. Plant Physiol. 49: 937–947.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Biological Chemistry, Washington State University, Pullman, Washington, 99164-6340, USA

    John Browse

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  1. John Browse
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  1. Department of Plant Sciences, University of Arizona, Tucson, Arizona, USA

    Brian A. Larkins

  2. Laboratory of Plant Cell and Molecular Biology, University of Florida, Gainesville, Florida, USA

    Indra K. Vasil

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Browse, J. (1997). Synthesis and Storage of Fatty Acids. In: Larkins, B.A., Vasil, I.K. (eds) Cellular and Molecular Biology of Plant Seed Development. Advances in Cellular and Molecular Biology of Plants, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-8909-3_11

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  • DOI: https://doi.org/10.1007/978-94-015-8909-3_11

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