Abstract
Culture media from a number of rhizobial strains, including the type-strains of 8 major cross-inoculation groups, were analyzed for cytokinin content. Cytokinins were partially purified by chromatography on Amberlite XAD-2, then on Sephadex LH-20. The tobacco callus assay, HPLC and/or immunoassay were used for cytokinin analysis. All strains of rhizobia examined produced at least 2 cytokinin-active compounds, with total cytokinin activity ranging from 1 to several µg kinetin equivalents per liter of culture filtrate. There were both qualitative and quantitative differences between rhizobial species. The cytokinin profiles of most strains included zeatin or its derivatives. Addition of adenine, seed extract or flavonoid inducers to the culture medium altered cytokinin synthesis. Preliminary genetic data do not support nod gene involvement in cytokinin synthesis under noninducing conditions. Growth pouch experiments showed increased nodulation of soybean plants treated with trans-zeatin.
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© 1991 Springer Science+Business Media Dordrecht
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Taller, B.J., Sturtevant, D.B. (1991). Cytokinin Production by Rhizobia. In: Hennecke, H., Verma, D.P.S. (eds) Advances in Molecular Genetics of Plant-Microbe Interactions Vol. 1. Current Plant Science and Biotechnology in Agriculture, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7934-6_33
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DOI: https://doi.org/10.1007/978-94-015-7934-6_33
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