Abstract
To incite a systemic soft rot disease on saintpaulia plants, E. chrysanthemi requires a set of pectinolytic activities ( PL ) and a high affinity iron transport system to be functional. Chrysobactin, the siderophore of this system [ 10 ], is believed to provide iron to invading bacteria in plant vessels, an environment which is low in free iron. This uptake system is negatively controlled by the transcriptional regulator Cbr, effective under non restrictive iron conditions. Here, we show that iron stress also stimulates the production of all pectate lyase activities. In wild type cells, this fact is correlated with increased levels of PL specific mRNA. In the Cbr- background, high levels of PL mRNA were also detected in replete iron conditions, but enzymatic activity was no longer found to be proportional.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andro T., Chambost J P., Kotoujansky A., Cattaneo J., Bertheau Y., Barras F., Van Gijsegem F. and Coleno A. ( 1984 ) Mutants of E. chrysanthemi defective in secretion of pectinases and cellulases, . J. Bacteriol. 160, 1199–1203.
Apel K. and Kloppstech K. (1978) Eur. J. Biochem. 85, 581–588.
Bertheau Y., Madjidi-Hervan E., Kotoujansky A., Nguyen-The C, Andro T. and Coleno A. (1984) Detection of depolymerase isoenzymes after electrophoresis or electrofocusing or in titration curves, Anal. Biochem. 139, 383–389.
Boccara M. and Châtain V. (1989) Regulation and role of E. chrysanthemi 3937 pectin methylesterase, J. Bacteriol. 171, 4085–4087.
Boccara M., Diolez A., Rouve M. and Kotoujansky A. (1988) The role of individual pectate lyases of E. chrysanthemi in pathogenicity on saintpaulia plants, Physiol. Mol. Plant Pathol. 33, 95–104
Enard C, Diolez A. and Expert D. (1988) Systemic virulence of E. chrysanthemi 3937 requires a functional iron assimilation system J. Bacteriol. 170, 2419–2426.
Enard C, Franza T., Neema C, Gill P. R., Persmark M., Neilands J. B. and Expert D. (1990) The requirement of chrysobactin dependant iron transport for virulence incited by E. chrysanthemi on Saintpaulia ionantha. J. Plant Nutr. in press.
Hugouvieux-Cotte-Pattat N. and Robert-Baudouy J. (1989) Isolation of E. chrysanthemi mutants altered in pectinolytic enzyme production, Mol. Microbiol. 3, 1587–1597.
Moran F., Nasuno S. and Starr M. P. (1968) Extracellular and intracellular polygalacturonic acid trans-eliminases of E. carotovora. Arch. Biochem. Biophys. 123, 298–306.
Persmark M., Expert D.and Neilands J. B.(1989) Isolation, characterization and synthesis of chrysobactin, a compound with siderophore activity from E. chrysanthemi. J. Biol. Chem. 264, 3187–3196.
Reverchon S., Van Gijsegem F., Rouve M., Kotoujansky A. and Robert-Baudouy J. (1986) Organization of a pectate lyase gene family in E. chrysanthemi, Gene 29, 215–224.
Weinberg E. D. (1984) Iron withholding: a defense against infection disease, Physiol. Rev. 64, 65–102.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Sauvage, K., Franza, T., Expert, D. (1991). Iron as a Modulator of Pathogenicity of Erwinia Chrysanthemi 3937 on Saintpaulia Ionantha . In: Hennecke, H., Verma, D.P.S. (eds) Advances in Molecular Genetics of Plant-Microbe Interactions Vol. 1. Current Plant Science and Biotechnology in Agriculture, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7934-6_15
Download citation
DOI: https://doi.org/10.1007/978-94-015-7934-6_15
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-4083-1
Online ISBN: 978-94-015-7934-6
eBook Packages: Springer Book Archive