Abstract
In vitro tissue culture of inbred species such as tomato has led to regeneration, and phenotypic variation has been observed amongst regenerants.
Genetic control was studied after verification of chromosome number. Self-fertilisation revealed 5G % of plants to be mutant for 1 or more of 5 characters noted. Another group (17%) presented new characteristics but did not give segregating progeny, thus behaving as if homozygous.
Some of the progeny from reciprocal crosses amongst this last group and with the control were asymmetric (particularly paternally oriented) and showed heterosis-like effects, which we term “transgression” because nuclei are postulated to be isogenic.
The stability of transgressive effects after selfing the crosses between variants, as well as their asymmetrical behaviour in reciprocal crosses, imply that this heterosis-like phenomenon is neither directly caused by heterozygous coding DNA, nor explicable in terms of cytoplasmic effects alone. The evidence suggests heritable changes in cytoplasmic or nuclear genetic elements other than coding genes. We term this “epigenic” to include asymmetrical heredity involving cell elements other than the strictly cytoplasmic, and we seek to more precisely define the notion of epigenetics.
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Sibi, M. (1986). — Non Mendelian Heredity — Genetic Analysis of Variant Plants Regenerated from in vitro Culture : Epigenetics and Epigenic. In: Somaclonal Variations and Crop Improvement. Advances in Agricultural Biotechnology, vol 20. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7733-5_7
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DOI: https://doi.org/10.1007/978-94-015-7733-5_7
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