Abstract
Immature embryo-derived tissue cultures of four Triticum aestivum L. varieties have been sutied for their ability to adapt to different osmotic stresses. The increased carbohydrate content in the medium (sucrose, glucose, mannitol added) decreased growth, water content and plant regeneration and increased the phosphorus content. Free amino acid and polyamine contents of “Chinese Spring”, “Arthur” and “Capelle Desprez” were determined in the presence of 8%, 12% and 14% mannitol. The levels of free amino acids and polyamines showed characteristical different changes in cultivar “Capelle Desprez” comparing to those of “Chinese Spring” and “Arthur”. In the case of “Capelle Deprez”, only those amino acids showed some changes which are involved in the polyamine synthesis (LYS, ARG) except proline, that gave an identical monotonly increased curve in all three cultivars investigated. Most of the amino acids determined in “Chinese Spring” and “Arthur” showed a sygmoid saturation-type curve. The changes in the concentration of putrescine and cadaverine were parallel in all samples. In “Chinese Spring” and “Arthur”, saturation-types curves were observed, while in “Capelle Desprez”, monotone increase of polyamines was found.
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© 1986 Springer Science+Business Media Dordrecht
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Gåbor, G., Simon-Sarkadi, L., Békés, F., Erdei, L. (1986). Genotype Specific Changes in Amino Acid and Polyamine of Wheat Tissue Culture Induced by Osmotic Stress. In: Somaclonal Variations and Crop Improvement. Advances in Agricultural Biotechnology, vol 20. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7733-5_16
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DOI: https://doi.org/10.1007/978-94-015-7733-5_16
Publisher Name: Springer, Dordrecht
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